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8. Ordering information and technical data
8.2.4 PhastGel separation media and accessories
A list of the technical data for PhastGel separation media and accesso-
ries is given below.
Common data
Gel material
polyacrylamide
Gel backing
polyester (0.175 mm thick)
Storage
4-8°C (Do not freeze)
PhastGel IEF media
Dimensions
43 x 50 x 0.35 mm
Gel matrix
5% T; 3% C
Separation length
37 mm
Pharmalyte
®
concentration
approx. 22 µmol/ml gel • pH unit
pH gradient
linear
PhastGel gradient media
Dimensions
43 x 50 x 0.45 mm
Stacking zone composition
6% T, 3% C
Length
13 mm
Gradient zone composition
PhastGel gradient 10-15: 10-15% T; 2% crosslinking
PhastGel gradient 8-25: 8-25% T; 2% crosslinking
PhastGel gradient 4-15: 4-15% T; 1 to 2% gradient
crosslinker
Length
32 mm
Buffer system
0.112 M acetate; 0.112 M Tris in both zones.
pH
6.4
PhastGel homogeneous media
Dimensions
43 x 50 x 0.45 mm
Stacking zone
13 mm
Separation zone
32 mm
Buffer system
0.112 M acetate and 0.112 Tris in both zones.
pH
6.4
PhastGel homogeneous 20
Stacking zone
7.5% T; 3% C
Separation zone
20% T; 2% C
PhastGel homogeneous 12.5
Stacking zone
6.0 t; 3% C
Separation zone
12.5% T; 2%C
