125
Thermo Fisher Scientific
Operation Manual HM550
e Cold D (Option DC) is a fumigation unit for the application of
disinfection media (Sanosil®) onto the operation areas in the cryo cham-
ber which are accessible for the user during the intended use.
Please refer to the
Recommended Disinfection Procedure
in
Appendix A
at the end of this manual.
W
arning
To guarantee the functionality and reliability of the fumigation unit, it
is recommended to solely use Sanosil®. When using other disinfection
media, ermo Scientific Microm International GmbH assumes no li-
ability concerning functionality and disinfection effectiveness.
Chapter 5
Working with the cryostat
Before sectioning, the specimen clamping and the cryo chamber should
be at a stable temperature around the desired cutting temperature.
Select a chamber and specimen temperature corresponding with the
consistency of the specimen that should be sectioned.
All tools which are necessary to take off sections or to manipulate the
specimen must also be cooled, as the section will stick to them. For this
reason, the required working accessories should always be stored on the
shelves or brush tray in the microtome chamber.
e heated sliding window should be closed during breaks and while
preparing work to avoid warm air getting into the chamber. Conse-
quently, more favorable work conditions with a lower frost built-up on
the microtome and knife are achieved.
In case the instrument is equipped with a Vacutome, please note that
both the coarse filter and the micro filter are properly inserted in the
filter unit. When needed, replace them and dispose of them according
to the respective lab regulations.
Cold D (Option DC)
Preparations on the
microtome and inside
the cooling chamber
Working with the cryostat
Preparations on the microtome and inside the cooling chamber
