41
Fig. 17.
Loading the applicator
8.
Slide the loaded sample applicator into the appropriate slot on
the sample applicator arm in the separation and control unit.
Do not press down on the applicator arm or samples may
touch the gel surface.
In IEF techniques, a pre-focusing step is usually run before the sample
is applied. This pre-focusing time is programmed in the method as the
volthours elapsed before the sample applicator is lowered onto the
gel. Start the method and load the sample applicator(s) during the
prefocusing time. Loaded sample applicators can be put into the
applicator arm any time before the applicator arm goes down.
The general procedure for running PhastGel IEF media is described
below. (See Separation technique file No. 100, for running conditions
and more specific information.)
Preparing the gel compartment
1.
Switch the system on and set the standby temperature to the
temperature of the first step in the method you plan to run.
(For details see Using the keyboard, section 5.1.)
2.
Lower the electrode assembly and sample applicator arm onto
the separation bed. Then press down both red eccentric levers
until they click into place. The electrode assembly is now in its
lower position, aligned evenly with the surface of the separation
bed.
3.
Raise the electrode assembly to the vertical position.
4.
Fit the PhastGel IEF gel cover into place on the underside of the
electrode assembly. The electrodes will protrude through the
slots. Make sure the cover is aligned correctly by pressing firmly
along the sides with your thumb. Avoid touching the electrodes
with your fingers; skin proteins may distort results.
5.3 Running IEF
media
5. Operation
