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145

Thermo Fisher Scientiﬁ c

Operation Manual HM550

tion media,  ermo Fisher Scientiﬁ c Microm International GmbH as-

sumes no liability concerning functionality and disinfection eﬀ ectiveness.

Apendix B

Appendix B

Certﬁ cate

Microm International GmbH
Mr Benjamin Hoffmann
Robert-Bosch-Straße 49
69190 Walldorf
Germany

CERTIFICATE

Inactivation of bacteria and other pathogens

by cold disinfection in the cryostat Microm HM550

1. Summary

The cold disinfection procedure in the cryostat Microm HM550 involves fogging of a chemical
disinfectant throughout the working space of the cryostat at temperatures down to -20 °C.

The efficacy of microbial inactivation was determined in analogy to the standard methods of the
DGHM (German Society for Hygiene and Microbiology) [1] and the European standard EN
13697:2002-01 [2] for quantitative non-porous surface testing for the evaluation of chemical
disinfectants with adaptations to the test item. The instrument temperature during the tests was
set to -20 °C.

“Sanosil® S010 spezial” (SANOSIL Service GmbH, Farchant, Germany) containing
approximately 5 % hydrogen peroxide and 50 mg/L silver nitrate was used as a disinfectant.

By employing a 15 min fogging period (corresponding to 54 g Sanosil®) followed by an
additional 35 min contact time, a 99.5 to 99.999 % destruction of vegetative test bacteria was
demonstrated. The yeast

Candida albicans

was inactivated by 99.98 %.

Deposition of silver from the disinfectant onto the working space surfaces has an additional long-
term bacteriostatic effect [3].

The cold disinfection procedure thus offers a safe and convenient way to significantly reduce
microbial contamination in the cryostat working space and, therefore, infection risk.

However, the cold disinfection system cannot replace the safety precautions, regular cleaning
and disinfection of the cryostat chamber according to the manufacturer´s instructions.

17 December 2010

Ingo Maier, PhD

eco

scope

does not accept any responsibility for misleading citations due to incomplete

reproduction of this certificate. The certificate is valid only for the tested instrument type and the
testing conditions.

Hochgratweg 12
D-88279 Amtzell

Geschäftsführer:

Dr. Ingo Maier

USt-IdNr. DE223096760

FA Wangen

St.-Nr. 91306/20667

Kreissparkasse Ravensburg

Kto. 292 485 BLZ 650 501 10

IBAN DE19 65050110 0000292485

BIC SOLADES1RVB

Postbank München

Kto. 639 381 801 BLZ 700 100 80

IBAN DE54 70010080 0639381801

BIC PBNKDEFF

Labor für Mikrobiologie und Ökotoxikologie

Priv. Doz. Dr. Ingo Maier

Hochgratweg 12

D-88779 Amtzell, Germany

Tel +49 (0)7520 953 660

Fax +49 (0)7520 953 661

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Содержание Thermo Scientific Microm HM550 MV

Страница 1: ...1 Thermo Fisher Scienti c Operation Manual HM550 Part of Thermo Fisher Scienti c...

Страница 2: ...02 Thermo Fisher Scienti c Operation Manual HM550...

Страница 3: ...03 Thermo Fisher Scienti c Operation Manual HM550 Microm HM550 Thermo Scienti c CRYOSTAT MICROTOM incl options O M V P D Operation manual English ex Ser No 46320...

Страница 4: ...entative is Operation Manual may not in whole or in part be copied photocopied reproduced translated or con verted to any electronic or other form without prior written consent of ermo Fisher Scienti...

Страница 5: ...05 Thermo Fisher Scienti c Operation Manual HM550 Figure 1 Cryostat Thermo Scienti c Microm HM550...

Страница 6: ...is expressed or im plied Unauthorized modi cation or repair by third party persons will void the warranty e warranty will expire in case of improper or wrong use of the instru ment and in case the wa...

Страница 7: ...tat i e placing the specimen onto a chuck sectioning and trans ferring sections onto a slide e listed and marked safety measures as well as the regulations and hygiene measures of your respective lab...

