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3-1

Changing the Mode of Operation

This chapter describes how to prepare the mass spectrometer for the

following modes of operation:
•

ESI (electrospray ionization)

•

ESCi (combined electrospray and atmospheric pressure chemical

ionization)

•

APCI (atmospheric pressure chemical ionization)

•

Combined Atmospheric Pressure Photoionization (APPI/APCI)

•

NanoFlow

For details about other Waters and third-party source options, refer to

the documentation supplied with the source.

Contents:

Topic

Page

Combined APPI/APCI source

3-9

NanoFlow source

3-16

Содержание Xevo TQD

Страница 2: ...ng from its use Trademarks ACQUITY UPLC ACQUITY UPLC Connections INSIGHT ESCi Waters and Xevo are registered trademarks of Waters Corporation IntelliStart IonSABRE MassLynx T Wave THE SCIENCE OF WHAT...

Страница 3: ...ontacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide Visit www waters com Telephone and fax From the USA or Canada phone 800 252 HPLC o...

Страница 4: ...fall below 400 kPa 4 bar 58 psi during analyses that require flammable solvents Connect to the LC output with a gas fail connector to stop the LC solvent if the nitrogen supply fails Warning To avoid...

Страница 5: ...rsonal injury ensure equipment placed on top of the Xevo TQD does not exceed 15kg Warning To avoid injuries from broken glass falling objects or exposure to toxic or biohazardous substances never plac...

Страница 6: ...e enclosure assembly can become hot To avoid burn injuries ensure the source heater is turned off and the ion block is cool before performing maintenance on these components Warning To avoid personal...

Страница 7: ...the extent of decontamination required and the level of residual contamination permissible That authority must also prescribe the method of decontamination to be used and the appropriate protection f...

Страница 8: ...Definition Manufacturer Date of manufacture Part number Serial number Supply ratings Authorized representative of the European Community Confirms that a manufactured product complies with all applica...

Страница 9: ...acceptable calibration methods using at least five standards to generate a standard curve The concentration range for standards must cover the entire range of quality control samples typical specimen...

Страница 10: ...recipitation liquid liquid extraction LLE or solid phase extraction SPE to remove matrix interferences Whenever possible verify method accuracy and precision using matrix matched calibrators and QC sa...

Страница 11: ...nductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class A products are suitable for use in commercial that is nonresidential locations and can b...

Страница 12: ...xii...

Страница 13: ...classification xi ISM Classification ISM Group 1 Class A 1 xi EC authorized representative xi 1 Specifications and Operating Modes 1 1 Uses and compatibility 1 2 ACQUITY H Class and nanoACQUITY Xevo T...

Страница 14: ...17 Constant neutral loss mode 1 19 Sample inlet 1 19 Leak sensors 1 20 Vacuum system 1 20 Rear panel connections 1 21 2 Preparing for Operation 2 1 Starting the mass spectrometer 2 2 Verifying the ins...

Страница 15: ...e 3 13 Removing the IonSABRE II probe and APPI APCI source enclosure 3 15 NanoFlow source 3 16 Installing the NanoFlow source 3 17 Fitting a borosilicate glass capillary nanovial 3 19 Positioning the...

Страница 16: ...urce components 4 27 Cleaning the sampling cone assembly 4 28 Removing the sampling cone assembly from the source 4 28 Disassembling the sampling cone assembly 4 30 Cleaning the sample cone and cone g...

Страница 17: ...be tip and gasket 4 67 Replacing the ESI probe sample capillary 4 69 Removing the existing capillary 4 69 Installing the new capillary 4 73 Cleaning the IonSABRE II probe tip 4 77 Replacing the IonSAB...

Страница 18: ...115 Replacing the instrument s fuses 4 117 A Safety Advisories A 1 Warning symbols A 2 Task specific hazard warnings A 2 Specific warnings A 3 Caution symbol A 5 Warnings that apply to all Waters inst...

Страница 19: ...hernet cables B 19 I O signal connectors B 20 Signal connections B 22 Connecting to the power supply B 25 C Materials of Construction and Compliant Solvents C 1 Preventing contamination C 2 Items expo...

Страница 20: ...xx Table of Contents...

Страница 21: ...onnections for gas and plumbing Contents Topic Page Uses and compatibility 1 2 Ionization techniques and source probes 1 9 IntelliStart Fluidics system 1 11 Ion optics 1 13 MS operating modes 1 14 MS...

Страница 22: ...ion combined electrospray ionization and atmospheric pressure chemical ionization ESI APCI ESCi source Requirement Dedicated APCI operation requires an additional probe IonSABRE II Optional dual mode...

Страница 23: ...Uses and compatibility 1 3 Xevo TQD TP03406...

Страница 24: ...1 4 Specifications and Operating Modes Xevo TQD with visor up TP03407...

Страница 25: ...TY H Class and nanoACQUITY Xevo TQD UPLC MS systems The Waters Xevo TQD is compatible with the ACQUITY UPLC H Class and nanoACQUITY UPLC systems If you are not using either system refer to the documen...

Страница 26: ...rolling Contamination in UPLC MS and HPLC MS Systems part number 715001307 You can find the documents on http www waters com click Services and Support Support Xevo TQD with ACQUITY UPLC system Xevo T...

Страница 27: ...C System Operator s Guide or Controlling Contamination in LC MS Systems part number 715001307 You can find these documents on http www waters com click Services and Support Support Non ACQUITY devices...

Страница 28: ...ormation on using MassLynx software OpenLynx TM and TargetLynx TM application managers are included as standard software Instrument Console software The Instrument Console is a software application wi...

Страница 29: ...nt on the mass spectrometer In ESCi the standard ESI probe is used in conjunction with a corona pin to allow alternating acquisition of ESI and APCI ionization data facilitating high throughput and wi...

