Life technologies Invitrogen Tali, User Manual

Page: 14 / 42

14
Guidelines for Performing Tali ™ Assays
General guidelines
To obtain the best results, follow these recommendations:

Wear gloves during sample handling.

Do not touch the optical surfaces of the Tali
™
Cellular Analysis Slides. Hold the
slides by the edges. See page 16 for guidelines on loading the Tali
™
Cellular
Analysis Slides.

Use the Tali ™ Image-Based Cytometer at room temperature only (10–35ºC).

The Tali
™
Image-Based Cytometer contains delicate optics. Place the instrument
on a flat, dry surface that is free from excessive vibration. Do not spill liquids on
the surface of the instrument or introduce liquids into its interior.

The Tali
™
Image-Based Cytometer can accurately count cells 5 μm to 60 μm in
diameter.

The recommended sample concentration range for the Tali
™
Image-Based
Cytometer is 1 × 10
5
to 1 × 10
7
cells/mL; however, the sample concentration
does not need to be exact to perform an assay.

For accurate viability count results, ensure the counting area is covered with cell
suspension and count cells immediately after staining per the assay protocol.

Re-calibrate the Tali
™
Image-Based Cytometer after updating the firmware or
when using assay reagents not formulated for the instrument. To recalibrate the
Tali
™
Image-Based Cytometer, see page 32.

The internal working memory of the Tali
™
Image-Based Cytometer is
145 Gigabytes, sufficient for storing comprehensive data from 1000 sample runs.
However, we recommend that you save your data to the USB drive after
completing each experiment. Using the USB drive, you may then transfer the
data to your PC as described in
Export Data (page 25).

After using Tali ™ Cellular Analysis Slides, appropriately dispose of them as
biohazardous waste. Do not reuse the Tali
™
Cellular Analysis Slides.

Turn off the Tali
™
Image-Based Cytometer at the end of the day.
Accuracy vs. assay
speed
The Tali
™
Image-Based Cytometer performs counts by simultaneously capturing
bright field and fluorescent images of the sample in the Tali
™
Cellular Analysis Slide
and then analyzing the captured fields of view using assay-specific algorithms.

You can obtain higher accuracy by capturing more images, and thus analyzing
more cells.

You can obtain faster assay speeds by analyzing fewer fields of view.

You can calculate the theoretical CV using the following equation:
 
n n
where n is the number of cells counted.