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3
OPERATION
3.1
OVERVIEW
3.1.1 STRIP WASH MODE
This mode soaks each row as it is
washed, before moving on to the next
row, and is typically used when the
soak times are short (0 - 80 seconds).
STRIP WASH MODE begins by moving to row 1 and aspirating any liquid that is in the
microplate wells. The instrument then dispenses wash solution into row 1, lets the row soak
for the entered SOAK SECONDS, and then aspirates the wash solution from the wells. The
dispense/soak/aspirate operation is repeated for the number of CYCLES entered. Each row of
the microplate is washed in turn, until the number of ROWS specified have been washed.
When soaking, the display shows the remaining soak time. Don't disturb the instrument unless
you wish to cancel the wash.
3.1.2 PLATE WASH MODE
This mode allows a row to soak while
subsequent rows are being washed,
and is used when longer (up to 999
seconds) soak times are required. The
shortest soak time is typically 80
seconds for 12 rows.
PLATE WASH MODE begins by moving to row 1 and aspirating any liquid that is in that row of
microplate wells. The instrument then dispenses wash solution into row 1, and moves to the
next row, leaving wash solution in row 1 to soak. This sequence is repeated for each row until
the number of ROWS selected have been processed. The instrument then returns the
manifold to the standby row until the SOAK SECONDS for the first row has elapsed. The
aspirate and dispense is then repeated across the microplate, completing the first wash
CYCLE. This sequence of passes across the microplate is repeated for the number of
CYCLES selected. At the end of the last CYCLE, wash solution is aspirated from the wells. All
rows are soaked for the same length of time.
Each row is left with wash solution to soak for the designated SOAK SECONDS. The
minimum soak time is determined by the number of rows and the dispense volume selected.
The maximum SOAK SECONDS is 999 seconds.
When soaking, the display shows the remaining soak time. Don't disturb the instrument unless
you wish to cancel the wash.
3.1.3 PRIME
Priming the plate washer will prepare it for operation by filling the dispense pump, tubing and
manifold with liquid and removing air from the system. Prime is used when the instrument is
first set up, fluid reservoirs have been changed, the dispense module or manifold has been
changed, or when performing a distilled water rinse from the Rinse reservoir. See section 6.1
for Prime Cycle volume usage.
Press
to start. Press
to cancel. During priming, the display will show "Pri".
Prime
Start
Stop
PROGRAM NUMBER
DISPENSE VOLUME
DISPENSE SPEED
SOAK SECONDS
WASH CYCLES
STRIP/PLATE WASH MODE
PLATE SELECTION
2-8 PORT MANIFOLD
12 PORT MANIFOLD
8 PORT MANIFOLD
PROGRAM NUMBER
DISPENSE VOLUME
DISPENSE SPEED
SOAK SECONDS
WASH CYCLES
STRIP/PLATE WASH MODE
2-8 PORT MANIFOLD
12 PORT MANIFOLD
8 PORT MANIFOLD
P L AT E S E L E C T ION
Multiwash III
14
Содержание 8441 series
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Страница 14: ... FINAL ASPIRATION WELL POSITION TOP OF WELL ASPIRATION POSITION Multiwash III 9 ...
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