Страница 30: ...rate downward to 100 nL min If a syringe pump is used a gas tight syringe is necessary to effect correct flow rates without leakage A volume of 250 L is recommended Borosilicate glass capillaries nano...

Страница 31: ...UPLC System Guide for further details IntelliStart Fluidics system Functionality The IntelliStart Fluidics system is a solvent delivery system built into the mass spectrometer It delivers sample or s...

Страница 32: ...ry out the operations processed by the software You can set IntelliStart fluidics configuration requirements in the system console You can edit the parameters frequency and extent of the automation Se...

Страница 33: ...mass to charge ratios 4 The mass separated ions pass into the T Wave collision cell where they either undergo collision induced dissociation CID or pass to the second quadrupole Any fragment ions are...

Страница 34: ...he selected ion recording SIR mode for quantitation when you cannot find a suitable fragment ion to perform a more specific multiple reaction monitoring MRM analysis see page 1 15 In SIR and MRM modes...

Страница 35: ...ss Fragment precursor ions and pass all masses Scanning Precursor parent ion spectrum Scanning Static at product mass MRM Static at precursor mass Static at product mass PICS Product Ion Confirmation...

Страница 36: ...de Typical applications Product ion mode is typically used for the following applications Method development for MRM screening studies Identifying product ions for use in MRM transitions Optimizing CI...

Страница 37: ...R Because both MS1 and MS2 are static greater dwell time on the ions of interest is possible so the sensitivity achieved is better compared with scanning mode MS MS This mode is the most commonly used...

Страница 38: ...background for all other components This enables fast characterization of potential matrix effects providing a platform for more robust method development Typical application You typically use RADAR m...

Страница 39: ...Constant neutral loss mode Typical application You typically use constant neutral loss mode to screen mixtures for a specific class of compound that is characterized by a common fragmentation pathway...

Страница 40: ...ructions for complete details Vacuum system An external roughing pump and an internal split flow turbomolecular pump combine to create the source vacuum The turbomolecular pump evacuates the analyzer...

Страница 41: ...hows the rear panel locations of the connectors used to operate the instrument with external devices Instrument rear panel Power Source vent Source vacuum Nitrogen inlet Collision cell gas inlet Turbo...

Страница 42: ...1 22 Specifications and Operating Modes...

Страница 43: ...er describes how to start and shut down the instrument Contents Topic Page Starting the mass spectrometer 2 2 Preparing the IntelliStart Fluidics system 2 6 Rebooting the instrument 2 8 Leaving the ma...

Страница 44: ...the rear panel ensure the nitrogen supply is connected to the instrument s nitrogen inlet connection see the figure on page 1 21 Requirement The nitrogen must be dry and oil free with a purity of at l...

Страница 45: ...D the sample manager s run LED and the mass spectrometer s Operate LED remain off 6 Start MassLynx software and monitor the Instrument Console for messages and LED indications 7 Click IntelliStart in...

Страница 46: ...nt indicate its operational status Power LED The power LED to the top right hand side of the mass spectrometer s front panel indicates when the instrument is powered on or powered off Operate LED The...

Страница 47: ...ng to the following table If you are using an APCI interface IntelliStart software automatically sets the parameters according to the following table Flow rate versus temperature and gas flow Flow rat...

Страница 48: ...arately to infuse smaller volumes The low volume vials have a volume of 1 5 mL Required material Chemical resistant powder free gloves To install the reservoir bottles Caution To avoid accidental spil...

Страница 49: ...low volume vials 1 If a standard reservoir bottle is fitted remove it 2 Screw the low volume adaptor into the manifold and tighten it 3 Screw the low volume vial into the adaptor 4 For each low volum...

Страница 50: ...ument s solvent delivery system can require more than one purge cycle to minimize carryover Rebooting the instrument The reset button causes the mass spectrometer to reboot Reboot the instrument when...

Страница 51: ...maintenance When changing the source When leaving the mass spectrometer unused for a long period In these instances put the mass spectrometer in Standby mode See the online Help for details Emergency...

Страница 52: ...2 10 Preparing for Operation...

Страница 53: ...ray and atmospheric pressure chemical ionization APCI atmospheric pressure chemical ionization Combined Atmospheric Pressure Photoionization APPI APCI NanoFlow For details about other Waters and third...

Страница 54: ...r working on the source see page 4 7 2 Remove the protective sleeve if fitted from the ESI probe tip Warning The LC system connections ESI probe and source can be contaminated with biohazardous and or...

Страница 55: ...uster assembly 4 Tighten the probe locking ring to secure the probe in place Tip An automatic pressure test runs when the probe is correctly seated 5 Connect the ESI probe s cable to the high voltage...

Страница 56: ...ve the ESI probe 1 Prepare the instrument for working on the source see page 4 7 2 Disconnect the tubing from the ESI probe 3 Disconnect the ESI probe cable from the high voltage connector 4 Unscrew t...

Страница 57: ...i operation See the mass spectrometer s online Help for details on how to optimize the ESI probe for ESCi operation APCI mode APCI mode an option for the mass spectrometer produces singly charged prot...

Страница 58: ...deprotonated in the negative ion mode The sample and reagent ions then pass through the sample cone and into the mass spectrometer Installing the IonSABRE II probe Required materials Chemical resistan...

Страница 59: ...e probe adjuster assembly ensuring that the probe location dowel aligns with the probe adjuster assembly location hole 3 Tighten the probe locking ring to secure the probe in place Tip An automatic pr...

Страница 60: ...stant powder free gloves To remove the IonSABRE II probe 1 Prepare the instrument for working on the source see page 4 7 2 Remove the corona pin see page 4 12 3 Disconnect the selector valve tubing fr...

Страница 61: ...APPI lamp drive assembly is advanced into the source APPI mode The IonSABRE II probe introduces vaporized sample into the source where photons generated by an ultraviolet UV lamp mounted in the APPI...