Страница 8: ...en coarse feed 40 Speed of the specimen coarse feed 41 Setting the cutting window 42 Automatic approach system 43 Trimming and rst cuts 46 Fine feed 46 Turning on o the function retraction 47 Indicati...

Страница 9: ...unit 88 Setting the suction window 94 Knife carrier for Vacutome 95 Disposable blade carrier EV 95 Magnetic disposable blade carrier MV 98 Option P active deep freezing device with Peltier element 10...

Страница 10: ...l 130 Maintenance and care of the cryostat 132 Shutting o for cleaning 132 Removing the microtome 133 Cleaning and care of the microtome 136 Cleaning the cooling lamella 137 Changing the uorescent lam...

Страница 11: ...ine use of the HM550 Series is easy and safe if the instructions in this manual are being observed Note Special instructions regarding operation of the instrument WA I S F CA TIO general danger spot T...

Страница 12: ...ument grounding T T T I E C IEC caution mains voltage N C U danger in explosive environment T hazard of frostBite A hazard of radioactive radiation W W A R hazard of infection U A Biohazard S F I L B...

Страница 13: ...ti c Operation Manual HM550 hazard of malfunction T T I Warning of unhealthy or irritating suBstances D D F T care in using microtome knife T I B U N N N T Waste disposal A D Safety Precautions Warnin...

Страница 14: ...s Rotary Microtome with backlash and maintenance free cross roller bearings in stainless steel Electro mechanical feed system Section thickness setting from 1 to 100 microns up to 10 m in 1 m incremen...

Страница 15: ...al of sections when trimming e system consists of a duo lter system e coarse lter collects the section waste e micro lter with a 99 99 ltration e ciency for 0 1 m particles lters the air e coarse lter...

Страница 16: ...m from 20 to 70 m 10 m from 70 to 100 m Trimming thickness range 5 to 500 m Resolution 5 m from 5 to 10 m 10 m from 10 to 100 m 20 m from 100 to 200 m 50 m from 200 to 500 m Specimen retraction durin...

Страница 17: ...Handwheel brake electromagnetic in any position Emergency stop via handswitch or foot pedal Option V max specimen size 28 mm max section thickness to be disposed of max 40 m max section thickness to b...

Страница 18: ...0 V 50 Hz 6 A 10 Internal protection Primary circuits Transformer T8AH 100 115 V T4AH 220 240 V Illumination T0 8AH 100 115 V T0 4AH 220 240 V Secondary circuits Window heating T2 5AH Vacutome valve o...

Страница 19: ...Button TRIM FEED 5 Arrow button UP 6 Arrow button DOWN 7 LC display 8 Reset button 9 Menu button 10 enter button within the menu settings scroll button outside the menu settings 11 Information line 1...

Страница 20: ...Scienti c Operation Manual HM550 Figure 3 Keypad left 1 Button cutting window 2 Button ACA 3 Button coarse feed forwards 4 Button coarse feed backwards 5 Button TRIM 4 3 1 2 5 Introduction Technical d...

Страница 21: ...ad right 1 Button operating modes 2 Operating knob 3 Button START STOP 4 Button START STOP 5 Emergency stop LED 6 Button to activate the hand wheel brake 7 Brake LED 8 Button to loosen the hand wheel...

Страница 22: ...22 Thermo Fisher Scienti c Operation Manual HM550 Frontal View ear View Introduction Technical data HM550 Series...

Страница 23: ...23 Thermo Fisher Scienti c Operation Manual HM550 Lateral View Left Side Lateral View ight Side Introduction Technical data HM550 Series...

Страница 24: ...acking Remove the upper wooden cover g 5 1 e hinges of the upper wooden cover are secured with tape Remove this tape Lift the packing g 5 2 over the instrument Remove the upper and lower supporting fo...

Страница 25: ...e setting feet of the cryostat is packed together with the accessories of the cryostat separate carton on the foot rest of the cryostat Turn the setting feet completely upwards via this tool so that t...

