Chapter 8
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Absorbance, % Transmittance and Concentration Measurements
Thermo Scientific
Orion AquaMate Spectrophotometer User Manual
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155
a.
Press the Add nm function key, use the numeric keypad to enter the wavelength
value and press the enter key.
b.
If the measurement mode is set to Conc/Factor, use the numeric keypad to enter the
factor value and press the enter key.
c.
Repeat for each wavelength and factor.
5.
Press the Run Test function key.
Taking Measurements with Multiwavelength Method
Once the parameters have been set for the multiwavelength test:
1.
Press the Run Test function key.
2.
Prepare the blank and samples that will be measured.
3.
If Auto 6 or Auto 3 is selected for the Sample Positioner setting:
a.
Place the blank and samples in the appropriate positions on the carousel.
b.
Confirm that the blank and samples are in the appropriate positions on the carousel
and press the enter key.
c.
Once the blank and samples have been measured, remove them from the carousel.
d.
To measure additional samples, press the Measure Sample function key and repeat
steps 3a through 3c.
4.
If Manual 6 or Single Cell Holder is selected for the Sample Positioner setting:
a.
Place the blank in the B position on the vial holder carousel and press the Measure
Blank function key. Once the blank has been measured, remove it from the carousel.
b.
Place the sample in the appropriate position on the vial holder carousel. If needed,
use the Sample Positioner keypad to set the vial location. Press the Measure
Sample function key. Repeat for each sample.
5.
When all of the samples have been measured, press the Save Data function key and
follow the onscreen prompts to save the sample measurements to the USB memory stick.