Страница 62: ...aporized sample into the source The sample passes between the sample cone and the corona pin which typically operates with a discharge current of 5 A The corona discharge generates ions that react wit...

Страница 63: ...When the source is configured for dual operation in APCI mode current is applied to the corona pin but the repeller electrode is inactive Dual operation in APCI mode When the source is configured for...

Страница 64: ...e enclosure The UV lamp which you ignite via a control in the MassLynx Tune window provides a constant photon output You vary the intensity of incident radiation upon the sample molecules by adjusting...

Страница 65: ...nt for working on the source see page 4 7 Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing...

Страница 66: ...instrument s front panel connector 7 Connect the HT cable to the instrument s front panel connector 8 Install the IonSABRE II probe to the source and ensure that it is working correctly see page 3 6 T...

Страница 67: ...e from the instrument s front panel 5 Remove the source enclosure see page 4 8 6 Remove the corona pin see page 4 15 7 Fit the blanking plug to the pin s mounting contact Warning The source components...

Страница 68: ...three axis manipulator that slides on a pair of guide rails that allow its withdrawal from the source enclosure for maintenance and changes A light within the source provides illumination for the spr...

Страница 69: ...ning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shoc...

Страница 70: ...of the NanoFlow source and your sprayer Tip For details regarding how to fit each sprayer see the corresponding reference Waters Universal NanoFlow Sprayer Installation and Maintenance Guide part num...

Страница 71: ...ials Chemical resistant powder free gloves Needle nose pliers Borosilicate glass capillary Fused silica syringe needle or GELoader tip Fused silica cutter Caution The NanoFlow stage contains a high vo...

Страница 72: ...m the end of the sprayer assembly 6 Remove the existing capillary from the sprayer Warning To avoid lacerations puncture injuries and possible contamination with biohazardous and toxic samples do not...

Страница 73: ...ple Recommendation When using a GELoader tip break the glass capillary in half scoring it with a fused silica cutter so that the GELoader can reach the capillary s tip 9 Thread the knurled nut and app...

Страница 74: ...ing obtained a signal you must adjust the tip position to maximize it Using the three axis manipulator you can adjust the tip position up and down left and right forward and backward As a starting poi...

Страница 75: ...Should the spray stop you can restart it To do so in the Tune window set Capillary to 0 kV Then adjust the three axis manipulator so that viewed under magnification the capillary tip touches the sampl...

Страница 76: ...3 24 Changing the Mode of Operation...

Страница 77: ...efitting the source enclosure 4 8 Installing and removing the corona pin 4 12 Operating the source isolation valve 4 16 Removing O rings and seals 4 19 Cleaning the instrument case 4 20 Emptying the e...

Страница 78: ...e source assembly seals 4 92 Replacing the air filter 4 95 Replacing the roughing pump oil 4 97 Replacing the roughing pump s oil demister element 4 100 APPI APCI source changing the UV lamp bulb 4 10...

Страница 79: ...nt exhaust line Check daily empty as required See page 4 20 Gas ballast the roughing pump ESI weekly See page 4 22 Inspect and adjust the roughing pump oil level Weekly See page 4 25 Clean the source...

Страница 80: ...ESCi modes When the corona pin is corroded or black or the sensitivity decreases to unacceptable levels See page 4 84 Replace the IonSABRE II probe heater Options using the IonSABRE II probe only If t...

Страница 81: ...contaminate the roughing pump oil reduce this period which must be determined from experience See page 4 100 Clean the APPI APCI source UV lamp window When the window becomes visibly dirty or when the...

Страница 82: ...e collaboration option that controls the two way connection with the ACQUITY UPLC system by enabling the Connections INSIGHT iAssist service level Consult these sources for more information about Conn...

Страница 83: ...ts Warning To prevent injury always observe Good Laboratory Practice when handling solvents changing tubing or operating the instrument Know the physical and chemical properties of the solvents used s...

Страница 84: ...erate indicator is not illuminated 3 Wait 3 minutes to allow the desolvation gas flow to cool the probe and source 4 In the Instrument Console click API to stop the desolvation gas flow 5 Lift the vis...

Страница 85: ...nectors 4 Pull the source enclosure release located at the bottom right hand side outward and swing open the enclosure Warning The source components can be contaminated with biohazardous and or toxic...

Страница 86: ...closure and lift it vertically off the two supporting studs on the source adaptor housing 6 Store the cables neatly by plugging them into the cable storage positions on the rear of the source enclosur...

Страница 87: ...ster and options cables to the instrument s connectors Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves whil...

Страница 88: ...ug from the corona pin mounting contact Tip Store the blanking plug in a safe location Warning The LC system connections ESI probe and source can be contaminated with biohazardous and or toxic materia...

Страница 89: ...na pin mounting contact 4 Fit the corona pin to the mounting contact ensuring that the corona pin is securely mounted Warning The corona pin tip is sharp To avoid puncture wounds handle it with care T...

Страница 90: ...5 Close the source enclosure 6 Look through the source window and use the vernier probe adjuster to position the probe tip so that it is pointing approximately midway between the tips of the sample c...

Страница 91: ...lug to the corona pin mounting contact see the figure on page 4 13 5 Close the source enclosure Warning The LC system connections ESI probe and source can be contaminated with biohazardous and or toxi...

Страница 92: ...swing open the enclosure Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Wa...

Страница 93: ...Operating the source isolation valve 4 17 3 Close the source isolation valve by moving its handle counterclockwise to the vertical position Isolation valve handle in closed position...

Страница 94: ...e source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid puncture wounds take...

Страница 95: ...tool 1 to impale the O ring or seal 2 Pull the O ring or seal from its groove if necessary use tool 2 as an aid 3 Dispose of the O ring or seal in accordance with local environmental regulations Caut...

