PerkinElmer RamanStation 400, Getting Started Manual

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100 . RamanStation 400 Series Getting Started Guide
SOP-10: Wavelength Calibration Verification of the
RamanStation 400
1. Ensure the instrument is switched on by following SOP-1.
2. Open the Scan and Instrument Setup dialog, and on the Hardware tab ensure that the
laser power is set to 100%.
3. Focus on a sample of polystyrene using the autofocus buttons and Spectral Preview.
4. When the polystyrene sample is optimally focused, close the Scan and Instrument Setup
dialog by clicking Exit.
5. Select Validate and then Instrument from the Instrument menu in Spectrum.
The Validation / Calibration dialog is displayed.
6. Select the Verification tab.
Figure 33 The Validation / Calibration dialog
7. Select Wavelength Calibration Verification, and then click OK.
The wavelength calibration verification routine begins. This will take a few minutes.
After completing the wavelength calibration verification routine, an audit log is
generated.
8. Remove the polystyrene sample and store safely.
The instrument is now ready for use.
In the unlikely event that the wavelength calibration verification should fail, follow Figure 32.
If the RamanStation 400 wavelength calibration verification fails for the sample
compartment, this does not necessarily mean that the performance of the fiber optic probe is
also affected.
Further details on the wavelength calibration verification are provided as a spectral (*.sp)
file, which is saved in
C:\pel_data\config\Raman\Calibration Files\Logs
folder.