20
Run Sample
1.
To specify the number of fields of
view to capture, touch the
arrow
button
on the
# of images to capture
drop-down menu and select from
the available options.
Capture images
2.
To capture the specified number of fields of view, touch
Press to run sample
.
While the image capture is in progress, an ongoing update is provided by the
progress bar.
3.
After capturing the specified number of fields of view, the Tali
™
Image-Based
Cytometer automatically ejects the slide and provides the data from the analysis
of the captured images in the Sample tab. The analysis includes information
about the average cell size, the number of cells counted, total cell concentration,
as well assay-specific data and histogram plots.
Note:
In this example, the Tali
™
Apoptosis
Assay-specific data include the
concentration, percentage, and number of
live, dead, and apoptotic cells, as well as
the histograms for cell size and
fluorescence intensity for Annexin V and
propidium iodide (PI).
4.
To run the next sample, insert the new slide, and repeat the procedure.
Continued on next page