Страница 96: ...nt Emptying the exhaust trap bottle Inspect the exhaust trap bottle in the instrument s exhaust line daily and empty it before it is more than 10 full Nitrogen exhaust trap bottle Caution Do not use a...

Страница 97: ...xhaust trap bottle to the cap 6 Secure the nitrogen exhaust trap bottle in the upright position 7 Close the source enclosure Tip A leak test runs automatically If the test results in a failure ensure...

Страница 98: ...Gas ballast the roughing pump when these conditions apply With ESI operation once a week When the roughing pump oil appears cloudy When the vacuum pressure is higher than normal When condensate forms...

Страница 99: ...g pump 1 Use the flat blade screwdriver to turn the gas ballast valve on the pump a quarter turn to the open position Warning To avoid burn injuries take great care while working with the roughing pum...

Страница 100: ...a handle operated gas ballast valve To gas ballast the roughing pump 1 Move the gas ballast valve handle on the pump counterclockwise from the horizontal position to the vertical position Warning To...

Страница 101: ...l level appears in the roughing pump s oil level sight glass Check the oil level weekly you must maintain the oil level at or near the indicated maximum level when the pump is not operating Tip The oi...

Страница 102: ...y the O ring groove depth in the plug Over tightening does not improve the plug seal it only makes the plug difficult to remove later Tip After you add oil to the pump the following situations can occ...

Страница 103: ...components Clean the sample cone and cone gas nozzle when these conditions apply The sample cone and cone gas nozzle are visibly fouled You dismissed LC and sample related causes for decreased signal...

Страница 104: ...e source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shock ensur...

Страница 105: ...egrees moving the handle from the vertical to the horizontal position 3 Slide the sampling cone assembly out of the ion block assembly Caution Do not open the isolation valve if the sampling cone asse...

Страница 106: ...ction tool To disassemble the sampling cone assembly 1 Slide the collar to the end of the combined 2 5 mm Allen wrench and cone extraction tool 2 Insert the collar in the sample cone Warning The sampl...

Страница 107: ...ounter clockwise and then lift them to remove the sample cone from the cone gas nozzle 4 Remove the O ring from the sample cone Caution The sample cone is fragile Never place it on its tip always plac...

Страница 108: ...Appropriately sized glass vessels in which to completely immerse components when cleaning Use only glassware not previously cleaned with surfactants HPLC grade or better methanol HPLC grade or better...

Страница 109: ...sonic bath for 20 minutes b Remove any residual water from the extraction cone by immersing it in a glass vessel containing methanol and then place the vessel in the ultrasonic bath for 10 minutes 5 C...

Страница 110: ...tain a new one before reassembling the sampling cone assembly Assembling the sampling cone assembly Required material Clean chemical resistant powder free gloves To assemble the sampling cone assembly...

Страница 111: ...hat the source isolation valve is in the closed position see page 4 16 Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder...

Страница 112: ...e the sampling cone assembly into the ion block assembly 3 Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly 90 degrees moving the handle downward from the horizontal to...

Страница 113: ...e mass spectrometer s online Help for details 2 Close the source isolation valve see page 4 16 Warning The source components can be contaminated with biohazardous and or toxic materials Always wear ch...

Страница 114: ...llen wrench and cone extraction tool to unscrew the 4 captive screws that secure the ion block assembly 4 Remove the ion block assembly from the PEEK ion block support TP03130 Screws securing the Ion...

Страница 115: ...ined 2 5 mm Allen wrench and cone extraction tool to loosen the captive screws securing the 2 PEEK extraction cone retainer clips and then rotate the retainer clips clear of the extraction cone Warnin...

Страница 116: ...y sized glass vessel in which to completely immerse the extraction cone when cleaning Use only glassware not previously cleaned with surfactants HPLC grade or better methanol HPLC grade or better wate...

Страница 117: ...ion do as follows a Rinse the extraction cone by immersing it in a glass vessel containing water and then placing the vessel in the ultrasonic bath for 20 minutes b Dry the components by immersing the...

Страница 118: ...on cone to the ion block Required materials Chemical resistant powder free gloves Combined 2 5 mm Allen wrench and cone extraction tool To fit the extraction cone to the ion block 1 Fit the extraction...

Страница 119: ...ion block assembly securing screws sequentially and in small increments 3 Open the source isolation valve see page 4 16 4 Close the source enclosure Warning The source components can be contaminated...

Страница 120: ...mm Allen wrench and cone extraction tool O ring removal kit Needle nose pliers To disassemble the ion block assembly 1 Remove the ion block assembly from the source assembly see page 4 37 2 Ensure tha...

Страница 121: ...llen wrench and cone extraction tool to loosen the 2 ion block cover plate captive securing screws 6 Remove the ion block cover plate Caution To ensure correct operation of the ion block assembly afte...

Страница 122: ...ation valve O ring see page 4 19 9 If the isolation valve O ring shows signs of deterioration or damage dispose of it in accordance with local environmental regulations Warning The isolation valve O r...

Страница 123: ...e ion block 12 Holding the PEEK ion block gently use the needle nose pliers to gently grasp the heat shrink tubing on the heater cartridge assembly and carefully slide it and the PEEK terminal block o...

Страница 124: ...ve the cone gas O ring from the ion block 15 If the cover seal or cone gas O ring shows signs of deterioration or damage dispose of it in accordance with local environmental regulations Warning The co...

Страница 125: ...lock blanking plug and associated seal 17 If the blanking plug seal shows signs of deterioration or damage dispose of it in accordance with local environmental regulations Warning The blanking plug se...

Страница 126: ...cone retainer clips 19 Remove the extraction cone from the ion block Caution Take great care not to damage the extraction cone aperture when removing the extraction cone from the ion block The extrac...

