Appendix A
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General Instrument Information
Thermo Scientific
Orion AquaMate Spectrophotometer User Manual
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205
Parameter
Description
Number of Samples Enters the number of samples to be measured in the test (Not available in
Kinetics or Scanning)
Number of Standards Enters the number of standards to be measured for the standard curve
Printer
Selects the output mode as RS-232 or Parallel (Utility)
Protein Factor
Enters the factor to calculate protein concentration (DNA Bio tests)
Ref. Wavelength
Enters a reference wavelength value; for each reported measurement, measures
the analytical wavelength and reference wavelength
Reported measurement = Abs @ Analytical WL – Abs @ Reference WL
Ref. Wavelength
Correction
Turns reference wavelength correction on or off
Run Standard
Goes to the standards entry screen
Run Test
Goes to the data collection screen
Sample Positioner
Selects the type of Sample Positioner:
1 Cell = no movement; zeros and measures sample in same position
Manual 6 = vial holder carousel moved by sample positioner keypad; always
zeros on position B, then returns to set position to start measurement
Auto 3 = vial holder carousel automatically moved to B, 2, 4 (always zeros on
position B, then goes to position 2 to start measurement)
Auto 6 = vial holder carousel automatically moved to B,1,2,3,4,5 (always zeros
on position B, then goes to position 1 to start measurement)
Sample Volume
Enters the total volume of sample (under Dilution Multiplier in some Bio Tests)
Save Test
Saves all parameters of the current test in internal memory for later recall
Scan Speed
Selects the speed (nm/min) for a scan as Slow, Medium or Fast (Scanning)
Screen Contrast
Improves visibility of the display by changing the contrast between the
background and text (Utility)
Select Test
Tags the highlighted test name with “>” to include the test in the SmartStart menu
(Utility Stored Tests Directory)
Set Max. X
Set Min. X
Sets the cursor position in the graph as the minimum X and the maximum X
values for recalculating rate (Kinetics)
Set nms
Use to enter and edit the wavelength and factor values
Set Options
Selects the factor entry or the baseline to calculate area under the peak in the
graph (Scanning)
Setup Correction
Initiates the procedure to collect the data necessary to correct for absorbance
differences between vials or cuvettes
Slope
Enters abs/concentration value (Standard Curve)
Smoothing
Turns data smoothing on and off (Scanning)
Software Revision
Displays the version of firmware in the instrument (Utility)
SRE Tolerance
Acceptable minimum stray light
Standard
Concentrations
Enters the concentration of standards used to generate the standard curve for
the test
Standby
Selects the time since the last keystroke or instrument activity; powers down the
unit to save lamp life (Utility)
Start Wavelength
Enters the beginning wavelength for a scan (Scanning)