Running Protocols
Priming the system
Thermo Fisher Scientific
Thermo Scientific Wellwash Versa User Manual
47
Caution
Ensure that the plate is not covered.
▲
Caution
Do not limit the plate clamp operation.
▲
Insert the 96- or 384-well plate to be washed onto the plate carrier
(Figure 5–33). Position A1 of the plate should correspond to position
A1 marked on the plate carrier. The plate clamp will hold the plate in
position during the run.
Figure 5–33.
Inserting the microplate
Caution
Ensure that the strips in strip plates are positioned in the
microplate as selected in the user interface, otherwise spilling may occur
and the instrument may become contaminated.
▲
Caution
For proper performance, all wells in a strip should have an
equal amount of liquid. It is recommended to use deionized distilled
water in unused wells on the strip.
▲
Press the
PRIME
button to fill the liquid tubing completely from the
selectable buffer bottle intake tube to the wash head tips. If you prime
for the first time, fill the buffer bottle completely to ensure proper
priming. The default priming volume is
30 ml
to fill the liquid tubing
properly. On subsequent priming occasions when the tubing contains
the desired liquid, you can use a smaller priming volume. The dead
volume of the liquid tubing is 20 ml (see Figure 5-34).
If the instrument will be left to stand for a longer time, priming must be
performed with air to remove all liquid from the system. Raise the tube
from the liquid to allow air to enter the tube.
The default volume used during priming is defined in the Settings >
Prime & rinse parameters >
Prime volume (Prime button) (ml)
menu.
Refer to “Prime & rinse parameters” on page 81.
Priming the
system
A1
Plate clamp
