Shimadzu RID-10A, User Manual

Page: 89 / 112

9.1 Component Validation
4.
Checking the span
Content of inspection
This test checks whether the refractive index value is accurately
measured and displayed by testing the standard sample whose
index is already
known.
The test requires the following reagents and equipment.
Distilled water
Standard sample (Glycerin solution)
Syringe
Data processor
Refer to the section 8.2 Span Adjustment.
Test procedure
Set the parameters
of
the detector as follows.
CELL TEMP:
polarity:
+
mode: A (When performing mode A span adjustment)
Set the detector
so
that the plot full scale of the data processor
becomes 128 x
AUX RANGE of the 2 (or 4)
of the Chromatopac: 7
(or 9)
Use the syringe and adapter to deliver distilled water to the
(2)
(3)
inlet port. During delivery, press the and R keys
to flow the water to the reference flow lines.
After the inside of the sample cell is replaced
with distilled water,
OFF
by pressing the
and keys again.
When the baseline is stabilized, press the and
keys to perform optical balance adjustment.
Press the key and record the baseline level when the
cell is filled with distilled water.
Inject the standard solution from
the
inlet port. The baseline
moves up ward by 80% of the full scale.
(4)
( 5 )
(6)
(7)
Record the baseline level when the cell is filled with
standard solution. When the Chromatopac is used, press the
PLOT
key to record the baseline.
(8)
Read the difference of the baseline level between the
distilled water and the standard solution, and convert the
value into unit.
9-4