Страница 127: ...stant powder free gloves Appropriately sized glass vessels in which to completely immerse components when cleaning Use only glassware not previously cleaned with surfactants HPLC grade or better metha...

Страница 128: ...lace the vessels in the ultrasonic bath for 10 minutes 4 Carefully remove the components from the vessels and blow dry them using inert oil free gas 5 Inspect each component for persisting contaminati...

Страница 129: ...ndle 3 Fit the extraction cone to the ion block 4 Fit the 2 PEEK extraction cone retainer clips to the ion block 5 Use the combined 2 5 mm Allen wrench and cone extraction tool to tighten the captive...

Страница 130: ...anol water applied to a lint free cloth to carefully clean the grooves 12 Fit the cover seal to the ion block ensuring that it is correctly seated Fit a new seal if the old one has been disposed of 13...

Страница 131: ...the ion guide assembly if cleaning the ion block and isolation valve fails to increase signal sensitivity Removing the ion block assembly and ion guide from the source assembly Required materials Chem...

Страница 132: ...ion block support 4 If any of the O rings show signs of deterioration or damage dispose of them in accordance with local environmental regulations Warning The O rings can be contaminated with biohaza...

Страница 133: ...merse the ion guide when cleaning Use only glassware not previously cleaned with surfactants Length of small diameter PEEK or PTFE tubing appropriately sized for suspending the ion guide in the glass...

Страница 134: ...a PEEK or PTFE tube into a hook shape 2 Insert one end of the hook into one of the holes in the ion guide s rear circuit board carrier Warning The ion guide PCB assemblies can be contaminated with bio...

Страница 135: ...it using inert oil free gas 7 Inspect the ion guide assembly for persisting contamination If contamination is present do as follows a Use the wash bottle containing methanol to rinse the ion guide as...

Страница 136: ...screws that secure the differential aperture to the 3 supporting rods 2 Remove the differential aperture from the ion guide Caution Sensitivity may be reduced if the differential aperture is aligned...

Страница 137: ...en use 45 45 10 methanol water formic acid 4 Place the vessel in the ultrasonic bath for 30 minutes 5 If you used formic acid in the cleaning solution rinse the differential aperture by immersing it i...

Страница 138: ...aperture is correctly aligned with the ion guide assembly look along the central axis of the ion guide from the ion entry side and confirm that the hole at the centre of the differential aperture is c...

Страница 139: ...the ion block support to the source Required materials Chemical resistant powder free gloves 3 mm Allen wrench New seals and O rings Warning The source components can be contaminated with biohazardou...

Страница 140: ...start fitting the O ring at the notch in the groove and then progressively work the ring into the groove in either direction from the notch 3 Fit the PEEK ion block support to the instrument s housin...

Страница 141: ...t powder free gloves 7 mm wrench 10 mm wrench To remove the ESI probe tip and gasket 1 Remove the ESI probe from the source see page 3 4 Warning The probe and source components can be contaminated wit...

Страница 142: ...Procedures 2 Use the 10 mm wrench to remove the probe tip Tip If the probe tip is difficult to remove use the 7 mm wrench in conjunction with the 10 mm wrench Probe tip 10 mm wrench Probe tip 7 mm wre...

Страница 143: ...obe tip is damaged dispose of it in accordance with local environmental regulations Fitting the ESI probe tip and gasket Required materials Chemical resistant powder free gloves 10 mm wrench New metal...

Страница 144: ...ulizer adjuster knob to adjust the capillary so that it protrudes by approximately 0 5 mm from the end of the probe 5 Fit the ESI probe to the source see page 3 2 Warning The probe and source componen...

Страница 145: ...nch Needle nose pliers To remove the existing capillary 1 Remove the probe from the source see page 3 4 2 Retrieve the combined 2 5 mm Allen wrench and cone extraction tool from its storage location o...

Страница 146: ...n wrench and cone extraction tool to remove the 3 screws retaining probe end cover 4 Remove the end cover and gasket from the probe assembly 5 Unscrew and remove the nebulizer adjuster knob End cover...

Страница 147: ...mple capillary 4 71 6 Use the 10 mm wrench to remove the probe tip Tip If the probe tip is difficult to remove use the 7 mm wrench in conjunction with the 10 mm wrench Probe tip 10 mm wrench Probe tip...

Страница 148: ...remove the knurled collar from the UNF coupling 10 Remove the knurled collar and conductive sleeve from the capillary 11 Use the 7 mm wrench to loosen the locknut Tip Use the 8 mm wrench to steady the...

Страница 149: ...new capillary Required materials Chemical resistant powder free gloves Combined 2 5 mm Allen wrench and cone extraction tool 10 mm wrench Needle nose pliers LC pump HPLC grade or better 1 1 acetonitr...

Страница 150: ...probe inlet connector and screw the connector finger tight into the PEEK union Rationale Doing so ensures a minimum dead volume when fitting the capillary 3 Fit the UNF coupling to the new capillary...

Страница 151: ...10 Tighten the knurled collar to the UNF coupling 11 Perform a leak test by attaching the free end of the PEEK tubing to an LC pump and pumping 50 50 acetonitrile water through it at 1 mL min If leak...

Страница 152: ...nd cone extraction tool to fit and tighten the 3 screws retaining the probe end cover 20 Return the combined 2 5 mm Allen wrench and cone extraction tool to its storage location on the source adaptor...

Страница 153: ...s To clean the IonSABRE II probe tip 1 On the Instrument Console system tree click Xevo TQ S Manual optimization 2 On the Manual Optimization page click to stop the liquid flow 3 Click Gas to start th...

Страница 154: ...2 5 mm Allen wrench and cone extraction tool To remove the existing capillary 1 Remove the probe from the source see page 3 8 2 Retrieve the combined 2 5 mm Allen wrench and cone extraction tool from...

