PerkinElmer RamanStation 400 Getting Started Manual Download Page 44

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44 . RamanStation 400 Series Getting Started Guide

Unpacking the Instrument

When the instrument has been delivered, please move it with care and avoid shaking or

dropping the spectrometer. Allow the instrument to equilibrate to room temperature for at

least 12 hours before opening. The instrument may have been stored at low temperature

prior to delivery and you should allow it to equilibrate to stop moisture condensing on the

internal electronics.

When unpacked, check that you have the following parts and check for any physical damage.

If there is any damage, do not continue with the installation. Contact your PerkinElmer

Service Representative immediately.

Part Number

Description

L1320004/L1320005

L1320008/L1320009

L1320032/L1320033

L1320034/L1320035

RamanStation 400/400F with 785 nm laser, open electrode CCD

detector and Spectrum Std software

RamanStation 400/400F with 785 nm laser, open electrode CCD

detector and Spectrum ES software

RamanStation 400/400F with 785 nm laser, back illuminated deep

depletion CCD detector and Spectrum Std software

RamanStation 400/400F with 785 nm laser, back illuminated deep

depletion CCD detector and Spectrum ES software

LX108870

LX108871

Spectrum Std for Raman Software Kit

Contains the Spectrum Std Software CD (LX100884), the Raman

Instrument Installation Disk (LX108925), the Raman Instrument

Configuration Disk (LX108869) and the RamanStation 400 Series

Getting Started Guide (L1321801)
Spectrum ES for Raman Software Kit

Contains the Spectrum ES Software CD (LX100881), the Raman

Instrument Installation Disk (LX108925), the Raman Instrument

Configuration Disk (LX108869) and the RamanStation 400 Series

Getting Started Guide (L1321801)

L1323503

Laser key (x2)

L1320821

USB Cable

L1321802

RamanStation Mains PSU

L1320263

Cover for Fiber Probe Couplers

(L1320005/L1320009/L1320033/L1320035 only)

L1320264

External Interlock Override

(L1320005/L1320009/L1320033/L1320035 only)

L1320295

L1320296

XYZ Stage with standard lens snout

XYZ Stage with Polarization Accessory

L1320227

Versatile Sample Holder

Summary of Contents for RamanStation 400

Page 1: ...RamanStation 400 Series Getting Started Guide MOLECULAR SPECTROSCOPY...

Page 2: ...ut not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in con...

Page 3: ...History Theory and Instrumentation 29 The History of Raman Spectroscopy 30 Raman Theory 31 The RamanStation 400 Series 33 Spectrograph Detector Systems 35 Preparing Your Facility 37 Specifications 38...

Page 4: ...Motorized XYZ Sample Stage 94 Fiber Optic Probe SOPs 96 SOP 7 Selecting the Fiber Optic Probe 96 SOP 8 Sample Positioning when using a Fiber Optic Probe 97 Calibration and Performance Verification SO...

Page 5: ...Introduction...

Page 6: ...n spectroscopy Preparing your Facility This chapter shows you how to position your RamanStation 400 and what utilities are required for example electricity temperature control Installing your RamanSta...

Page 7: ...to press two keys at the same time for example ALT F All eight digit numbers are PerkinElmer part numbers unless stated otherwise The term RamanStation 400 Series means the RamanStation 400 and the R...

Page 8: ...o en otros En recuadros ste se proporciona informaci n sobre este tipo de circunstancias Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provo...

Page 9: ...os recuadros como ste se proporciona informaci n sobre este tipo de circunstancias Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages...

Page 10: ...10 RamanStation 400 Series Getting Started Guide...

Page 11: ...Safety Practices...

Page 12: ...lieved to be correct and complete PerkinElmer shall not be liable for errors contained herein nor damages concerned with the use of this document PerkinElmer does not accept liability for the interpre...

Page 13: ...s Temperature 15 C to 30 C Relative Humidity 75 maximum non condensing WARNING If the equipment will be used at temperatures exceeding 35 o C we recommend that you contact your PerkinElmer Service Rep...

Page 14: ...ltitude up to 2000 m Ambient temperatures of 5 C to 40 C A maximum ambient relative humidity of 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains fluctuations no...

Page 15: ...Safety Ensure that there is at least 15 cm 6 between the rear of the instrument and the nearest wall Ensure that the power supply unit PSU vents or fan do not become obstructed Figure 1 Figure 1 Powe...

