5
Probable Cause
SDS buffer strips were used instead
of native buffer strips.
The native buffer strips were used in-
stead of the SDS buffer strips.
1. Strips had uneven contact with
the gel.
2. The gel was not positioned prop-
erly, so that proteins migrate too
close to one edge.
Solution(s)
Be sure to use the correct buffer
strips.
Be sure to use the correct buffer
strips.
1. Gently press down along the
buffer strips to ensure good con-
tact with the gel. Wear gloves or
use a smooth object to do this.
2. Be sure the gel is positioned
within the vertical red lines in the
separation bed.
Symptoms
Extra bands
with native-
PAGE
Long streaks without
any bands for SDS-
PAGE
Curved bands on one
or both sides of the
gel
PhastGel gradient and homogeneous media
Trouble Shooting Guide