Страница 155: ...one extraction tool to remove the 3 screws retaining the probe end cover 4 Remove the end cover and gasket 5 Unscrew and remove the nebulizer adjuster knob 6 Remove the PEEK union UNF coupling assembl...

Страница 156: ...EEK union from the UNF coupling 9 Remove the ferrule from the capillary 10 Remove the capillary from the UNF coupling 11 Dispose of the capillary and ferrule in accordance with local environmental reg...

Страница 157: ...ed PEEK tubing approximately 60 cm 24 inches long Requirement Cut the tubing squarely that is perpendicular to its horizontal axis 2 Insert one end of the red PEEK tubing in the probe inlet connector...

Страница 158: ...ee end of the PEEK tubing to an LC pump and pumping 50 50 acetonitrile water through it at 1 mL min If leakage occurs disassemble and remake the connection and repeat the leak test If the backpressure...

Страница 159: ...e extraction tool to fit and tighten the 3 screws retaining the probe end cover 20 Return the combined 2 5 mm Allen wrench and cone extraction tool in its storage location on the source adaptor housin...

Страница 160: ...h the lapping film and a methanol saturated tissue 3 Install the corona pin in the source see page 4 12 Warning The probe and source components can be contaminated with biohazardous and or toxic mater...

Страница 161: ...IonSABRE II probe heater Required material Chemical resistant powder free gloves To remove the IonSABRE II probe heater 1 Remove the probe from the source see page 3 8 Warning The probe and source com...

Страница 162: ...ew IonSABRE II probe heater Required materials Chemical resistant powder free gloves IonSABRE II probe heater Caution To avoid damaging the probe heater s electrical connections do not twist the heate...

Страница 163: ...nt see page 3 6 5 In the Instrument Console click API to start the desolvation gas Replacing the ion block source heater Replace the ion block source heater if it fails to heat when the instrument is...

Страница 164: ...lock source heater 1 Remove the ion block assembly from the instrument see page 4 37 2 Ensure that the isolation valve is closed Warning The ion block assembly can be contaminated with biohazardous an...

Страница 165: ...ater 4 89 3 Use the combined 2 5 mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate 4 Remove the ion block cover plate Ion block cover plate sec...

Страница 166: ...cone extraction tool to remove the 2 screws securing the heater wires to the PEEK terminal block 6 Use the needle nose pliers to carefully swing the ring terminal tags out of the terminal block Heate...

Страница 167: ...5 mm Allen wrench and cone extraction tool to fit and tighten the 2 screws securing the heater wires to the PEEK terminal block 12 Fit the ion block cover plate to the ion block assembly and then use...

Страница 168: ...oves O ring removal kit To remove the probe adjuster assembly probe and source enclosure seals 1 Remove the source enclosure from the instrument see page 4 8 2 Use the O ring removal kit to carefully...

Страница 169: ...emoval kit to carefully remove the following seals from the source enclosure Source enclosure seal Nebulizer gas seal Desolvation gas seal Probe adjuster nebulizer gas seal Probe adjuster assembly pro...

Страница 170: ...lean the grooves 2 Fit the new source enclosure seal to the source enclosure Tip Start by feeding the seal into the groove at the bottom right hand corner and then working around the seal in a counter...

Страница 171: ...to the probe adjuster assembly Probe seal Nebulizer gas seal 5 Refit the source enclosure to the instrument see page 4 11 Replacing the air filter You must replace the air filter annually Required ma...

Страница 172: ...96 Maintenance Procedures 3 Disconnect the probe cable from the high voltage connector and leave the cable in a position that does not obstruct the air filter grill TP03403 Air filter grill Probe cabl...

Страница 173: ...neath the metal lip 7 Close the air filter grill 8 Connect the probe cable to the high voltage connector 9 Close the source enclosure 10 Lower the instrument s visor Replacing the roughing pump oil Ch...

Страница 174: ...the pump before draining 2 Vent and shut down the instrument see the mass spectrometer s online Help for details 3 Allow the roughing pump to cool 4 Place the container for used oil under the pump s d...

Страница 175: ...n plug is clean and properly seated 10 Use the 8 mm Allen wrench to fit and tighten the roughing pump s oil drain plug Warning The roughing pump oil can be irritant or contaminated with biohazardous a...

Страница 176: ...ing situations can occur The oil level drops slightly during the first month of operation The oil changes color darkens over time After the pump runs for 12 to 48 hours a few drops of oil often appear...

Страница 177: ...ectrometer s online Help for details 2 Allow the roughing pump to cool 3 Use the 6 mm Allen wrench to remove the 4 bolts securing the exhaust flange to the roughing pump Warning The pump oil can be ir...

Страница 178: ...ully remove the exhaust flange and oil demister element from the roughing pump 5 Use the 10 mm wrench to remove the nut that secures the oil demister element to the exhaust flange TP02693 Oil demister...

Страница 179: ...rom the oil demister element 7 Remove the spring from the oil demister element 8 Dispose of the oil demister element in accordance with local environmental regulations Warning The oil demister element...

Страница 180: ...ter element slightly elevated to prevent the loss of the spring fit the exhaust flange to the oil demister element Warning The pump oil can be irritant or contaminated with biohazardous or toxic analy...

Страница 181: ...ge to the roughing pump 5 Use the 6 mm Allen wrench to fit the 4 bolts securing the exhaust flange to the roughing pump Caution The nut that secures the oil demister element to the exhaust flange must...

Страница 182: ...tion tool from its storage location on the source adaptor housing 4 Hook the short end of the Allen wrench through the ring on the back of the bulb extraction plug and tug to remove it Warning The sou...

Страница 183: ...ts storage location on the source adaptor housing 10 Close the source enclosure 11 Slide closed the instrument s source interface door APPI APCI source cleaning the lamp window The transmission of the...