Page 16: ...vers or service panels on the instrument appear to be damaged turn the instrument off secure the instrument against any unauthorized operation and contact PerkinElmer for advice before using it again...

Page 17: ...earth socket The ground must be a non current carrying wire that is connected to the earth ground at the main distribution box The power points sockets for the instrument PSUs should be easily access...

Page 18: ...Series complies with the EMC standard EN61326 EMC standard for electrical equipment for measurement control and laboratory use and EN55011 ISM Class A rf emissions FCC rules and regulations This prod...

Page 19: ...85 nm which is classified as a Class 3B laser source During normal operation laser radiation is accessible from the end of the probe The output energy should be enclosed whenever possible using an enc...

Page 20: ...device with a captive key control switch such that the key cannot be removed from the lock except in the OFF position The key should be removed from the laser when it is not in use and kept in a safe...

Page 21: ...nuator cover to avoid unnecessary exposure The laser should also be turned off and the probe cap replaced when the system is not in use AVOID DIRECT EXPOSURE TO THE BEAM Figure 2 Schematic of the fibe...

Page 22: ...ation of lasers are suggested for laser operations When the probe is in use always ensure that no stray laser radiation is directed at people in the vicinity Use protective laser safety goggles with a...

Page 23: ...etails In particular the use of an ultra long working distance lens adaptor part number L1320071 provides a working distance of 25 mm Where different focal lengths are used the NOHD should be calculat...

Page 24: ...only be performed by a PerkinElmer Service Engineer during service maintenance or repair of the instrument observing Class 3B laser safety precautions at all times External Interlock System NOTE This...

Page 25: ...voltage and current values MAXIMUM OUTPUT 250mW PULSE DURATION CONTINUOUS WAVE EMITTED WAVELENGHS 785nm THIS LASER PRODUCT CONFORMS TO IEC 60825 1 1993 21 CFR 1040 10 1040 11 LASER RADIATION AVOID EXP...

Page 26: ...owing labels are displayed on the exterior of the instrument NOTE If you have a deep depletion CCD installed in your RamanStation 400 the ratings label will include modified power voltage and current...

Page 27: ...self and damage to the instrument switch OFF all components in the system and disconnect them from the line power supply before you alter or make any new electrical connections The only fuses that you...

Page 28: ...28 RamanStation 400 Series Getting Started Guide...

Page 29: ...Raman History Theory and Instrumentation...

Page 30: ...observed the scattering from water and alcohol visually In addition to the blue incident light a faint green light also scattered later named Stokes scattering By the 1930 s much of the theory underl...

Page 31: ...Stokes scattering or shorter wavelengths higher frequencies Anti Stokes scattering With Stokes scattering some of the incident photons give up a portion of energy to the molecules and are detected at...

Page 32: ...otational energy of the molecule The frequency shifts from the frequency of the incident beam are displayed on the x axis of a Raman spectrum generally in relative wavenumbers otherwise known as Raman...

Page 33: ...ptic probe Figure 8 The RamanStation 400 Series The instrument Figure 8 is composed of a laser mirrors filters lenses spectrograph and a CCD detector The laser supplies light through an objective lens...

Page 34: ...34 RamanStation 400 Series Getting Started Guide Figure 9 Schematic of the RamanStation 400 Series Figure 10 Schematic of the optical head optical layout...

Page 35: ...ometers The system contains two components the Echelle spectrograph and a two dimensional CCD detector Figure 11 The system works by dispersing the light in two dimensions by using two gratings The fi...

Page 36: ...a full spectral range There are no moving parts in the spectrograph which improves instrument reliability ruggedness and the quality of calibration The absence of moving parts also means that as you a...

Page 37: ...Preparing Your Facility...

Page 38: ...power is erratic please use an uninterruptible power supply UPS Incorrect shutdown power fluctuations or brown outs may damage the spectrometer RamanStation 400 RamanStation 400F Weight 45 kg Height...

Page 39: ...ing able to create new data files CD ROM drive Ethernet network connection A keyboard and USB or PS 2 style mouse Hi Speed USB 2 0 port x2 Serial RS232 port Software Requirements Spectrum requires tha...

Page 40: ...floor coverings to minimize static discharge Floor vibrations or noise from heavy manufacturing equipment can affect the performance of the instrument Intense magnetic fields should be at least 5 5 m...

Page 41: ...5 kg Laboratory benching must be strong enough to support the combined weight of the instrument and samples typically 46 kg without warping or wobbling The bench must be at least 640 mm 25 in depth Th...

