ZEISS
FIRST-TIME SET-UP
Axio Imager 2
Rotary stage Pol
76
430000-7544-001
01/2016
3.36.4
Centering objectives
The 6-position objective nosepiece Pol of the
Axio Imager is equipped with five centering
objective mounts (without DIC slot) and one
objective mount with DIC slot (non-centering).
This mount is centered relative to the stage.
Accordingly, all objectives can be centered relative
to the rotary stage.
Stage centering is necessary to ensure that a
specimen detail located in the center of the field of
view does not shift when the stage is rotated. By
centering all objectives, the specimen detail
remains in the center of the field of view even after
the objective has been changed.
•
Before centering the stage, the microscope
illumination must first be adjusted using
KÖHLER's rules (see Section 4.12.1).
•
To center the stage, use a high-contrast
specimen and an eyepiece with crossline reticle.
•
First turn the nosepiece to swing the non-
centerable objective mount (Fig. 69/
1
) (mount
with DIC slot) into the light path. Center the
rotary stage for the non-centerable objective
mount as described in 3.36.3.
•
Turn the nosepiece to move a centerable
objective mount into the light path (Fig. 69/
2
).
•
Rotate the stage to determine the maximum specimen deflection (Fig. 69/
3
, tip of arrow) to the
eyepiece reticles.
•
Turn each of the two centering screws on the nosepiece (Fig. 69/
4
) using an SW 1.5 Allen key
5
) to move the specimen detail by half of the arrow length towards the reticle center. Check
whether the specimen detail moves when the stage is rotated again; repeat the procedure, if required.
•
Center the other five objectives in the same manner.
To preserve this centered state it is advisable to change the objectives only by turning the
nosepiece using the knurled ring.
Fig. 69
Centering rotary stage and
objectives
