For ÄKTAexplorer
In the UNICORN System Control module, select Manual in the menu and Flow-
path:ColumnPackingPosition. Open the correct position for valve V2 and valve V3.
The instructions in the examples above require installation of the strategy
containing Intelligent Packing, and selection of the Intelligent Packing
component.
Note:
9
Pump hydraulic liquid into the hydraulic chamber using flow velocities from Table 3.4.
10 Close the hydraulic vent valve when no air remains in the hydraulic chamber, i.e.
liquid has been forced into the syringe and no more air bubbles come, and remove
the syringe. The adapter starts moving down and the media is packed.
NOTICE
Make sure to remove the syringe otherwise it may become
hooked under the adapter rod top and hinder the packing, es-
pecially if packing a low bed height.
11 Note when the adapter comes into contact with the consolidated bed.
There are different scales for stainless steel and plastic bed supports on
the column.
Note:
12 Continue to compress the bed until the target bed height is reached. Calculate the
achieved Packing Factor (PF).
or,
Continue to compress until the target Packing Factor (PF) is reached.
PF=
consolidated bed height
packed bed height
Read the bed height. Calculate the achieved Packing Factor (PF).
13 Turn off the pump when the target bed height is reached.
96
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
5 Operation
5.3 Manual packing of the column
