16
Load Sample
Load the Tali
™
Cellular Analysis
Slide
The Tali
™
Cellular Analysis Slides are plastic, disposable slides that hold the sample
in two separate, enclosed chambers (A and B) that allow the analysis two different
samples or replicates of the same sample. Each chamber has a 25-μL sample
capacity. Follow the guidelines below to load your samples into the Tali
™
Cellular
Analysis Slides.
Do
not
touch the optical surfaces of the Tali
™
Cellular Analysis Slides. Hold the
slides by the edges.
Use 25 μL of sample volume per slide chamber. Do not overfill or underfill the
slide chambers.
Pipet the sample at an angle of approximately 80° into the half moon-shaped
sample loading area (see figure below). The sample is loaded into the chamber
through capillary action.
Take care to avoid forming bubbles in the sample or to cause back splatter.
The Tali
™
Image-Based Cytometer counts the cells in the chamber opposite the
loading direction.
For example, to count the sample in Chamber A, you need to insert the Tali
™
Cellular Analysis Slide into the instrument with the Chamber B-side first (see
figure below). For inserting the Tali
™
Cellular Analysis Slide into the
Important
Tali
™
Cellular Analysis Slides are specially designed for use with the Tali
™
Image-
Based Cytometer exclusively. Use of other slides will result in inaccurate cell counts
and can damage the Tali
™
Image-Based Cytometer. See page 39 for ordering
information.
The slide port button, located on the top right side of the touch screen, is
used only for opening the slide port to eject the Tali
™
Cellular Analysis
Slide in case of an error. Pressing this button will not insert the slide into
the instrument or eject it after a run is completed.
Continued on next page
