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Thermo Fisher Scientific
Operation Manual HM550
• Afterwards, the chuck together with the specimen is inserted with
the peg into one of the four front fast freezing stations (fig. 111).
ese four front fast freezing stations are equipped with a Peltier
cooling element.
Integrated system for nebulization of disinfectant (Sanosil®) into the
cold cryostat chamber
During Cold D, the disinfectant (Sanosil®) is distributed throughout
the cryostat chamber. In the following chapter “disinfectant” stands for
the exclusive use of Sanosol®; use of other products may not prove as
efficacious.
e disinfectant is introduced to the chilled chamber by ultrasonic
nebulization of the disinfectant solution (Sanosil®). e droplet size of
the disinfectant mist is 1 to 3 µm. ese droplets ensure an even distri-
bution of the disinfectant throughout the entire cryostat chamber.
e Cold D process takes 50 minutes to complete, allowing disinfection
to be carried out during mormal operation of the cryostat.
e cryostat cannot be used in the 50 minutes while disinfection is
carried out, but you can continue working safely with the unit as soon
as Cold D has been completed.
Universal Precautions
All specimens can be potentially infectious; because of this universal
precautions should always be followed when handling cryotomy speci-
mens and cryostats.
Definition of Disinfection
Disinfection is the destruction of pathogens by physical or chemical
means. It is not as fully effective as sterilization. Disinfection destroys
most recognized pathogens, but not necessarily all microbial forms,
such as bacterial spores.
Cold D
(Option DC) function
Figure 111.
Peltier element.
Operating instructions
Cold D (Option DC)
Disinfection Procedure