Страница 26: ...ap prox 30 position Choose installation site that enough ventilation for the cooling system is guaranteed the distance between wall and rear panel is approx 15 cm For this install the distance bolts...

Страница 27: ...e unit tighten the screws g 6 1 by using the attached tool Warning Instead of the hand wheel handle a transportation screw g 6b 1 is inserted into the hand wheel is way the hand wheel is tightly and s...

Страница 28: ...n Insert coarse lter into the lter unit caution Section waste is collected in the coarse lter It must be replaced at least once a week depending on the amount of debris and nature of the specimens e c...

Страница 29: ...ing Before turning on the instrument for the rst time please check if the power requirements indicated on the type plate g 8 correspond to the power supply voltage being used No other instruments shou...

Страница 30: ...C In case the instrument is equipped with an optional specimen cooling the specimen temperature is pre set to 10 C e cooling phase will take approx 2 to 3 5 h depending on the set chamber temperature...

Страница 31: ...so from a greater distance When the instrument is not operated for another hour the cryostat switches to the sleep status e display illumination goes o However the equipment on indicator g 2 1 then li...

Страница 32: ...o operate the instrument easily and comfortably with a few buttons only please note the following After having chosen a function its pre selected values can be changed via the up or down buttons Howev...

Страница 33: ...he distance to the end position of the specimen To lock the keyboards and to block the feed movement press the but tons g 12 8 and 12 10 for approx 2 sec e display g 13 shows LOCKED To unlock the keyb...

Страница 34: ...the specimen clamping is retracted electro mechanically is is shown by the letter R in the status line on the display Warning When the motor drive option M for the cutting movement has been turned on...

Страница 35: ...d section or trimming thickness via button up g 16 5 or down g 16 6 e altered values are shown on the display OMVPD Figure 15 Setting section thickness and trimming thickness 5 4 2 1 3 6 Figure 16 Cen...

Страница 36: ...of the display the actual value is also shown graphically Press button g 18 3 for chamber temperature CHAMBER is then shown inverted e set value settings are made via the up button g 18 5 down button...

Страница 37: ...en chucks with a size of 50 55 60 and 70 mm Special sizes on request Put the warm chuck into the hole g 19 1 of the fast freezing rail Put freezing medium onto the chuck Place fresh tissue onto the st...

Страница 38: ...pecimen Note Use both a frozen cryo mold and a frozen heat extractor Insert the heat extractor g 20b into the fast freezing rail g 20b 1 To pre cool the heat extractor turn the knob g 20b 2 to lower t...

Страница 39: ...39 Thermo Fisher Scienti c Operation Manual HM550 4 2 1 3 5 Figure 20a Heat Extractor 2 1 3 4 Figure 20b Heat Extractor Operating instructions Chamber cooling...

Страница 40: ...the coarse feed turns o e LED in button g 21 4 blinks which means that the speci men clamping is in its rear end position Press button g 21 5 Brie y press this button to carry out a forward travel of...

Страница 41: ...reached its front end position e speed for the specimen coarse feed can be selected in three di erent settings e selected setting is shown in the status line on the display by one two or three little...

Страница 42: ...you return into the list of submenus e current setting of the submenu is also chosen and will still be valid To quit the submenu press the menu button g 23 9 Now continue working with the selected set...

Страница 43: ...ecimen the set limits are applied to the cutting movement accordingly Note In case the cutting window limits are set incorrectly please repeat above mentioned process again Note If no cutting window i...

Страница 44: ...e range Use the hand wheel to make sure that the most protruding point of the specimen is opposite the knife edge is position must be within the selected cutting window Starting the automatic approach...

Страница 45: ...specimen has been oriented again Error code AMPLIFIER ERROR is shown on the status line on the display in case the activating sig nal for the automatic approach has already been recognized before the...

Страница 46: ...the display Press the arrow buttons g 26 5 or 26 6 to select the desired trim ming value Turn the hand wheel in a clockwise direction to carry out the trim ming feed in the upper reversal point of the...

