Bioneer Exicycler 384, User Manual

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www.bioneer.com
BQ-042-101-12
Revision : 0 (2018-07-05
Exicycler
™
384
Reagents and Consumable Products
Various reagents and kits are required to perform a Real-Time PCR in Exicycler ™ 384 Real-Time
Quantitative Thermal Block. Please refer to this section to obtain detailed information about the appropriate
reagents and kits for your experiments. For ordering, please go to the Ordering Information.
PCR Premix kit
AccuPower
®
Greenstar
™ qPCR Master Mix kit allows an easy and fast amplification in
Exicycler
™ 384.
AccuPower
®
Greenstar ™ qPCR Master Mix kit consists of Greenstar
®
fluorescent dye, HotStart Taq DNA
polymerase, and necessary qPCR components. Therefore, only template, primers, and DW need to be
added to start your amplification.
Optical 384-well plate
You must use a plate when performing Real-Time Quantitative PCR in
Exicycler
™ 384. A standard plate can be also used, but only for a
conventional PCR without fluorescent dyes.
Optical sealing tape
Seal up the optical plates tightly with the optical sealing tape for
fluorescence detection. The sealing tape is available in the size of 384-
well plate.
You must cut up the sealing tape with a box cutter, not scissors. The tape adhesive can be carried over
to the surface of sealing tape when cutting up the tape with scissors. This will make the plate stick to
the heating lid of
Exicycler ™ 384. Plate stuck to the lid will drop inside Exicylcer ™ 384 when the lid is
cooled down.
Chemical hazard:
AccuPower ™ Greenstar™ qPCR Mater Mix may cause eye and skin irritation, and respiratory tract
irritation. Read MSDS before use and follow the instructions if swallowed or inhaled. Wear appropriate
protective eyewear, clothing, and gloves.