14 Wash the system by running mobile phase at e.g. 150 ml/min for 3 minutes with the
column in Bypass mode.
•
In the System Control module, select Menu:Manual.
•
In the dialog, select Flowpath:InletA and select a wash inlet
•
Select Flowpath:Outlet and select an outlet.
•
Select Pump:Flow and enter the flow rate 150 ml/min.
•
Stop the pump after 3 minutes.
The instruction is for ÄKTApilot. The commands differ slightly for ÄKTAex-
plorer.
Note:
15 Run the column upflow for a few minutes to remove residual air possibly trapped in
the adapter. The column is now packed. Before using it, evaluation of the packing is
recommended (see Section 5.4 Performance evaluation of the column, on page 100).
16 It is recommended to replace the tubing with a stop plug [101] at the check valve
[116].
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
97
5 Operation
5.3 Manual packing of the column