Страница 47: ...submenus Via the arrow buttons g 28 5 and 28 6 select the submenu ret raction ON OFF e selected submenu is now shown inverted Via the enter button g 29 10 con rm the submenu and simulta neously open...

Страница 48: ...s the scroll enter button g 30 10 to show the various functions one after the other Note Within the menu the scroll enter bu tton is used to con rm submenus and or settings During operation i e outsid...

Страница 49: ...ter each downward movement of the specimen holder the num ber on the section counter increases by 1 e counter can be reset to zero via button g 30 8 is value shows the sum in microns of the sections a...

Страница 50: ...reases the closer the specimen holder moves towards the front Note If no further feed is possible anymore the display automatically shows the re maining travel independently which information on the s...

Страница 51: ...38 10 con rm this submenu and simulta neously open it To change the instrument s real time at intervals of one minute press the arrow buttons g 37 5 or 37 6 If the buttons are being pressed for more t...

Страница 52: ...the list of submenus If the setting is not con rmed via the enter button you will quit the submenu automatically after ve seconds and you return into the list of submenus e current setting of the subm...

Страница 53: ...ime at intervals of 15 minutes press the arrow buttons g 41 5 or 41 6 Con rm with enter g 42 10 Note You return immediately to the list of submenus If the setting is not con rmed via the enter button...

Страница 54: ...ay WAKE Time adjusted Press the menu button g 46 9 is way you get into the list of the submenus Via the arrow buttons g 45 5 and 45 6 select Active Time is submenu is now shown inverted Via the enter...

Страница 55: ...button g 46 10 Note You return immediately to the list of submenus If the setting is not con r med via the enter button you will quit the submenu automatically after ve seconds and you return into the...

Страница 56: ...between day month and year press the reset button g 50 8 to set the correct date Via the arrow buttons g 49 5 and 49 6 change the desired eld of the date in steps of day month or year Con rm it via t...

Страница 57: ...ess the menu button g 50 9 Now continue working with the selected settings Figure 47 Display Adjust Date Ver 2 09 Figure 48 Display Date adjusted 5 4 2 1 3 6 Figure 49 Center keyboard left buttons Ope...

Страница 58: ...intervals of 15 minu tes press the arrow buttons g 53 5 or 53 6 Con rm with enter g 54 10 Note You return immediately to the list of submenus If the setting is not con r med via the enter button you w...

Страница 59: ...ng with the selected settings Note Defrosting can only be carried out at the set defrosting time if the real time is set correctly see page 51 Figure 52 Display Defrosting time adjusted 5 4 2 1 3 6 Fi...

Страница 60: ...inverted Via the enter button g 58 10 con rm this submenu and simulta neously open it Via the arrow buttons g 57 5 or 57 6 choose ON or OFF If ON is con rmed request defrost is shown in the info line...

Страница 61: ...y necessary to remove the section waste as well as the tissue Warning Remove the specimen from the chamber when defrosting is carried out e temperature inside the chamber rises and thus the tissue spe...

Страница 62: ...the arrow buttons g 59 5 or 59 6 select ON or OFF ON is selected when defrosting is to be interrupted Via the enter button g 60 10 con rm the setting 5 4 2 1 3 6 Figure 59 Center keyboard left button...

Страница 63: ...again at the pre selected defrosting time Note While INT is active neither the defrosting time nor the real time can be changed When INTR ACT appears in the status line this means that INTER RUPT was...

Страница 64: ...the menu button g 65 9 is way you get into the list of submenus Via the arrow buttons g 64 5 and 64 6 select contrast is submenu is now shown inverted Via the enter button g 65 10 con rm this submenu...

Страница 65: ...9 Now continue working with the selected settings 5 4 2 1 3 6 Figure 64 Center keyboard left buttons 8 9 10 Figure 65 Center keyboard right buttons e information on the display can be shown in four di...

Страница 66: ...English Deutsch Espanola Fran ais Via the enter button g 69 10 con rm the setting Note You return immediately to the list of submenus If the setting is not con rmed via the enter button you will quit...

Страница 67: ...instru ment is turned on e lamp remains on as long as the instrument is in its active status see ACTIVE Time page 51 Used lamps can be changed by the user himself see page 139 Turning the light on of...