Page 42: ...42 RamanStation 400 Series Getting Started Guide...

Page 43: ...Installing Your RamanStation 400...

Page 44: ...or and Spectrum ES software OR RamanStation 400 400F with 785 nm laser back illuminated deep depletion CCD detector and Spectrum Std software OR RamanStation 400 400F with 785 nm laser back illuminate...

Page 45: ...ntroller PSU 09410022 RS232 Cable If you have a deep depletion CCD installed in your RamanStation 400 L1320032 L1320033 L1320034 L1320035 you will also receive L1323525 Deep Depletion PSU L1323010 Rec...

Page 46: ...ifting techniques WARNING To lift and or move the instrument safely requires two people WARNING Do not lift the RamanStation 400 using the instrument console located on the left hand side of the instr...

Page 47: ...XYZ stage provided with the instrument requires an XYZ stepper motor controller 1 Attach the stage controller box L1320819 to the Sample Accessory connector on the rear panel of the instrument 2 Atta...

Page 48: ...to collect data even if data is being collected using the fiber optic probe You must attach the External Interlock Override L1320264 into the 7 way DIN connector labeled EXT INTERLOCK on the rear pan...

Page 49: ...he following conditions are met No fiber optic probe is connected to the instrument The fiber optic probe cover assembly L1320263 is attached to the instrument and completely encloses the EXC and COL...

Page 50: ...er cords from the PC and the monitor into the mains electrical supply and then switch on 2 If you are prompted to login to Windows when you start the PC log in If your PC was provided by PerkinElmer y...

Page 51: ...can be obtained from PerkinElmer A laser safety officer or Lab manager can control use of the instrument with these keys 3 If your instrument is fitted with a deep depletion CCD connect the 7 5 V PSU...

Page 52: ...R socket and then switch on Figure 20 The instrument power cable has a red band around the end The Main Power socket is indicated by a red dot Do not connect to the AUX POWER connector Figure 20 Conne...

Page 53: ...iate between the PC and triggered fiber optic probe are NOT connected The USB cable from your alignment camera if fitted is not connected into the USB socket in the sample compartment of the RamanStat...

Page 54: ...ation program does not start automatically from the Start Menu select Run The Run dialog is displayed 3 Enter d Setup exe and then click OK Replace d with the drive letter for your CD The InstallShiel...

Page 55: ...tall 6 Select the required product s and then enter the License Number s as found on the Software License Certificate You must enter the correct license number s to proceed with the installation If yo...

Page 56: ...ting Started Guide 7 Click Next The Ready to Install the Program page is displayed 8 Click Install to begin installing Spectrum The Installing Spectrum Software page is displayed which informs you of...

Page 57: ...allation Disk LX108925 into your CD drive 11 If the installation program does not start automatically from the Start menu select Run The Run dialog is displayed 12 Enter d setup exe and then click OK...

Page 58: ...Series Getting Started Guide 13 Click Next The License Agreement page is displayed 14 Read the license and if you accept the terms select that option and then click Next The Ready to Install the Progr...

Page 59: ...ment The Installing Raman Instrument page is briefly displayed During this installation the DirectX Setup page is displayed 16 Read the license and if you accept the terms click Yes The Installing Ram...

Page 60: ...age 18 Click Start The Enter Instrument Details page is displayed 19 In the Instrument Alias field enter a name for your Raman instrument This should be a unique name to identify this instrument 20 In...

Page 61: ...for the PerkinElmer software that is already installed on the PC The default User name and Password are administrator The Locate Instrument Configuration Files page is displayed 22 Place your Raman I...

Page 62: ...ng the supplied USB 2 0 cable L1320821 Figure 21 USB 2 0 cable NOTE If you disconnect the instrument control USB cable and reattach it to a different USB port then you will be prompted for the drivers...

Page 63: ...s helping you install software for USB Device In this case continue with the installation in the same way as for IDUS 26 Select Install from a list or specific location Advanced and then click Next Th...

Page 64: ...28 Click Next The Completing the Found New Hardware Wizard is displayed 29 Click Finish The Found New Hardware Wizard closes 30 On the Install PerkinElmer Raman Instrument page click Continue The Ins...

Page 65: ...nt Installer Information dialog is displayed 33 At this point Click No Follow the steps described below to continue the installation procedure and install the alignment camera 34 Plug the USB cable fr...

