Infors HT Labfors 5, Operating Manual

Page: 7 / 208

Labfors 5 – Version for Microorganisms
Table of Contents
22 January 2019 Page 5 of 205
8.1.13 Equipping Port with Septum Collar and
Septum for Inoculation ............................... 116
8.1.14 Mounting and Preparing the Exit Gas Cooler
.................................................................... 117
8.1.15 Mounting the Cold Finger ........................... 119
8.1.16 Checking Lubrication of the Mechanical Seal
.................................................................... 119
8.1.17 Preparing the Sensors ................................ 119
8.1.17.1 Calibrating the pH Sensor ........... 120
8.1.17.2 Mouting a Sensor into a 12 mm
Port .............................................. 121
8.1.17.3 Mounting Sensors with Electrode
Holder .......................................... 121
8.1.17.4 Mounting the Antifoam Sensor.... 124
8.1.18 Preparing the Super Safe Sampler ............ 127
8.1.19 Mounting the Sparger Hose and the Inlet Air
Filter ............................................................ 128
8.1.20 Preparing the Gassing Hose Line on the Basic
Unit ............................................................. 129
8.1.21 Calibrating the Pumps ................................ 129
8.1.22 Preparing the Reagent Bottles, Pumps and
Hoses ......................................................... 130
8.1.23 Sterile Hose Connections ........................... 133
8.1.24 Removing the Pump Heads ....................... 133
8.1.25 Checklist Before Autoclaving ...................... 134
8.1.26 Autoclaving ................................................. 135
8.2 Connecting the Culture Vessel and Preparing the
Cultivation .................................................................. 137
8.2.1 Connecting a Single Walled Vessel ........... 137
8.2.2 Connecting a Double Walled Culture Vessel
.................................................................... 139
8.2.3 Mounting the Pump Heads ......................... 140
8.2.4 Filling the Reagent Hoses .......................... 140
8.2.5 Connecting the Gassing ............................. 141
8.2.6 Connecting the Exit Gas Cooler ................. 142
8.2.7 Coupling the Motor ..................................... 143
8.2.8 Filling the Culture Vessel ............................ 143
8.2.9 Inserting the Temperature Sensor (Pt100) into
the Pocket................................................... 144
8.2.10 Connecting the Antifoam Sensor................ 144
8.2.11 Connecting the pH Sensor ......................... 145
8.2.12 Connecting the pO
2
Sensor ........................ 146
8.2.13 Polarising the pO
2
Sensor (METTLER) ...... 147
8.2.14 Calibrating the pO
2
Sensor ......................... 147