5.3
Manual packing of the column
CAUTION
Always use an appropriate pressure alarm or preferably a safety
valve.
CAUTION
If the hydraulic chamber vent valve is opened while there is pres-
sure in the hydraulic chamber, liquid will spray out. Take necessary
precautions to avoid personal injury or damage to equipment.
Manual packing with ÄKTA
system
AxiChrom columns can be manually packed using an ÄKTA system and this may be
useful when, for example, developing new methods that demand definition of other
packing parameter values than those allowed in the Intelligent Packing wizard. Even if
the wizard is not used, the strategy containing Intelligent Packing allows access to some
useful method instructions (see below).
CAUTION
Ensure that the pivot stand locking pin is secured before starting
the process so that the column cannot turn over by accident.
1
Prepare the media slurry (see Preparing the slurry, on page 63). Remember to com-
pensate for the compression and the Slurry Volume Factor when calculating the
slurry volume needed to achieve the target bed height. The target bed height is cal-
culated from the consolidated bed height and the Packing Factor.
Packed bed height =
consolidated bed height
packing factor
2
Transfer the medium into a suitable packing liquid, (see Preparing the slurry, on
page 63).
3
Connect the AxiChrom column to a compatible ÄKTA system (see Section 4.9 Instal-
lation in basic configuration, on page 57).
4
Prime the column (see Priming the column, on page 73).
94
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
5 Operation
5.3 Manual packing of the column
