ZEISS
OPERATION
Axio Imager 2
Illumination and contrast methods
168
430000-7544-001
01/2016
•
If there is not perfect congruence (Fig. 196/
A
),
use the two SW 1.5 screwdrivers (Fig. 195/
1
and
4
) to turn the two centering screws
2
and
3
) to recenter the bright phase
stop until complete congruence with the dark
phase ring is achieved (Fig. 196/
B
).
•
Finally, remove the auxiliary microscope from
the tube and reinsert the eyepiece.
Normally, however, no centering is required, since
the phase stops are factory-centered and the
centering is retained even if the universal
condenser is removed from the condenser carrier
and re-attached.
To enhance the image contrast, an interference
wide-band filter, green 32 x 4, can be inserted in
the color glass holder (requires color glass holder
for 32 mm filter size).
Perfect phase contrast is only achieved if the bright
phase stop (in the condenser) and the dark phase
ring (in the objective) are exactly congruent in the
illumination beam path (Fig. 196/
B
).
Fig. 195
Centering the phase stop on the
universal condenser
Fig. 196
Centering the phase stop (bright
ring in condenser) to the phase ring
(dark ring in objective)
