9.2
System
Validation
3.
Procedure
Outline
of procedure is described
as
below:
(1)
Set the
flow
rate of the pump to
and column oven
temperature
to
Start
pump
flow
and temperature control.
Then, confirm that the solvent comes out
the outlet tubing
of the detector and no leak
of
solvent
is
observed at the
connection.
Set the parameters
of the
RID
detector.
AUX RANGE
:
2 (1
x
RESPONSE
:
5
WIDTH
:
5
SLOPE
:
:
100,000
:
7
SPEED
Set the parameters
of
the data processor.
Monitor the baseline. When a stable baseline
is
obtained,
press the zero point adjustment key of the detector. Then,
inject
of
mobile phase and confirm no peak
is
observed.
Inject 10
of
test sample solution. Repeat measurement at
least five times.
Obtain the chromatographic data
of retention time, peak
area,
and peak height
five successive analyses.
Calculate the average
of the data and
using
the
equations
as
shown below.
SD
=
XA
=
+ ...... +
5
=
(SD
XA)
*
:
Data
XA
:
Average
SD
:
Standard deviation
:
Relative standard deviation
9-1
1
RID-1OA
Summary of Contents for RID-10A
Page 19: ...Chapter 2 Parts List c v 3 2 1 Parts and Accessories 2 2 1CONTENTS 1 2 1 RID 1OA...
Page 63: ...c Chapter 6 Initial Performance Test 1 6 1 System Performance Check 6 2 6 1 RID 1OA...
Page 102: ...Chapter I 1 Specifications 11 1 RID 10A Specifications 11 2 I CONTENTS I 11 1 RID 1OA...
Page 105: ...Parts I 12 1 Consumablesand Repair Parts 12 2 12 2 Optional Parts 12 4 12 1 RID 1OA...
Page 109: ...Chapter 13 Reference I i L 13 1 Solvent Characteristics 13 2 I 13 1 RID 1OA...
