PerkinElmer RamanStation 400, Getting Started Manual

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Using the RamanStation 400 .
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SOP-8: Sample Positioning when using a Fiber Optic
Probe
You can use the Spectral Preview button to position your sample for optimum spectral
collection efficiency. Sample positioning and alignment in preview mode allows you to
position and focus on a specific area and to collect spectral data from that location. It can
also be used to enhance the signal-to-noise ratio of spectral data. The data that is displayed
on screen during a spectral preview cannot be saved.
1. Ensure that the RamanStation 400 is switched on.
If not, follow SOP-1.
2. Select Setup from the Instrument menu in Spectrum.
The Scan and Instrument Setup dialog is displayed.
3. Click Spectral Preview.
Real time spectral acquisition will commence and spectra are displayed in the spectral
preview tab.
4. Set the Preview Scan time to 1 second, and then press Enter.
This will automatically acquire another background. You can enter a Preview Scan time
in the range 0.1 – 10 seconds.
The sample may now be positioned and aligned correctly for optimized data collection.
The spectral data can be optimized by adjusting the distance between the Fiber Optic
Probe and the sample.
5. Use the Pause and Play buttons to examine the spectrum in the Spectral Preview panel.
When you click Play the preview acquisition resumes.
You can now collect and save spectral data.