Bioneer Exicycler 384 User Manual Download Page 2

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Exicycler

™

384

Exicycler

™ 384

Real-Time Quantitative Thermal Block

User’s Guide

Bioneer Corporation
8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon
34302, Republic of Korea

Tel: +82-42-930-8777
Fax: +82-42-930-8688
Email: [email protected]
www.bioneer.com

Summary of Contents for Exicycler 384

Page 1: ...Exicycler 384 Real Time Quantitative Thermal Block 35 15 MT Promedt Consulting GmbH Altenhofstr 80 D 66386 St Ingbert Germany Tel 49 6894 58 10 20 A 2061...

Page 2: ...er 384 Real Time Quantitative Thermal Block User s Guide Bioneer Corporation 8 11 Munpyeongseo ro Daedeok gu Daejeon 34302 Republic of Korea Tel 82 42 930 8777 Fax 82 42 930 8688 Email sales bioneer c...

Page 3: ...y of the purchaser to determine the suitability of the product for its particular use Liability is conditional upon the customer providing full details of the problem to Bioneer within 30 days Tradema...

Page 4: ...Exicycler 384...

Page 5: ...m Views 15 Installation and System Operation 16 Overview 17 Site Requirements 17 Required Materials 17 Unpacking Exicycler 384 17 Setting Up the Computer 18 Connecting the Computer and Exicycler 384 1...

Page 6: ...1 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Replacing Fuses 63 Returning Exicycler 384 for Service 64 Troubleshooting 65 Ordering Information and Warranty 70...

Page 7: ...2 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Before Starting...

Page 8: ...384 please visit us online at www bioneer com How to Reach Customer Support To obtain prompt customer support please call us at 82 42 930 8777 You can also obtain technical support through www bioneer...

Page 9: ...temp ratures pendant plusieurs heures Risque de condensation pouvant entrainer des fonctionnements d fectueux de l quipement English Be aware of the door Please pay attention to the door when it is mo...

Page 10: ...t may be displayed on Exicycler 384 Indicates On position of the main power switch Indicates Off position of the main power switch Indicates a standby switch by which the instrument is switched on to...

Page 11: ...6 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Safety Instructions...

Page 12: ...icycler 384 and the wall for proper ventilation 5 Do not install Exicycler 384 in a dusty environment to prevent false operation or technical damage 6 Keep Exicycler 384 away from heat sources 7 Exicy...

Page 13: ...turn off the computer and unplug the power cable before connecting it to Exicycler 384 If the computer is turned on while connecting the communication port connector either in the computer or Exicycl...

Page 14: ...Time PCR run is done Wait until the cooling fan has stopped running completely Cooling fan remains running for about 2 minutes to cool down the lamp after each Real Time PCR run 6 Do not cover Exicyc...

Page 15: ...10 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Introduction...

Page 16: ...ing technique based on polarization of light enhances detection sensitivity for robust and reliable results Patented KR10 1089045 US8427643 polarizing optical apparatus mitigates the common problem of...

Page 17: ...12 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 System Components and Specifications...

Page 18: ...ystem component listed below System Components Component Lists Check Exicycler 384 1 USB 2 0 high speed cable 1 Power cord 1 1 Software 1 CD User Guide 1 Optical tape sealing applicator 1 1 Provided a...

Page 19: ...urce Short arc lamp 120W Sensor 16 bit 2D CCD Excitation Filter Emission Filter 5 Set Continuously turning on and off the lamp reduces the lamp life span The lamp generates heat when it starts Make su...

Page 20: ...r com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 irritation Read the MSDS before use and follow the instructions if swallowed or inhaled Wear appropriate protective eyewear clothing and gloves...

Page 21: ...16 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Installation and System Operation...

Page 22: ...om water Required Materials Scissors pocket knife or box cutter Unpacking Exicycler 384 Save the packing materials and box in case you need to ship the instrument to Bioneer for service 1 To unpack Ex...

Page 23: ...SB data transfer 2 It is recommended to install an anti virus software in order for Exicycler 384 to operate safely A firewall should also be setup to prevent unwanted information coming in from exter...

Page 24: ...ver To install Bioneer Package and Exicycler384 softwares for an Exicycler 384 device under Windows 7 follow the instructions below If a previous version of software has been installed on your compute...

Page 25: ...all program features Custom installation is recommended only for advanced users Click Next 5 InstallWizard is ready for installation If you want to review or modify your settings click Back Click Inst...

Page 26: ...allation will begin 9 R program will be installed automatically Please wait until installation is complete 10 Click OK for installing R D COM interface 11 R icon will be shown on the Desktop when inst...