Страница 68: ...re 71 Center keyboard left buttons 8 9 10 Figure 72 Center keyboard right buttons Figure 70 Display Illumination of cooling chamber Ver 2 09 Service settings Note Menu service settings are only releva...

Страница 69: ...7 Via the orienting lever g 74 6 the specimen clamping can be moved in all directions by approx 8 After having achieved the desired alignment x the position via the clamping lever g 74 7 e orienting l...

Страница 70: ...4 0 1 6 1 4 3 5 9 8 7 1 6 2 2 1 Inserting the knife e knife is inserted into the knife carrier either from the side or from the front independently if one or two clamping plates g 75 1 have been remo...

Страница 71: ...e height of the knife to the position of the anti roll device Note Please note the parallel alignment of knife edge and edge of the anti roll plate Afterwards tighten the clamping screws g 75 2 to x t...

Страница 72: ...carrier EC 5 7 8 6 4 3 10 9 1 2 11 Figure 76 Disposable blade carrier EC Inserting the blade e blade is inserted into the slot behind the clamping plate Note When using high pro le blades rst loosen...

Страница 73: ...ed solidly into the blade carrier e com plete carrier is moved Open the clamping lever for the upper part of the carrier g 76 2 and move the blade together with the upper part to the left or right sid...

Страница 74: ...another version it takes also up high pro le blades e anti roll plates have a length of 69 5 mm Inserting the blade e blade is inserted into the slot g 77 9 behind the clamping plate Note When using h...

Страница 75: ...of the blade is no longer usable the blade carrier can be moved e blade itself is clamped solidly into the blade carrier e com plete carrier is moved Open the clamping lever for the upper part of the...

Страница 76: ...ades with the dimensions 80 x 19 mm and or 60x19 mm and a facet angle between 24 and 30 Inserting the blade Note Special ermo Scienti c magnetic blades must be used only Insert the blade into the blad...

Страница 77: ...ier Swivel the upper part of the blade carrier on the base until the desi red clearance angle is achieved e clearance angle can be read on the scale on the left side of the base Again tighten the clam...

Страница 78: ...from 10 C down to 50 C e actual value of the specimen temperature is shown as actual temperature and as a graphic After having chosen the requested values the display automatically returns to its basi...

Страница 79: ...lamping lever g 81 2 towards the front e entire specimen head can now be oriented manually together with specimen cooling if equipped in all directions by approx 8 After having achieved the desired al...

Страница 80: ...proportionally higher retraction speed is carried out To adjust the cutting window in relation to a specimen it can be set continuously within the maximum values 60 mm and can be adjusted to the respe...

Страница 81: ...on the display Note A cutting window should only be set while the specimen is moved down wards Note In case the cutting window limits are set incorrectly please repeat above mentioned process again 4...

Страница 82: ...isplay To start a continuous cutting cycle use the foot pedal or press the two start buttons g 85 3 and 85 4 To stop the continuous stroke press foot pedal or one of the two start buttons g 85 3 or 85...

Страница 83: ...knob g 86 2 Note To save time the return travel speed is enhanced in relation to the cutting speed especially for slow cutting speeds Press the two buttons g 86 3 and 86 4 of the safety switch either...

Страница 84: ...nd wheel brake should be turned on when working on the specimen holder or knife carrier Stop the motorized cutting movement via buttons g 87 3 and 87 4 en activate the function hand wheel brake via bu...

Страница 85: ...ncy stop e hand emergency stop g 89 1 is located above the right arm rest Press this button to immediately stop the motorized cutting drive When the hand emergency stop is activated the red LED g 88 5...

Страница 86: ...in the foot pedal g 91 Vigorously step on the foot pedal to immediately stop the moto rized cutting drive However the LED g 88 5 does not light up when the foot emergency stop is pressed Foot emergenc...

Страница 87: ...uction power Note e user sets the desired value depending on the desired function stretching or suction process section thickness and size of the specimen Note e applied vacuum for stretching sections...

