Inlet and outlet functions
•
Top mobile phase inlet/outlet on the adapter - note that the column may be run
upflow or downflow.
•
Hydraulic chamber vent valve on the top plate is used to get rid of air trapped in the
hydraulic chamber. Viewed from above, this valve has a small hole and is fitted with
a round nipple.
•
Hydraulic chamber outlet on the top plate is used to channel the hydraulic liquid out
of the column during unpacking. Viewed from above, this outlet has a larger hole
than the hydraulic chamber vent valve, and is fitted with a hexagonal stop plug.
•
Hydraulic chamber inlet on the top bayonet is used to fill the hydraulic chamber. The
hydraulic chamber inlet has an integrated check valve.
•
Bottom mobile phase inlet/outlet on the bottom plate - note that the column may
be run upflow or downflow.
Port inner diameter (mm) of inlets and outlets
Column diameter (mm)
Inlet/outlet connection thread
100
70
50
2.9 mm
2.9 mm
1.7 mm
Bottom mobile phase inlet/outlet, 5/16”
2.9 mm
2.9 mm
1.7 mm
Top mobile phase inlet/ outlet, 5/16”
2.9 mm
2.9 mm
1.7 mm
Internal adapter tubing, 5/16”
2.9 mm
1.7, 2.9
1
mm
1.7 mm
Adapter
2.0 mm
1.5 mm
1.5 mm
Hydraulic chamber inlet, 5/16”
4.0 mm
3.5 mm
3.0 mm
Hydraulic chamber outlet, 5/16”
0.9 mm
0.9 mm
0.9 mm
Hydraulic chamber vent valve, M8
1
Dependant upon media e.g.: 1.7 mm for Sepharose HP, 2.9mm for Capto media.
20
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
3 Specifications and characteristics
3.1 Principles of column function
