10
Using the Tali
™
Image-Based Cytometer
Workflow
The Tali
™
Image-Based Cytometer captures a series of images (i.e., fields of view) of
the sample in Tali
™
Cellular Analysis Slides and uses sophisticated digital image
analysis algorithms to perform assays for cells in suspension. All Tali
™
Assays share
the same basic workflow and user interface, but differ in which channels the images
are captured and which algorithm is used for analysis. The table below describes the
major steps for using the Tali
™
Image-Based Cytometer.
Step
Action
Page
1
Optional
: Adjust global counting settings
2
Select the Tali
™
Assay you wish to perform
3
Load your sample.
4
Focus the field of view
5
Optional
: Perform a new background measurement or select a
previously recorded background (not applicable to the Quick
Count Assay)
6
Capture images of the sample
7
Review captured images
8
Set gates and thresholds
9
Annotate and export your data
Before using the Tali
™
Image-Based Cytometer for the first time, you need to align
the cameras and calibrate the instrument using the calibration beads provided with
the instrument. See page 32 for more information.
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