34
Calibrate the Tali
™
Image-Based Cytometer
Introduction
Calibrating the green and red fluorescent channels of the Tali
™
Image-Based
Cytometer sets the dynamic range of the instrument. The calibration is most
effective when performed after the alignment sequence.
Materials needed
Tali
™
Calibration Beads (Cat. no. T10790) (supplied with the instrument or available
separately; see page 39)
Note:
Tali
™
Calibration Beads contain separate vials of green and red beads necessary for
calibrating the green and red channels of the Tali
™
Image-Based Cytometer.
1.
Touch
Calibrate GFP/RFP
on the Settings screen. The
Calibration Dialog Box opens.
Calibrate the green
and red channels
2.
Touch
OK
to proceed with calibration. Green channel calibration dialog box is
displayed.
3.
Load 25 μL of Tali
™
Green Calibration Beads into the Tali
™
Cellular Analysis
Slide as described on page 16 and insert the slide into the slide port.
4.
Touch
Continue
; the slide will automatically be pulled into the instrument and
green channel calibration will be initiated.
Continued on next page
