32
Adjust the Camera Alignment
Introduction
The proper alignment of the two cameras inside the Tali
™
Image-Based Cytometer
is essential for obtaining the most accurate data from the instrument. Follow the
protocol below to align the cameras so that the fluorescent image is properly
overlaid with the bright field image. After unpacking the Tali
™
Image-Based
Cytometer, perform the alignment procedure using the Tali
™
Alignment Beads
before calibrating the instrument or running any samples for analysis. Check the
alignment of the Tali
™
Image-Based Cytometer every 90 days.
Materials needed
Tali
™
Alignment Beads, included in Tali
™
Calibration Beads (Cat. no. T10790)
(supplied with the instrument or available separately; see page 37)
1.
Load 25 μL of Tali
™
Alignment Beads into the Tali
™
Cellular Analysis Slide as
described on page 16.
2.
From the Home screen, select
Quick count
.
3.
Insert the Tali
™
Cellular Analysis Slide into the slide port of the instrument as
described on page 17 and touch
Press to insert new sample
; the slide will
automatically be pulled into the instrument.
4.
Focus the Tali
™
Alignment Beads using the image adjustment (focus) knob on
the right side of the instrument as described on page 18.
5.
Touch
Press to run sample
.
6.
After the count is completed, go to the Settings screen
and touch
Align cameras
.
Align the cameras
7.
Using the same slide and focus, initiate the alignment procedure following the
directions given on the screen.
Continued on next page