Page 27: ...rk 1 1 Package run dotnetfx_eng exe file of the C Bioneer_Package Net11 folder 2 Click Yes for installing NET Framework 1 1 Package 3 A setup window asking about the license agreement is displayed 4 S...

Page 28: ...23 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 6 Installation of NET Framework 1 1 is complete Click OK to finish installation...

Page 29: ...provided CD you can see setup exe file and double click InstallSheild Wizard to install required items automatically 2 Click Next and go to the next step 3 Click Install to begin installation If you...

Page 30: ...top these icons are created when installation is completed successfully 7 In the C Exicycler384 Run folder folders and files that are shown on the image below are created Copy Calibration folder from...

Page 31: ...ld connect Exicycler 384 system to a computer using provided USB cable 12 Turn on Exicycler 384 The status LED will be blue when the power is supplied properly 13 Press the power button for a second t...

Page 32: ...27 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 16 Now you are ready to operate Exicycler 384 system...

Page 33: ...this path following message will be displayed 2 Click OK to change the path 3 You should assign the path of the Calibration folder to be copied automatically Click Open and you will see the following...

Page 34: ...hat the device is not turned on or the appropriate device driver has not yet been installed on the computer If you see this message despite the instrument is turned on then you should re install the d...

Page 35: ...installation was performed previously Bioneer Package may not be installed correctly Go to Control Panel Programs Add Remove and remove 4 programs from the computer Exicycler 384 V1 1 0 Bioneer packa...

Page 36: ...sis is complete Exicycler 384 is now ready to operate The status LED will turn red when the self diagnosis fails or excessive physical shock is applied from outside e g Make sure that there are no obj...

Page 37: ...uble click Run Exicycler384 icon on the desktop again IMPORTANT The following error message will appear when the calibration has not been performed for Exicycler 384 or the calibration data is not pre...

Page 38: ...options in Probe List or add your own Ensure you specify an appropriate probe option for accurate data analysis 5 Probe Information window will appear Select one or click Add to add additional probe A...

Page 39: ...ave or open a file b User Select or create the user name for personal account management c Protocol Information Displays a cycling protocol in detail d Temperature Profile Displays a temperate curve o...

Page 40: ...ific amplification products when SYBR Green is used for Real Time PCR e Gradient is used to evaluate an optimum annealing temperature condition for amplification f Store keeps the 384 well thermal blo...

Page 41: ...cycling is complete The heated lid will start cooling down automatically Exicycler 384 will keep the main door closed and will maintain thermal block at the set temperature until you stop Exicycler 38...

Page 42: ...the To field i e 94 a temperature interval in the Between field i e 1 and a hold time in the Hold Time field i e 1 16 Click Append 17 Click Incubate to add finish step i e 25 C 1 min and then click A...

Page 43: ...temperature in the From field ending temperature in the To field Press Gradient Calculator button below the time setting window and Gradient Calculator window will appear It will show predicted temper...

Page 44: ...information for each sample c Probe Name Flu Dye Quencher Type Concentration specifies a name a fluorescence dye a quencher dye a type and a concentration for each sample d Protocol Plate tab switches...

Page 45: ...Select appropriate probes and then click OK a Probe Name displays probe name assigned by the user b Fluorescent Dye displays name of a fluorescence dye assigned by the user c Quencher displays name of...

Page 46: ...Type drop down list Select the check box of Probe Name and then click Assign a Sample represents unknown samples b STD stands for a standard sample of a known concentration c NTC stands for No Templa...

Page 47: ...rough E8 and enter a sample name in the Sample Name field Click Sample Name to save sample name 28 Verify information on each well in the Name Count Probe window 29 Enter a plate name in the Plate fie...

Page 48: ...saved master file by clicking button from Quick Start menu The master file includes both protocol and plate file Power off Standby 32 Ensure the status LED in front of the Exicycler 384 is blinking gr...

Page 49: ...ing three message boxes will appear in the following order a This message will appear when closing the door of the Exicycler 384 b This message will appear when the lamp condition is being checked c T...

Page 50: ...f the pop up window remains more than 5 minutes after running Exicycler 384 or if the temperature profile does not display anything in the main window turn off Exicycler 384 and turn it on again Start...

Page 51: ...l 384 well plate You must use a plate when performing Real Time Quantitative PCR in Exicycler 384 A standard plate can be also used but only for a conventional PCR without fluorescent dyes Optical sea...

Page 52: ...47 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Performance Maintenance...

Page 53: ...POWER button 2 Go to C Exicycler384 Run and double click ExiCfg exe to start the program 3 Go to Tools Self Test NOTE USB Communication is NOT initialized message box will pop up if Exicycler 384 is t...

Page 54: ...ll of the self diagnosis tests pass completely without any problems NOTE Self diagnosis is complete with two short beeps 6 When any of the self diagnosis tests fail NG signs will appear in red NOTE An...

