Life technologies Invitrogen Tali User Manual Download Page 42

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Headquarters

5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone +1 760 603 7200 | Toll Free in USA 800 955 6288

For support visit

www.invitrogen.com/support or email [email protected]

www.lifetechnologies.com

Summary of Contents for Invitrogen Tali

Page 1: ...user guide Tali Image Based Cytometer Catalog Number T10796 Publication Part Number MAN0003766 Revision Date 28 April 2011 ...

Page 2: ...y 15 Load Sample 16 Focus Image 18 Measure the Background optional 19 Run Sample 20 Analyze Data 25 Export Data 28 Maintenance 31 Update the Tali Image Based Cytometer Firmware 31 Adjust the Camera Alignment 32 Calibrate the Tali Image Based Cytometer 34 Clean the Tali Image Based Cytometer 36 Appendix 37 Safety Information 37 Accessory Products 39 Technical Support 40 Purchaser Notification 41 ...

Page 3: ...he warranty does not cover in transit damage See page 11 for instructions on installing the Tali Image Based Cytometer Registering your instrument Visit www invitrogen com tali to register your instrument You will be asked to supply the serial number your name and your contact details Registering your instrument ensures that you will receive notifications of software upgrades and information on ne...

Page 4: ...xpressing GFP and RFP The Tali Image Based Cytometer offers an intuitive user interface and provides the option to save data and generate a report which can then be transferred to a PC using the USB drive supplied with the instrument or available separately The Tali Image Based Cytometer is supplied with disposable Tali Cellular Analysis Slides see page 8 which are also available separately see pa...

Page 5: ... sample and presents the relevant data e g cell count vs cell size cell count vs fluorescence in tables and histograms The data from the analysis including the image files can be downloaded to a USB flash drive immediately after the assay and transferred to a computer for sample comparisons Viability determines the number and proportion of viable and dead cells using the Tali Viability Kit Dead Ce...

Page 6: ... insert the Tali Cellular Analysis Slide containing the sample into the instrument for analysis USB Port The USB port allows you to transfer and save the cell count data and images to your computer for record keeping and printing purposes The USB drive supplied with the instrument or any other standard USB drive is inserted into the USB port for data transfer See page 29 for instructions on export...

Page 7: ...shown below Power Inlet The power inlet connects the instrument to an electrical outlet through the supplied power cord and the appropriate plug based on the electrical outlet configuration in your country On Off Switch The on off switch is located above the power inlet and is as the main power switch It is not necessary to use the on off switch for day to day operation of the instrument Continued...

Page 8: ...lular Analysis Slides The Tali Cellular Analysis Slides are disposable slides composed of polylactic acid PLA a low fluorescence plastic Each slide holds the sample in two separate enclosed chambers A and B allowing the analysis of two different samples or replicates of the same sample Each chamber has a 25 μL sample capacity The Tali Image Based Cytometer captures a series of images i e fields of...

Page 9: ...of fields captured Sample concentration range 1 105 1 107 cells mL Recommended sample diameter range 5 60 μm Required sample volume 25 μL Firmware Tali Image Based Cytometer Firmware visit www invitrogen com tali for updates USB Drive 4 Gigabyte Optics Optics 3 channels bright field green fluorescence red fluorescence Excitation Green channel LED 458 20 nm Red channel LED 530 20 nm Filters Green c...

Page 10: ...he Tali Image Based Cytometer Step Action Page 1 Optional Adjust global counting settings 13 2 Select the Tali Assay you wish to perform 15 3 Load your sample 16 4 Focus the field of view 18 5 Optional Perform a new background measurement or select a previously recorded background not applicable to the Quick Count Assay 19 6 Capture images of the sample 20 7 Review captured images 21 8 Set gates a...

Page 11: ...rical outlet Be sure to use only the power cord supplied with your instrument Powering the instrument with an unapproved power cord may damage the instrument 4 Turn on the main power switch i e On Off switch located above the power inlet port see image on page 7 5 When you are ready to use turn on the Tali Image Based Cytometer by pressing the Power button on the front of the instrument Home scree...

Page 12: ...ime The date and time is already preset when you receive the instrument To reset the date and time use the Date and Time roller wheels that respond to the movement of a finger across the screen as if they were wheels 1 Press Settings on the touch screen the Settings screen is displayed 2 Select the date and time by bringing the desired value to the center position on the roller wheel located under...

Page 13: ...jects to include in the measurement based on their roundness Increasing the value for circularity requires objects to be rounder for inclusion in the measurement and may be useful if you need to distinguish perfectly round cells from more oddly shaped cells Decreasing the circularity allows objects to be less round for inclusion in the measurement and may be useful if the cell type is not particul...

