5.1
Intelligent Packing of the column with ÄKTAexplorer
and ÄKTApilot
Intelligent Packing allows for convenient and precise control of the column packing using
AxiChrom column together with ÄKTA system, UNICORN software, and verified packing
methods. Several BioProcess™ media are available from GE Healthcare that have been
verified for use with AxiChrom column and Intelligent Packing. These are found in a list
of media in the method wizard for Intelligent Packing.
NOTICE
If using ÄKTApilot system ensure that the containers holding the
liquids are placed on a surface lower than the level of the system.
This is to ensure that there is no flow of liquid in the tubing when
the flow rate is set to zero.
NOTICE
Ensure that the air trap is filled with mobile phase liquid. This is
because the methods created using the Intelligent Packing wizard
are constructed so that the airtrap is in line with the column when
equilibration and testing operations are being performed. These
require that flow occurs into the process chamber via the mobile
phase inlet/outlet. By contrast, during packing, the method is con-
structed so that the air trap is bypassed as hydraulic liquid is
pumped into the hydraulic chamber of the column.
Preparing the slurry
Prior to filling the column, it is necessary to calculate the volume of slurry, based on
slurry concentration, needed to achieve the target bed height. The Intelligent Packing
wizard in UNICORN calculates the slurry volume needed based on parameters selected
in the wizard.
Slurry volumes presented in the wizard assume that the medium is suspended
in 20% (v/v) ethanol. Any other buffer will not give an accurate calculation of
slurry concentration according to the recipe.
Note:
The achieved bed height may differ slightly from the target bed height due to,
for example how accurately the slurry concentration was measured and the
actual volume added to the column.
Note:
The volume of slurry may not exceed the volume capacity of the column.
Note:
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
63
5 Operation
5.1 Intelligent Packing of the column with ÄKTAexplorer and ÄKTApilot
