Bioneer Exicycler 384, User Manual

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www.bioneer.com
BQ-042-101-12
Revision : 0 (2018-07-05
Exicycler
™
384
NOTE:
Use the left mouse button when selecting a single well. When selecting a range of cells, click the first
cell in the range, and then drag to the last cell. You can also select cells in a row or column by
pressing CTRL and clicking the row or column heading. To select all cells in 384-well plate, click the
cell at the top left corner of the diagram.
24) Click the probe option cell and then select STD in the Type drop-down list. Select the check box of
Probe Name and then click Assign.
a. Sample represents unknown samples.
b. STD stands for a standard sample of a known concentration.
c. NTC stands for No Template Control and is a sample without a template.
d. IPC stands for Internal Positive Control and is a sample that monitors the PCR run during
Existence / Non-existence reaction. It will also diagnose cause of the negative result from
PCR.
e. IPC- is a sample that does not contain a template of IPC.
25) For example, Click well D3 and enter ‘1000000’ for a concentration of 10
6
-copy and then click
Assign. Repeat this for wells D4 through D8 with concentrations of serial diluted standards.