Page 14: ...alibrate the Tali Image Based Cytometer after updating the firmware or when using assay reagents not formulated for the instrument To recalibrate the Tali Image Based Cytometer see page 32 The internal working memory of the Tali Image Based Cytometer is 145 Gigabytes sufficient for storing comprehensive data from 1000 sample runs However we recommend that you save your data to the USB drive after ...

Page 15: ...a numeric keyboard pop up screen is displayed 3 Using the keyboard type the name of the sample series using up to 40 alpha numeric characters and then press Save Each sample run in the series will be appended with a number to reflect the order in which it was run To return to the previous screen without assigning a name to the sample series press Close Note If you select Name later the instrument ...

Page 16: ...re to avoid forming bubbles in the sample or to cause back splatter The Tali Image Based Cytometer counts the cells in the chamber opposite the loading direction For example to count the sample in Chamber A you need to insert the Tali Cellular Analysis Slide into the instrument with the Chamber B side first see figure below For inserting the Tali Cellular Analysis Slide into the instrument see pag...

Page 17: ...g direction For example to count the sample in Chamber A insert the Tali Cellular Analysis Slide into the slide port of the instrument with the Chamber B side first until it stops see figure below Do not forcefully push the slide any further 3 Touch Press to insert new sample the slide will automatically be pulled into the instrument Note If you are performing a background measurement the button f...

Page 18: ...instrument 2 Touch Zoom to review the image Correctly focused images have uniformly dark colored cells surrounded by bright halos Cells may be undercounted when the transition between the background and the edges of the cells are fuzzy and the cells have undefined boundaries Cells maybe overcounted when they have bright centers and dark perimeters ...

Page 19: ...n a fluorescence threshold for you which you can change manually after performing your assay If you have already measured the background the dropdown menu will display the latest background measurement on file 2 To use a previously obtained background measurement select the file name of the measurement from the In use drop down menu To perform a new background measurement load the Tali Cellular An...

Page 20: ...sed Cytometer automatically ejects the slide and provides the data from the analysis of the captured images in the Sample tab The analysis includes information about the average cell size the number of cells counted total cell concentration as well assay specific data and histogram plots Note In this example the Tali Apoptosis Assay specific data include the concentration percentage and number of ...

Page 21: ... Review captured images 3 To review a different section of an image viewed at 4X or 16X magnification touch the image to show the navigation tool 4 Touch the appropriate section on navigation tool to display the desired section Touch the image outside its boundaries to hide the navigation tool to hide and review the image Note The navigation tool is only available for reviewing the images at 4X an...

Page 22: ...he same buttons would be labeled SYTOX Blue and RFP if the RFP Viability assay was selected 2 Touch appropriate button on the Layers tab to display the image captured in a particular channel in this example touch Annexin V or PI Press the same button again to remove the layer from the display Note You may superimpose layers by touching multiple layer buttons 3 To identify the cells counted in a pa...

Page 23: ...For example the histograms are labeled Cell size Annexin V and PI for the Tali Apoptosis assay The thin blue lines on the thumbnails histograms depict the set thresholds 2 The pop up window displays the Cell size vs of cells histogram and the slider bar for adjusting the lower and upper boundaries for cell size 3 Move the two blue buttons on the slider bar horizontally to set the lower and upper b...

Page 24: ... confirm and return to the previous screen Only cells above the threshold i e to the right of the blue boundary line are counted and the data is updated with the new count To return to the previous screen without setting the threshold for cell size touch Close 9 To set the threshold for PI the remaining fluorescent channel touch PI and repeat the same procedure for PI Biological molecules found wi...

Page 25: ...ing the run as jpg files or the final report containing aggregate data and calculations as a pdf file Select data files 1 Touch Data to navigate to the Data screen which displays the stored data files in a list on the right side of the screen The most recent data file is displayed in the first line of the list 2 Drag the red button on the scrollbar vertically to move up and down the list to access...

Page 26: ...le name containing up to 40 alpha numeric characters using the touch screen keyboard and then touch Save Touch Close to return to the previous screen without renaming the file Delete data files 1 Select the data file s to delete by touching the corresponding line of the file list The selected line s will be highlighted Touch Select all to highlight all files To deselect touch the highlighted file ...

Page 27: ... 1 To attach a note to your data file select the file to annotate and then touch the Notes box above the Data window to display the alpha numeric keyboard pop up screen 2 Enter your comments using the touch screen keyboard and then touch Save Your comments in the Notes box will be attached to the file and shown on all the Data tabs i e Layers Zoom and Analysis To return to the previous screen with...

Page 28: ...ual particle cell in the sample You can import the csv file into any spreadsheet program Choosing Images exports a single image in each channel collected for each field of view For example if you choose Images for an Apoptosis assay with 9 fields of view the instrument will export a bright field image a green fluorescence image and a red fluorescence image for each field of view Images are exporte...

