9.2 System Validation
(d)
Sample
*
Anthracene in acetonitrile
[Method for sample preparation]
Place
of anthracene in the volumetric flask of
capacity and add acetonitrile of HPLC grade
to it.
(e)
Isopropyl alcohol
Water (HPLC grade or equivalent)
(2)
(3)
Check the connection
of the units. Refer to the instruction
manuals for details of the connection of each unit.
Before installing the column, observe tubing connection of
the LC system. Use tubing with
I.D.
or less from the
outlet of auto injector to the column inlet and
from the column
outlet
to the detector inlet. Length of the tubing should
be
30cm or less
to minimize the
band broadening.
Flush the flow line with appropriate solvents dependent on
the operation status of the system. General guideline is
shown below.
To
flush the flow line with solvents, connect
the inlet and outlet tubing of the column directly using
a
proper
union. Column should be connected after flushing.
(4)
Newly installed system
Flush the flow line with isopropyl alcohol, then with water at
a flow rate of
2
for ten minutes.
When
low polarity solvent (such as hexane) is used
as
the mobile phase in the system
Flush the flow line with isopropyl alcohol, then with water at
a flow rate of
2
for ten minutes.
When mixture of water and organic solvent, or
organic solvent miscible with water (methanol,
acetonitrile, etc.), or buffer solution is used as the
mobile phase in the system
Flush the flow line with water at a flow rate
of 2
for
ten minutes.
( 5 )
Set the mobile phase (b) and flush the flow line, then connect
the column (c) to the LC system.
9-10
RID-1OA
Summary of Contents for RID-10A
Page 19: ...Chapter 2 Parts List c v 3 2 1 Parts and Accessories 2 2 1CONTENTS 1 2 1 RID 1OA...
Page 63: ...c Chapter 6 Initial Performance Test 1 6 1 System Performance Check 6 2 6 1 RID 1OA...
Page 102: ...Chapter I 1 Specifications 11 1 RID 10A Specifications 11 2 I CONTENTS I 11 1 RID 1OA...
Page 105: ...Parts I 12 1 Consumablesand Repair Parts 12 2 12 2 Optional Parts 12 4 12 1 RID 1OA...
Page 109: ...Chapter 13 Reference I i L 13 1 Solvent Characteristics 13 2 I 13 1 RID 1OA...