Страница 88: ...the coarse lter downwards either manu ally or via the foot e coarse lter tilts forwards Remove the coarse lter by means of the attached clamp for lter replacement e coarse lter must be replaced depen...

Страница 89: ...according to the respective lab regulations Note e coarse lter is to be understood as a disposable lter and must not be used again Insert new coarse lter and close the lter Note In case section waste...

Страница 90: ...the arrow buttons g 94 5 and 94 6 reset the sum of sections since the last resetting to 0 m Via the enter button g 95 10 con rm the setting Note You return immediately to the list of submenus If the s...

Страница 91: ...r if the message COARSE FILT appears on the display the lter must be replaced Depending on how often the instrument is used the micro lter must be replaced at regular intervals however at least every...

Страница 92: ...his setting and simultane ously open it Via the arrow buttons g 97 5 and 97 6 reset the sum of sections since the last resetting to 0 m Via the enter button g 98 10 con rm this setting Note You return...

Страница 93: ...rnal counter for the micro lter it is possi ble to ignore the message CHANGE MICRO FILT for the moment For this press the scroll enter button g 98 8 However this me ans that the counter must still be...

Страница 94: ...of the specimen just below the knife edge Press button g 100 1 to set the lower limit of the suction win dow e LED in button g 100 1 lights up during each further passing through of the suction windo...

Страница 95: ...mmercially available low pro le blades with a dimension of 80 x 8 mm as well as high pro le blades with a dimension of 76 x 14 mm an a facet angle of approx 35 Knife carrier for Vacutome Disposable bl...

Страница 96: ...ing lever g 101 2 Note Press the clamping plate on its lower area to open the slot behind the clam ping plate Push the blade from the left side into the slot behind the clamping plate g 101 1 as far a...

Страница 97: ...hieved at a clearance angle of 10 or more To move the blade carrier loosen the lever on the right side of the base plate To x the blade carrier in its new position clamp the lever again e vacuum anti...

Страница 98: ...Scienti c magnetic blades must be used Insert the blade g 103 1 into the blade slider g 103 2 and put it on the magnetic surface g 103 2 of the blade carrier from the side Note To move the blade to t...

Страница 99: ...r side of the blade caution To avoid the danger of injury on the blade during adjustment of speci men always position the knife guard g 103 10 over the blade edge Loosen the clamping lever g 103 7 Swi...

Страница 100: ...e ects For this the closed vacuum anti roll hood g 103 4 slightly tilts the underneath ap g 104 8 via the stud screw g 103 11 thus opening a suction slot g 104 9 Lift the vacuum anti roll hood g 104...

Страница 101: ...eltier functions are turned o automatically after 8 min To active or deactivate the fast freezing function press button g 107 2 for 1 sec g 109 Ver 2 09 Figure 105 Display Fast freezing station Figure...

Страница 102: ...end temperature is way tissue specimens can be detached from the chuck inside the chamber g 110 First put freezing medium onto an non cooled chuck en put the specimen onto the freezing medium OMVPD O...

Страница 103: ...oplets ensure an even distri bution of the disinfectant throughout the entire cryostat chamber e Cold D process takes 50 minutes to complete allowing disinfection to be carried out during mormal opera...

Страница 104: ...thus o ers a safe and convenient way signi cantly reduce microbial contamination in the cryostat working space and therefore infection risk However the Cold D system cannot replace the safety precauti...

Страница 105: ...to the disinfectant tank on the back of the unit Fig 112a Before lling the tank check the shelf life and the hazardous substance warnings on the disinfectant Sanosil Also always follow the manufacture...

Страница 106: ...l container Note To ensure that the Cold D system can function properly always adhere to the sequence of operations speci ed above Warning To avoid the frosting of the chamber the Cold D should not be...

Страница 107: ...e submenus listed Next use the arrow keys Fig 114 5 and 114 6 to select the OPT DC NOW submenu from the list Fig 115 Press Enter Fig 113 10 to con rm CAUTION DO NOT OPEN CRYO CHAMBER DURING THE COLD D...

