Axio Imager 2
OPERATION
ZEISS
Illumination and contrast methods
01/2016
430000-7544-001
193
•
Switch on the HBO 100 mercury vapor short-arc
lamp (Fig. 211/
1
) and allow it to warm up to
operating temperature for about 15 minutes.
•
On the reflector turret (Fig. 211/
2
), select the
reflector module FL P&C containing the desired
fluorescence filter combination (depending on
the desired kind of excitation) and swivel it in.
•
Remove the reflected-light shutter RL from the
light path in the reflected-light illuminator and
close the transmitted-light shutter TL.
•
Remove one eyepiece from the tube and set the
aperture diaphragm with bare eye. To do so,
open the aperture diaphragm (Fig. 211/
4
) until
it clears the full exit pupil of the objective.
Center the aperture diaphragm to the exit pupil
using the two centering screws (Fig. 211/
3
and
5
), if necessary.
•
Reinsert the eyepiece in the tube and close the
luminous-field diaphragm (Fig. 211/
7
) until it is
visible in the field of view.
•
Use the two centering screws (Fig. 211/
6
and
8
)
to center the luminous-field diaphragm to the
edge of the field of view.
•
Open the luminous-field diaphragm until it just
disappears from the field of view or, if there is a
risk of specimen bleaching, close it until it is
visible in the field of view.
•
Finally, refocus on the specimen and optimize
the position of the HBO 100 collector as
described in Section 3.33.3. Adjust the collector
so that the field of view is illuminated as evenly
as possible when using the short-wave
excitation reflector module. When long-wave
excitation modules are used, no correction of
the collector position is required.
Fig. 211
Epi-fluorescence components on
Axio Imager 2
