45
the running step is displayed. Press ”SEP real condition” to display AVh
again.
Sample application
1.
Load the applicator with sample as described earlier (p. XX).
2.
Press ”SEP pause/continue”. The SEP ON LED will blink and an
alarm will sound at 20 second intervals to remind you that the
method is paused.
3.
Open the lid and slide the applicator into the appropriate slot
(cathode, middle or anode) in the sample applicator arm.
Repeat this for the second gel.
4.
Close the lid and press ”SEP pause/continue”. The SEP ON
LED will again show a steady light.
During the course of the run you may at any time start a development
run for a finished gel or program another separation or development
method. To go back and check on the separation run in progress,
press ”SEP real condition”.
Fig. 22.
Inserting the applicators.
Stopping the run
At the end of the method an alarm will sound for 15 seconds, and will
continue to sound at one minute intervals until the method is stopped.
The method will continue to run under the same conditions as the last
step (before an empty step) in the method until the method is stopped.
1.
Stop the method by pressing ”SEP start/stop” :
PRESS
<do> TO END SEPARATION METHOD
2.
Press ”do” to confirm. The display will show the temperature of
the separation bed and the accumulated volthours for the method
just ended:
SEP 0V 0.0mA 0.0W 15°C 500 A Vh
3.
Proceed with development immediately after the method is
stopped.
5. Operation