Page 66: ...l the Videology USB2 0 Viewer navigate to C Program Files PerkinElmer ServiceIR Raman and double click on Autorun exe The Welcome to Videology USB2 0 Camera Installation Wizard page is displayed 37 Cl...

Page 67: ...Next The Your Camera is Successfully Installed page is displayed 39 Click Finish The Videology USB2 0 Camera Software Setup page is displayed 40 Click Install Viewer The Welcome to the InstallShield...

Page 68: ...ck Next The Choose Destination Location page is displayed 42 If required click Change and navigate to your chosen destination folder and then click Next The Ready to Install the Program page is displa...

Page 69: ...tic probe drivers as described in the Raman Triggered Fiber Optic Probe leaflet L1321887 and then connect the fiber optic cables from the triggered fiber optic probe to the RamanStation 400 as describ...

Page 70: ...the center of possible travel in the X and Y axes and at its lowest position in the Z axis The front and right sides of the stage will be aligned 3 Close Spectrum software 4 Remove the alignment camer...

Page 71: ...Introduction to the RamanStation 400...

Page 72: ...that the laser power is on Active This LED indicates the status of the laser shutters If the LED is illuminated then the shutters are activated open and the laser radiation will be present throughout...

Page 73: ...anStation 400 73 Sample Compartment To open the sample compartment lift the cover using the handle at the front of the RamanStation 400 as shown in Figure 23 Figure 23 Opening the sample compartment o...

Page 74: ...tment Figure 24 Figure 24 RamanStation 400 sample compartment The Versatile Sample Holder L1320227 is supplied with your RamanStation 400 Figure 25 You can use this holder to collect data from microsc...

Page 75: ...00F instrument you can collect data using a fiber optic probe see Fiber Optic Probe on page 77 for more details which enables the remote measurement of samples The RamanStation 400F instrument can als...

Page 76: ...76 RamanStation 400 Series Getting Started Guide...

Page 77: ...Fiber Optic Probe...

Page 78: ...r details of how to use a triggered fiber optic probe see the Raman Triggered Fiber Optic Probe leaflet L1321887 Please read and understand the following general safety precautions before proceeding I...

Page 79: ...verified with PerkinElmer prior to use The probe body contains the filtering optics and is interfaced with two fiber optic cables an excitation fiber and collection fiber Care should be taken when han...

Page 80: ...move the instrument to permit easier access to the rear panel 4 Remove the fiber port cover assembly if fitted see Attaching the Fiber Port Cover Assembly on page 49 5 Remove the retained screw in cap...

Page 81: ...to the rear panel 4 Remove the plug in the socket marked LED FIBER 1 5 Carefully remove the retained ends of the FOP by unscrewing and pulling the cables away from the couplers perpendicularly Be ext...

Page 82: ...ar infrared region Room lights and sunlight will contaminate your spectra Take care not to misinterpret room lights or sunlight features as Raman features Sampling Liquids Liquid samples can be analyz...

Page 83: ...rescence is a problem and it is not possible to use quartz containers then it is possible to acquire a spectrum of the container and spectrally subtract this from any spectra containing this fluoresce...

Page 84: ...84 RamanStation 400 Series Getting Started Guide...

Page 85: ...Using the RamanStation 400...

Page 86: ...number of tasks The procedures provided may not be the most straightforward or simple for the task you wish to perform so treat these Standard Operating Procedures SOPs as a starting point and with ti...

Page 87: ...ched on 5 To turn on the spectrometer press the PSU power switch ON Figure 29 ON OFF Figure 29 The PSU power switch 6 Check that the Power LED on the instrument keypad is On The Laser LED and the Acti...

Page 88: ...Switch off the spectrometer by pressing the power switch on the PSU to OFF ON OFF 3 If appropriate remove the stage controller power supply from the mains outlet 4 If appropriate ensure that all sampl...

Page 89: ...al parameters are stored as REX files This is the Raman Experiment file If you would like to save this set of parameters as the default set that loads when the software is opened then save your experi...

Page 90: ...nt page Advanced tab Yes Sampling Area Experiment page Sampling tab Select the number and style of sampling points Sampling Type Experiment page Sampling tab If applicable select the spacing and numbe...

Page 91: ...n which the scan data will be stored NOTE If you choose to save spectra to a folder other than the default one specified for your instrument the data will not be displayed automatically To view the sp...

Page 92: ...and not to the Auxiliary Control port 1 Open the sample compartment lid and ensure that there is no sample holder loaded in the sample accessory stage 2 Close the sample compartment lid 3 Select Setu...