Page 29: ...e file list The selected line will be highlighted Note Touch Select all to highlight all files To deselect a file touch the highlighted line on the file list again 3 Touch Data table csv Images jpg or Report pdf to export the data in the designated formats To export data in all these formats touch All formats 4 Transfer the files on the USB drive to your PC You may open the exported files using th...

Page 30: ...30 Export Data continued Cell analysis report The image below shows an example of a cell analysis report exported as a pdf file ...

Page 31: ...ware file to the root menu of your USB drive Note Only one version of firmware should be on the USB drive at a time 3 Touch Update firmware on the Settings screen The Update Dialog Screen is displayed Update the firmware 4 Insert the USB drive into the USB port of the Tali Image Based Cytometer and touch Update Note A green dot on the Update button indicates that the instrument recognizes the USB ...

Page 32: ...ibration Beads Cat no T10790 supplied with the instrument or available separately see page 37 1 Load 25 μL of Tali Alignment Beads into the Tali Cellular Analysis Slide as described on page 16 2 From the Home screen select Quick count 3 Insert the Tali Cellular Analysis Slide into the slide port of the instrument as described on page 17 and touch Press to insert new sample the slide will automatic...

Page 33: ...he alignment has been processed use the directional keys to position the green circles over the corresponding Tali Alignment Bead 9 Touch Set alignment to confirm the correct alignment of the cameras To go back to the previous screen without completing the alignment touch Cancel ...

Page 34: ...ate vials of green and red beads necessary for calibrating the green and red channels of the Tali Image Based Cytometer 1 Touch Calibrate GFP RFP on the Settings screen The Calibration Dialog Box opens Calibrate the green and red channels 2 Touch OK to proceed with calibration Green channel calibration dialog box is displayed 3 Load 25 μL of Tali Green Calibration Beads into the Tali Cellular Anal...

Page 35: ...e ejected and the Red channel calibration dialog box will be displayed 6 Load 25 μL of Tali Red Calibration Beads into the Tali Cellular Analysis Slide insert the slide into the slide port and touch Continue to initiate red channel calibration 7 After the calibration process is completed touch OK to return to the Settings screen There is no need to recalibrate each time the instrument is turned on...

Page 36: ...e power button the power inlet the slide port or the USB ports Never pour or spray any liquids directly on the instrument to avoid electrical shock when the instrument is plugged in Do not use abrasive cleaning solutions or material to prevent the touch screen from getting scratched Clean the instrument case Wipe the instrument case of the Tali Image Based Cytometer using a soft lint free cloth mo...

Page 37: ... Do not install the instrument on a slant or a place prone to vibrations which induces the risk of instrument malfunction or damage of the instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet and AC adapter To avoid potential shock hazard make sure that ...

Page 38: ... product conforms to UL61010 1 CSA C22 2 No 61010 1 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part I General Requirements Instruments bearing the TUV symbol are certified by TUV Product Services to be in conformance with the applicable safety standards for the US and Canada FCC compliance This equipment has been tested and found to comply with the limi...

Page 39: ...on Beads includes 1 tube each of Tali Green Calibration Beads Tali Red Calibration Beads and Tali Alignment Beads 1 kit T10790 Tali Cellular Analysis Slides 50 slides 500 slides T10794 T10795 Tali Image Based Cytometer Assays The assay kits listed below have been designed and optimized for use with the Tali Image Based Cytometer For more information refer www invitrogen com or contact Technical Su...

Page 40: ...603 7200 Tel Toll Free 1 800 955 6288 Fax 1 760 602 6500 E mail techsupport invitrogen com Japanese Headquarters LOOP X Bldg 6F 3 9 15 Kaigan Minato ku Tokyo 108 0022 Tel 81 3 5730 6509 Fax 81 3 5730 6519 E mail jpinfo invitrogen com European Headquarters Inchinnan Business Park 3 Fountain Drive Paisley PA4 9RF UK Tel 44 0 141 814 6100 Tech Fax 44 0 141 814 6117 E mail eurotech invitrogen com SDS ...

Page 41: ...ver an error in any of our publications please report it to our Technical Support Representatives Life Technologies Corporation shall have no responsibility or liability for any special incidental indirect or consequential loss or damage whatsoever The above limited warranty is sole and exclusive No other warranty is made whether expressed or implied including any warranty of merchantability or fi...

Page 42: ...quarters 5791 Van Allen Way Carlsbad CA 92008 USA Phone 1 760 603 7200 Toll Free in USA 800 955 6288 For support visit www invitrogen com support or email techsupport invitrogen com www lifetechnologies com ...

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