PerkinElmer RamanStation 400, Getting Started Manual

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Using the RamanStation 400 .
101
SOP-11: Wavelength Calibration of the
RamanStation 400
Wavelength calibration should only be performed if the instrument has failed a wavelength
calibration verification.
1. Ensure the RamanStation 400 is switched on.
If not follow SOP-1.
2. Attach the external neon calibration accessory (L1320220) to the rear panel.
Push the large connector into the socket labeled CAL ASSY, and carefully connect the
small cable to the COL coupler .
3. Select Validate and then Instrument from the Instrument menu in Spectrum.
The Validation / Calibration dialog is displayed.
4. Select the Calibration tab.
Figure 34 The Validation / Calibration dialog
5. Select Wavelength (X-axis), and then click OK.
The instrument will now perform the wavelength calibration routine. This will take some
time.
After completing the wavelength calibration routine, a report is generated on-screen which
describes the result of the wavelength calibration.
The instrument is now wavelength calibrated.
SOP-12: Laser Frequency Calibration of the
RamanStation 400
Laser frequency calibration should only be performed if the instrument has failed a
wavelength calibration verification.
1. Ensure the RamanStation 400 is switched on by following SOP-1.
2. Open the Scan and Instrument Setup dialog and on the Hardware tab ensure that the
laser power is set to 100%.
3. Click Spectral Preview.
4. Focus on the polystyrene calibration sample.
When the polystyrene sample is optimally focused, close the Scan and Instrument Setup
dialog by clicking Exit.