Labfors 5 – Version for Microorganisms
Before Fermentation/Cultivation
Page
116
of
205
22 January 2019
Procedure
1.
Remove protective caps from the inoculation needle.
2.
Keep the septum collar ready for use.
3.
Connect the inoculation needle via silicone hose with an ap-
propriate container for the inoculum.
4.
Put the inoculation needle in a sterile cover or wrap it up in
some aluminium foil.
8.1.12 Preparing the Dip Tube/Addition Port Adapter for Inoculation
If later inoculation is to be carried out by means of a dip tube or
addition port adapter, proceed as follows:
Procedure
1.
Fit the dip tube with the clamping adapter or addition port
adapter in the port.
2.
Place a piece of silicon hose onto the dip tube/addition port
adapter.
3.
Equip the hose for a sterile hose connection. (Depending on
the application: rapid coupling, sterile connector or weldable
hose with sterile filter).
4.
Secure the hose transition points with cable ties.
8.1.13 Equipping Port with Septum Collar and Septum for Inoculation
A port in the vessel top plate must be appropriately prepared for
later inoculation. For this, it must be equipped with septum collar
and septum.
Summary of Contents for Labfors 5
Page 208: ......
