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1.5.2 HORIZONTAL MANIFOLD TO MICROPLATE ADJUSTMENT
The best aspirate performance (leaving the minimum liquid in the plate wells) is obtained when
the manifold aspiration needles are close to the left side wall of the well (flat bottom wells).
Calibrating the manifold position can compensate for variations in microplate products from
different manufacturers. Repositioning the manifold aspiration needles to the recommended
.02" (.5mm) from the left-side well wall (flat bottom microplate), is completed as follows. See
examples of the proper position shown on the next page for the various manifolds available.
1. Place the microplate to be used in the plate-holder. The 8 or 12 row microplate positioning
must match with 8-Port or 12-Port manifold selected for use.
2. Press and hold
. Press the button for about 4 seconds until the instrument beeps
and shows “CAL” on the display.
3. Press
. The display will show “FLA” to indicate that you are entering the horizontal
adjustment mode. The horizontal adjustment is being made relative to the standard flat
bottom microplate configuration. This adjustment will also change the position of the
manifold for the round or V-bottom style microplate Plate Selection mode, see Section 3.3.8
for programming information.
4. Press
and the manifold will rise and move to the factory defined adjustment row as
shown in the examples. The display will show the number “820”. This number represents
the factory default position of the manifold inside the microplate well.
5. Press
to move the manifold to the right, or
to move it to the left. Each press of
the button will produce approximately .002" (.05mm) movement of the manifold to the right
or left. The display will add or subtract 1 count for each press of a button. The adjustment
range is ± 25 steps.
EXAMPLE: If the aspirate needles are determined to be too near the microplate well wall,
press
while observing needle positioning. If
is pressed five times, the needle
will move approximately .010" (.25mm) to the right.
6. Press
to accept this setting, and to return the manifold to the soaking microstrip
wells.
7. Press
again to exit the adjustment menu or press the
again to repeat the
horizontal manifold adjustment routine.
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Multiwash III
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Содержание 8441 series
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Страница 14: ... FINAL ASPIRATION WELL POSITION TOP OF WELL ASPIRATION POSITION Multiwash III 9 ...
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