Intelligent Packing principle
Intelligent Packing is a system solution using UNICORN-controlled packing of AxiChrom
columns with an ÄKTA system. A wizard is used to select values for essential variables.
UNICORN controls the flow of hydraulic liquid into the hydraulic chamber, which pushes
the adapter down from its start position (A) (Figure 3.3). The user watches for the exact
point when the adapter comes into contact with the consolidated bed surface (B) and
clicks on a button in a UNICORN dialog to signal the start of compression. The adapter
continues to move downwards and thereby compresses the bed according to the pre-
determined Packing Factor to obtain the target bed height (C). During compression there
is a rise in pressure (X). When compression is complete UNICORN stops the flow of hy-
draulic liquid, for some media flow conditioning will then start. If selected in the Intelligent
Packing wizard in UNICORN, an evaluation test of the packed bed is automatically per-
formed.
A
B
X
C
Adapter height
Column pressure
Figure 3.3: Principle of Intelligent Packing. The adapter starts from a set position (A) and
initiates the consolidation of the bed. On contact with the consolidated bed surface (B) the
user indicates the start of the bed compression. Compression occurs according to a pre-
determined Packing Factor. Compression of the bed causes an increase in the pressure
(X). Following compression the target Packing Factor is attained (C).
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
21
3 Specifications and characteristics
3.1 Principles of column function