Page 93: ...ints towards the cuvette holder mount points to the left side of the instrument To do this unscrew the lens snout from the stage as shown below in Figure 30 Figure 30 Unscrew the lens snout and rotate...

Page 94: ...ut deliberate intervention due to the dual redundant interlock system installed 1 Follow SOP 1 to turn the instrument on 2 Load the appropriate sample holder onto the XYZ sample accessory stage SOP 5...

Page 95: ...can open the sample compartment lid while in Spectral Preview mode If you do so data collection will stop but the motors on the sample accessory will still function Data acquisition will continue whe...

Page 96: ...log is displayed 3 Click 4 In the Accessory Configuration section select Fiber Optic Probe from the Beam Path drop down list The following warning message is displayed 5 Ensure that laser safety preca...

Page 97: ...lect Setup from the Instrument menu in Spectrum The Scan and Instrument Setup dialog is displayed 3 Click Spectral Preview Real time spectral acquisition will commence and spectra are displayed in the...

Page 98: ...environment or following a service repair of the instrument or accessories NOTE The sample compartment lid must be kept closed during all stages of calibration Backgrounds are always collected during...

Page 99: ...the instrument has been brought back within specification If the wavelength calibration verification fails because the spectral intensity is low then this may also be due to the calibration sample ho...

Page 100: ...ion tab Figure 33 The Validation Calibration dialog 7 Select Wavelength Calibration Verification and then click OK The wavelength calibration verification routine begins This will take a few minutes A...

Page 101: ...Select Wavelength X axis and then click OK The instrument will now perform the wavelength calibration routine This will take some time After completing the wavelength calibration routine a report is g...

Page 102: ...andard for Raman spectroscopy when using 785 nm excitation Intensity calibration should typically be performed on an annual basis that is during an annual instrument service or whenever the instrument...

Page 103: ...epeat the calibration 7 Remove the intensity correction standard and store safely Any data acquired will now be intensity corrected Remember that if you have a fiber optic probe you must intensity cal...

Page 104: ...et Holder Kit for Motorized Stage Contains tablet holder baseplate sample discs tablet holder cups and tweezers L1245025 Custom Tablet Molding Kit L1321675 Multiwell Plate Holder for Motorized Microsc...

Page 105: ...ture 500 C maximum working pressure 3000 psi 5 m cabling L1320013 Purge Jacket for Raman probe max required to enable max probe operation at temperatures between 200 C and 500 C L1320030 Triggered Fib...

Page 106: ...106 RamanStation 400 Series Getting Started Guide...

Page 107: ...Maintenance and Troubleshooting...

Page 108: ...immediately If anything that is spilled enters the main body of the spectrometer make the instrument inoperative and then contact a PerkinElmer Service Engineer WARNING Do not directly clean electrica...

Page 109: ...ing maintenance or servicing of the FOP The immersion sleeve if purchased is manufactured with 316 stainless steel or Hastelloy C and contains either a quartz or sapphire optical window that is sealed...

Page 110: ...not recognized Description This may mean that there is not enough power on the USB sockets on your PC Solution Ensure that your PC has USB 2 0 sockets If you are not sure use a USB 2 0 power hub Prob...

Page 111: ...B Device In this case continue with the installation in the same way as for IDUS 2 Select Install from a list or specific location Advanced and then click Next The Please choose your search and instal...

Page 112: ...This means that for some reason the motorized stage is jammed This typically happens if the sample holder on the stage is different to that selected on the Sampling tab in the Scan and Instrument Set...

Page 113: ...lected Description This means that the camera is inverted If this is not corrected you will not be able to use Image Survey Solution In Video Preview select Invert Image from the Camera menu The cross...

Page 114: ...u are unsure how to do this please refer to SOP 1 Problem You get any of the following messages An unexpected error has occurred EAccess Violation or Divide by Zero Description While working in the so...

Page 115: ...ion of Raman Instrument Control before installing a new version for example if you have purchased an accessory that requires a later version of Raman Instrument Control Solution To uninstall Raman Ins...

Page 116: ...from contamination The customer s responsible body is required to follow the Equipment Decontamination Procedure and complete the Certificate of Decontamination These documents are available on the Pe...

Page 117: ...most effective means of protecting the customer s environment The requirements for waste collection reuse recycling and recovery programs are set by the regulatory authority in your location Contact...

Page 118: ...118 RamanStation 400 Series Getting Started Guide...

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