Страница 184: ...g Tip An automatic pressure test runs each time you close the source enclosure and when the instrument starts Removing the APPI lamp drive assembly seals Required materials Chemical resistant powder f...

Страница 185: ...the lamp drive assembly and store it in a secure place see page 4 106 3 Retrieve the combined 2 5 mm Allen wrench and cone extraction tool from its storage location on the source adaptor housing 4 Us...

Страница 186: ...ol to remove the remaining 2 lamp drive cover screws which were hidden by the release handle 8 Slide the cover off the lamp drive 9 Use the 3 mm Allen wrench to remove the 4 source enclosure cover scr...

Страница 187: ...amp drive seals 4 111 11 Use the combined 2 5 mm Allen wrench and cone extraction tool to unscrew the 4 mounting flange screws Tip Take care not to drop the screws inside the lower cover TP03205 Mount...

Страница 188: ...Using the small Phillips screwdriver remove the electrode screw and repeller electrode 14 Use the combined 2 5 mm Allen wrench and cone extraction tool to remove the two insulator screws 15 Remove the...

Страница 189: ...emoval kit to carefully remove the O ring sealing the lamp bulb from inside the bulb holder see page 4 19 18 Use the O ring removal kit to carefully remove the two O rings from inside the lamp mountin...

Страница 190: ...from the APPI source enclosure side 20 Dispose of the O rings in accordance with local environmental regulations Warning The O rings can be contaminated with biohazard ous and or toxic materials Ensu...

Страница 191: ...eat the O ring in the small radius at the bottom of the groove Then use a suitable tool one with a circular cross section to roll the remainder of the O ring into the groove 2 Fit the lamp bulb sealin...

Страница 192: ...ing shaft insertion tool from the mounting shaft 10 Fit the PEEK insulator to the end of the mounting shaft 11 Use the combined 2 5 mm Allen wrench and cone extraction tool to fit and tighten the two...

Страница 193: ...sure release handle and secure it with the 2 screws Refit and reconnect the source enclosure to the machine refit the corona pin and probe Refer to page 3 13 for instructions Replacing the instrument...

Страница 194: ...4 118 Maintenance Procedures...

Страница 195: ...des also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and statements that apply t...

Страница 196: ...electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying text identifies the specific risk and explains ho...

Страница 197: ...ction Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran THF and nitric or sulfuric a...

Страница 198: ...surfaces of the mass spectrometer when the instrument is in Operate mode To avoid nonlethal electric shock make sure the instrument is in Standby mode before touching areas marked with this high volt...

Страница 199: ...When operating this device follow standard quality control procedures and the equipment guidelines in this section Warning Waters instruments can be used to analyze or process potentially hazardous s...

Страница 200: ...er Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren Avv...

Страница 201: ...e trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou n...

Страница 202: ...iamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi n El usuari...

Страница 203: ...Warnings that apply to all Waters instruments A 9 Nonmetallic Tetrahydrofuran THF Methylene chloride Dimethyl sulfoxide THF...

Страница 204: ...tueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes die eingebauten Sicherheitseinrichtungen unter Umst nden nicht ordnungsgem funktionieren Atte...

Страница 205: ...l instrument Vorsicht Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Ger ts gedruckt sind Attenzione per...

Страница 206: ...al symbols can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor t...

Страница 207: ...ng symbols A 13 Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright Keep dry...

Страница 208: ...A 14 Safety Advisories...

Страница 209: ...echnical Service for recommended cleaning flushing and packaging procedures See page 4 7 Contents Topic Page External wiring and vacuum connections B 2 Connecting the oil filled roughing pump B 3 Conn...

Страница 210: ...ear in the figure below Note Connectors and controls not identified in the following figure are for use by Waters engineers only Rear panel Power Source vent Source vacuum Nitrogen inlet Collision cel...

Страница 211: ...W25 center rings included in the installation kit NW25 clamps included in the installation kit 10 mm reducer fitting included in the installation kit 10 mm ID nylon tube included in the installation k...

Страница 212: ...when performing this procedure Caution To ensure correct operation of the roughing pump the pump must be installed within 1 degree of horizontal The area where the roughing pump is located must have...

Страница 213: ...sing 2 hose clamps connect the opposite end of the length of 1 inch vacuum hose in step 4 to the 1 inch OD straight vacuum port on the instrument s rear panel 6 Connect the opposite end of the 10 mm n...

Страница 214: ...age B 7 Caution The instrument requires two separate exhaust systems one for nitrogen the other for the roughing pump Vent them to atmosphere through separate exhaust lines Oil mist can severely damag...

Страница 215: ...ctions To make the electrical connections to the roughing pump 1 Connect the relay cable from the roughing pump d c connector to the pump connector on the instrument s rear panel 2 Connect the roughin...

Страница 216: ...uded in the installation kit NW25 center rings included in the installation kit NW25 clamps included in the installation kit NW40 center rings included in the installation kit Warning To avoid electri...

Страница 217: ...ng pump Warning The pump and its connections can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves when performing this procedure Caution To en...

Страница 218: ...end of the length of 1 inch vacuum hose in step 4 to the 1 inch OD straight vacuum port on the instrument s rear panel 6 Connect the opposite end of the 10 mm nylon tube to the 10 mm source vent port...

Страница 219: ...the electrical connections to the oil free roughing pump 1 Connect the relay cable from the roughing pump d c connector to the pump connector on the mass spectrometer s rear panel 2 Connect the rough...

Страница 220: ...o cut a 3 8 cm to 5 0 cm 1 5 inch to 2 inch length of 6 mm PTFE tubing 2 Connect this piece of tubing to one end of the nitrogen supply in line filter 3 Connect the remaining length of the 6 mm PTFE t...

Страница 221: ...ions 5 Attach the nitrogen regulator to the nitrogen supply 6 Install the 6 mm stud into the regulator outlet 7 Connect the free end of the long piece of 6 mm PTFE tubing to the 6 mm stud Source vent...

