Labfors 5 – Version for Microorganisms
Before Fermentation/Cultivation
Page
106
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22 January 2019
8 Before Fermentation/Cultivation
The following chapters describe all the preparatory work before
starting the fermentation/cultivation process. This essentially com-
prises:
Preparing and autoclaving the culture vessel:
Checking the gaskets (O-rings) on component parts and
culture vessel
Mounting component parts
Filling or moistening the culture vessel
Preparing sensors and other accessories
Autoclaving
Connecting the culture vessel and preparing for cultivation:
Connecting the cables and hoses between the culture
vessel and the basic unit
Filling the vessel if necessary
Preparing sensors and other accessories
8.1 Preparing and Autoclaving the Culture Vessel
All accessories required for later cultivation must be prepared and
mounted accordingly and autoclaved together with the culture ves-
sel.
8.1.1 Checking Gaskets (O-Rings)
O-rings are used to seal all openings on the vessel and top plate.
The top plate and all internal fittings or connections are thus
equipped with O-rings. Before every use, the O-rings must be
checked that they are present, undamaged and correctly seated.
Damaged O-rings must be replaced.
INFORMATION
Wet the O-rings with 70% alcohol or a little water to facilitate re-
moving and replacing O-rings or component parts with O-rings.
Do not use silicone grease; this can affect sterilisation results.
Carry out this check as follows:
Summary of Contents for Labfors 5
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