Page 55: ...he Fluorescence test kit is available for reuse up to 3 times For more information about the Fluorescence test kit please see the following Before Starting Calibration with the Fluorescence test kit C...

Page 56: ...e test plate in a rack and centrifuge for 5 minutes NOTE It is important to centrifuge the Fluorescence test kit prior to use This will spin down remains of fluorescence dye on the side of the well an...

Page 57: ...nging a light source lamp A periodical calibration of every 6 months helps to maintain the optimum condition of Exicycler 384 Use the ExiCfg program to calibrate Exicycler 384 The ExiCfg calibrates as...

Page 58: ...84 and turn it on again after 5 seconds In case of an abnormal shutdown like this wait about 5 minutes then click Lamp warming to turn on the lamp again It takes approximately 10 minutes to reach the...

Page 59: ...again to close the door 7 Click Mask Calibration Check button 8 Click Start button in the Mask Calibration window NOTE The Calibration time is approximately 10 minutes depending on the condition of Ex...

Page 60: ...e plate back into its packaging and return it to the freezer High temperature Wait at least 5 seconds before taking out the Fluorescence test plate from the 384 well block The Fluorescence test plate...

Page 61: ...k Calibration 2 Place the Background Fluorescence test plate on the block IMPORTANT It is important to load the Fluorescence test plate in the right position A misplaced plate may cause damage or tech...

Page 62: ...ccessfully complete Put the plate back into its packaging and return it to the freezer High temperature Wait at least 5 seconds before taking out the Fluorescence test plate from the 384 well block Th...

Page 63: ...e when performing the Background Calibration followed by the Mask Calibration 2 Click Multi Channel Calibration button 3 Select fluorescence dyes in the Multi Channel Calibration window for the Calibr...

Page 64: ...e plate named X i e FAM on the bath message appears 6 Place the Multi Channel Fluorescence Test plate on the block IMPORTANT It is important to load the Fluorescence Test plate in the right position A...

Page 65: ...e test plate from the 384 well block The Fluorescence test plate is heated to a temperature of 100 C and may cause a burn 9 Prepare the Fluorescence Test plate with a dye X i e SYBR Green when Attach...

Page 66: ...t from the top menu bar to close ExiCfg program IMPORTANT Wait at least 5 minutes to switch off main power of Exicycler 384 The cooling fan will continue to run to cool down the lamp Shutting down Exi...

Page 67: ...h block and set it aside 3 Use a cotton swab soaked in pure isopropyl alcohol or DNA Zap to clean the wells thoroughly Make certain that the isopropyl alcohol or DNA Zap has evaporated completely befo...

Page 68: ...igh voltage power supply To avoid electrical shock disconnect the power supply to the instrument unplug the power cord and wait at least 1 minute before working on the instrument 2 Insert a small scre...

Page 69: ...to Bioneer for service 2 Complete certification for instrument decontamination 3 Fax or e mail exicycler support bioneer com the service request form to the customer center 4 Pack the instrument in th...

Page 70: ...65 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Troubleshooting...

Page 71: ...f the same error keeps occurring No communication detected between the computer and Exicycler 384 1 Make sure that the USB cable is firmly connected to the computer 2 Ensure you use the USB cable prov...

Page 72: ...ng lid works or if the sample tubes or plates are completely sealed with the sealing tape 2 Ensure the heating lid option is set up correctly The Hottop Check Box must be selected and correct temperat...

Page 73: ...ating with each other Check if the USB cable between the computer and Exicycler 384 is firmly connected 13 You must insert New Dye Name will appear if a new name is not assigned to a custom dye 14 You...

Page 74: ...ta ID do not match 14 Please insert any protocol before inserting Goto Protocol will appear if the Goto step is inserted in the first line of a protocol file 15 Please insert Data name will appear if...

Page 75: ...70 www bioneer com BQ 042 101 12 Revision 0 2018 07 05 Exicycler 384 Ordering Information and Warranty...

Page 76: ...Real Time Quantitative Thermal Block Plastic consumables 3111 41 Adhesive Optical Sealing Film 100 sheets Premix Reagent K 6253 AccuPower 2X Greenstar qPCR Master Mix 100Rxn 50 L reaction K 6254 AccuP...

Page 77: ...for five years from the initial instrument release date If a defect arises after the limited warranty period shipping and handling charges may apply to any repairs or exchanges of the product underta...

Page 78: ...bioneer com www bioneer com 155FilbertSt Suite216Oakland CA94607 USA 1 877 264 4300 Toll free 1 510 865 0350 order usa bioneer com us bioneer com KoreaBioParkBLDG B 702 700Daewangpangyo ro Bundang gu...

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