Страница 108: ...use the arrow keys Fig 115 5 and 115 6 to select the re quired submenu from the list Press Enter Fig 113 10 to open the selected submenu Select the required operation Fig 114 5 and 114 6 and press Ent...

Страница 109: ...n the submenu Use the arrow keys Fig 114 5 and 114 6 to select between Weekly and Daily in this submenu Press Enter Fig 113 10 to con rm If the Daily mode is selected Fig 118 the Cold D cycle runs dai...

Страница 110: ...rs between the beginning of the defrosting cycle and the next Cold D cycle If this delay is ignored when programming the Cold D time an error message is displayed and the Cold D time is automatically...

Страница 111: ...selected in the OPT DC MODE submenu enter the day of the week on which you wish Cold D to be performed in this submenu Use the arrow keys Fig 114 5 and 114 6 to scroll through the days from Monday to...

Страница 112: ...9 If the Daily cycle has been selected in the OPT DC MODE submenu you can select whether you wish Cold D to be performed at the weekend in this submenu Use the arrow keys Fig 114 5 and 114 6 to select...

Страница 113: ...114 6 to enter the date on which you wish the expiry date reminder to be displayed in the six digit date box day month year Press the Reset button Fig 113 8 to switch between day month and year Press...

Страница 114: ...national GmbH Four di erent error codes which indicate a defect on a temperature sensor can appear on the display e message is shown on the second line from below on the display g 126 e instrument rec...

Страница 115: ...and contact a service technician ere is insu cient disinfection medium in the container Please check and re ll disinfection medium if necessary Restart the Cold D It might be possible that a repeatedl...

Страница 116: ...ing Please turn o the motor and restart the Cold D e Cold D cycle can not be started as long as defrost is activated Please wait at least for two hours after the defrosting is completed and restart th...

Страница 117: ...334170 1 Center grip 424210 1 cryostat oil no 407 100 ml 350040 1 freezing medium 118 ml 161425 Section waste tray for instruments without specimen cooling consisting of 1 Abfallwanne 415600 1 Zwisch...

Страница 118: ...m 570390 Knife carriers Standard knife carrier 705460 Disposable blade carrier EC 705470 Magnetic blade carrier 705250 Knife carriers for option Vacutome Disposable blade carrier EV 705580 Magnetic bl...

Страница 119: ...hamber temperature is fast freezing station has 12 positions for one chuck each e instrument can option ally be equipped with an active deep freezing device e station can be cooled down to 60 C by mea...

Страница 120: ...vaporator becomes necessary To carry out the defrost ing cycle a heating warms the evaporator from below Set the time of the daily defrosting on the control panel e period of the defrosting takes appr...

Страница 121: ...cted section thickness is delivered at the upper reversal point of the return travel e number of the sections made can be shown on the section counter on the display After each downward movement of th...

Страница 122: ...be lower than 35 C when activating the ACA In case the temperature is lower than 35 C it is recommended to insert the chuck into the specimen clamping and wait until the specimen tem perature has adj...

Страница 123: ...on to the next lower reversal point When in operating mode continuous stroke an unlimited number of sections is carried out To stop the continuous stroke press the button START STOP or the foot pedal...

Страница 124: ...for a more e cient working with the cryostat For various specimens di erent cut ting temperatures can be achieved very quickly ere is the possibility to rapidly freeze on the specimen at 50 C on a chu...

Страница 125: ...and specimen temperature corresponding with the consistency of the specimen that should be sectioned All tools which are necessary to take o sections or to manipulate the specimen must also be cooled...

Страница 126: ...equipped with a heat extractor additional equip ment the specimen can be frozen faster and more evenly Put the pre cooled heat extractor onto the specimen from above is way the specimen is frozen at t...

Страница 127: ...f the buttons on the operating controls Put the anti roll plate against the knife by means of the knob Turn the hand wheel in a clockwise direction or use the motorized cutting drive to carry out sect...

Страница 128: ...a clearance angle of 8 12 according to the facet angle of a steel knife Select a proper cutting speed e harder the material the slower the cutting speed Take care in bringing specimen and knife toget...