Страница 222: ...lok nut and ferrule to connect the 1 8 inch stainless steel tube to the collision cell gas inlet on the rear of the instrument see the figure on page B 13 2 Use the wrench to tighten the 1 8 inch Swag...

Страница 223: ...0 20 kPa 2 mbar 0 03 psi below atmospheric pressure negative pressure The exhaust connections can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gl...

Страница 224: ...ctions Exhaust trap bottle Warning Leaks in the source exhaust system can result in the release of biohazardous or toxic materials To confirm the integrity of the source exhaust system perform the fol...

Страница 225: ...the barbed fitting of the drain located at the bottom of the instrument Warning The waste line and connection can be contaminated with biologically hazardous or toxic materials Always wear chemical re...

Страница 226: ...of the waste solvent Positioning of drain tube Warning To prevent leakage of biologically hazardous or toxic materials ensure that the drain line does not crimp or bend A crimp or bend can impede flo...

Страница 227: ...ed Instrument LAN on the workstation rear panel To connect the instrument to the power source 1 Select the correct power cord for your location 2 Connect the female end of the power cord to the power...

Страница 228: ...cal connections to the rear panel from hazardous voltages by double or reinforced insulation Circuits of this type are classified as safety extra low voltage SELV Examples of circuits that are typical...

Страница 229: ...Analog Out Analog Out Ground Not Used Not Used Stop Flow Out Stop Flow Out Ground Switch 2 Out Switch 2 Out Connector I 1 2 3 4 5 6 7 8 9 10 Inject Start In Inject Start In Ground Event In Event In Sw...

Страница 230: ...ion Analog Out Used for analog chart output functionality The output voltage range is 0 to 1 V The resolution of the voltage output is 12 bits Stop Flow Out Used to stop the solvent flow if the nitrog...

Страница 231: ...he signal cable to the connector 3 Slide the clamp with the bend facing down into the protective shield 4 Insert the clamp and shield with the bend facing down into the connection cover and loosely ti...

Страница 232: ...nto the connection cover and position the clamp over the cable leads Tighten the clamp into place with the second self tapping screw 6 Place the second connection cover over the first cover and snap i...

Страница 233: ...tacle on the rear panel of the instrument 2 Connect the male end of the instrument power cord to the 200 to 240 V AC wall outlet prepared as described in the instrument Site Preparation Guide 3 Connec...

Страница 234: ...B 26 External Connections...

Страница 235: ...arning To confirm the integrity of the source exhaust system you must address any safety issues raised by the contents of this Appendix Contents Topic Page Preventing contamination C 2 Items exposed t...

Страница 236: ...on ingredients used to prepare mobile phases Items exposed to solvent Item Material Autotune reservoirs High density polyethylene Corona discharge pin mounting contact PEEK Gas exhaust port Aluminum G...

Страница 237: ...Methanol Acetonitrile Formic acid 0 1 Acetic acid 0 1 Trifluoroacetic acid 0 1 Ammonium acetate 10 mM Ammonium formate 10 mM These solvents are not expected to cause any problems with the materials i...

Страница 238: ...C 4 Materials of Construction and Compliant Solvents...

Страница 239: ...connect the selector valve to the source probes use the PEEK tubing and natural beige colored PEEK finger tight screws specified on page D 4 Warning The IntelliStart Fluidics components can be contam...

Страница 240: ...sor The letters etched on the front of the valve represent the components to which the associated port is connected Etched letters with a light background indicate inputs while letters with a dark etc...

Страница 241: ...n specifications Caution When plumbing the IntelliStart Fluidics system use only the tubes nuts and ferrules outlined below to ensure over pressure protection The use of parts not recommended here can...

Страница 242: ...ple reservoirs ports A and B is not user serviceable To replace the tubing contact Waters to arrange an engineer visit Replacement tubing specifications Port ID inches Color Length m R A and B 0 020 O...

Страница 243: ...in 3 14 APPI APCI corona pin 3 11 APPI APCI source components 3 12 ASAP probe 1 10 assembling sampling cone 4 34 B biohazard warning A 4 burst warning A 3 C cables network B 19 source APPI APCI 3 14 E...

Страница 244: ...ts ii D DART source 1 2 demister element replacing 4 5 4 100 DESI source 1 2 detector compliant solvents C 1 external wiring B 2 flow rates 2 5 I O signal connectors B 20 materials of construction C 1...

Страница 245: ...ion guide assembly 4 63 waste liquid 4 21 handling symbols A 13 hazards flammable solvents iv high temperature vi removing mass spectrometer from service vi solvent leakage iv high temperature hazard...

Страница 246: ...trometer calibrating 2 4 compatibility 1 2 leaving ready for operation 2 9 removing from service vi shock hazard A 4 specifications 1 2 starting up 2 2 tuning 2 4 MassLynx software 1 7 materials of co...

Страница 247: ...B 2 rebooting detector 2 8 removing combined APPI APCI source 3 15 corona pin 4 15 ESI probe 3 4 ion block assembly 4 37 IonSABRE II probe 3 8 mass spectrometer from service vi O rings 4 19 sampling...

Страница 248: ...ning 4 18 operating 4 16 preparing for work on 4 7 source components cleaning 4 27 sources APCI 1 9 APGC 1 2 APPI APCI 1 2 combined APPI APCI 1 9 DART 1 2 DESI 1 2 ESI 1 9 LDTD 1 2 NanoFlow ESI 1 2 1...

Страница 249: ...ions B 2 system 1 20 valve source isolation 4 16 W warning symbols A 2 A 5 waste line connecting B 17 liquid handling 4 21 wiring external B 2 workstation connecting B 19 X Xevo TQD removing from serv...

Страница 250: ...Index 8...

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