Страница 129: ...other unexpected malfunctions of the instrument the specimen might be damaged How to avoid malfunctions when working with the Vacutome Assure the opening of the Vacutome ap via a correct adjustment of...

Страница 130: ...adequately clamped Check knife clamping Chuck inadequately clamped Check clamping Specimen inadequately frozen on onto the chuck Again freeze on the specimen Specimen grossed too thick it loosens fro...

Страница 131: ...roll plate Thick thin sections Knife sharpness Move the knife Knife angle Readjust Clamping on knife carrier Check clamping Clamping on specimen holder Check clamping Tight hand wheel punctual Debris...

Страница 132: ...o in its upper position Remove the middle and rear section waste tray Dispose of the sec tion waste according to the respective lab regulations Remove and or suck o cold section waste If the instrumen...

Страница 133: ...ouch the connectors on their connector housings Insert the blue coded connector g 129 1 into the park position 9 g 129 9 of the fast freezing station Insert the red coded connector g 129 2 into the pa...

Страница 134: ...ger of freezer burns on the frozen microtome Move the microtome to the left side until the stop is way the clutch on the right side of the microtome becomes free For the option O pull o the quick coup...

Страница 135: ...ly clean and wash the interior caution Chloric cleaning agents must not be used for cleaning purposes In case of a malfunction hazardous gases might be generated together with the cooling brine Tyfoxi...

Страница 136: ...ty inside the microtome dry the microtome components inside the cooling chamber very carefully For this the microtome can be treated in a drying closet at tempera ture up to 60 C After each shutting o...

Страница 137: ...egular and or immediate disinfection of the blade carrier the cryo chamber as well as all the other potentially contaminated parts of the instrument is highly recommended All commercially available cr...

Страница 138: ...hole is kept on the right side by a magnet g 133 2 an is inserted on the left side Remove the dust from the cooling lamella by means of a commer cially available vacuum cleaner Note Carry out this cl...

Страница 139: ...a counter clockwise direction and remove them Remove the grip g 135 1 from the upper side of the sliding win dow Turn the lamp by 90 forwards or backwards and remove it from the sockets g 135 4 Insert...

Страница 140: ...ational e contact address can be found at the beginning of this operation manual To guarantee trouble free function of the instrument after transpor tation please note the below mentioned measures for...

Страница 141: ...ith non conform packaging are not covered by the manufacturer warranty Any damage repairs resulting in non conforming package are fully charged to the sending party We reserve the right depending on s...

Страница 142: ...ion Again install the blade carrier section waste trays and the brush shelf Attach the hand wheel handle again After having turned on the instrument and after having reached the set temperature the in...

Страница 143: ...we recommend to contact a local recycling company for the disposal according to the national ap plicable regulations To be applied in the countries of the European Union and other Eu ropean countries...

Страница 144: ...loves gown mask and protective eyewear Remove all disposable blades and knives from the chamber and disinfect them separately Using a paper towel remove all tissue trimmings and waste material from th...

Страница 145: ...responding to 54 g Sanosil followed by an additional 35 min contact time a 99 5 to 99 999 destruction of vegetative test bacteria was demonstrated The yeast Candida albicans was inactivated by 99 98 D...

Страница 146: ...xide and 50 mg L silver ion complex according to the supplier Sanosil Service GmbH 82490 Farchant Germany 2 4 Test method Suspensions of test organisms were prepared in distilled water containing 0 03...

Страница 147: ...ner H P Kirsch Altena A Bansemir K Standardmethoden der DGHM zur Pr fung chemischer Desinfektionsverfahren 14 Fl chendesinfektion praxisnaher Versuch 14 1 berpr fung der bakteriziden und fungiziden Wi...

Страница 148: ...Thermo Fisher Scienti c Operation Manual HM550 Anatomical Pathology Otto Hahn Strasse 1 A 69190 Walldorf GERMANY 49 0 6227 8360 4481 Campus Drive Kalamazoo MI 49008 USA 1 800 522 7270 www thermo sher...

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