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77210163GB Issue 3

APPENDIX C
EXAMPLES

C.1 INTRODUCTION

This section provides a guideline cutting temperatures for the Thermo Cryotome.

C.2 SUGGESTED CUTTING TEMPERATURES fOR

fROZEN TISSUES

BE AwARE ThAT TISSUE SAMPLES MAY POSE A BIOhAZARD. TAKE
SUITABLE PRECAUTIONS

TISSUE TYPE

ChAMBER

TEMPERATURE

(°C)

UNFIXED TISSUE

Brain

-12

Liver

-14

Lymph Node

-14

Kidney

-16

Spleen

-16

Muscle

-20

Thyroid

-20

Skin

-25

Breast

-25

Breast with fat

-30 or below

Adipose tissue

-30 or below

FIXED TISSUE

Fixed tissue

-12 to -17

* These temperatures are guidelines only. Make sure that the tissue is sufficiently

frozen before sectioning.

Содержание Cryotome

Страница 1: ...77210163GB Issue 3 STANDARD AND ELECTRONIC OPERATOR GUIDE 77210163 Standard E ENGLISH Issue 3 Cryotome ...

Страница 2: ...ation IF THIS SYMBOL APPEARS ON THE INSTRUMENT ALWAYS REFER TO THIS OPERATOR GUIDE This symbol IS USED ONTHE EQUIPMENT OR IN A DOCUMENT To warnthat A HAZARD MAY EXIST DUETO AN EXTREME OFTEMPERATURE IFTHIS SYMBOL APPEARS ONTHE INSTRUMENT ALWAYS REFERTOTHIS OPERATOR GUIDE This symbol IS USED ONTHE EQUIPMENT OR IN A DOCUMENT To warnthaT THERE MAY BE A BIOHAZARD ASSOCIATED WITH THE INSTRUMENT ALWAYS A...

Страница 3: ...60 Warranty Statement Declaration of Conformity INDEX 62 APPENDIX A TRANSPORTATION INSTRUCTIONS 65 To Repackage the Instrument Product Return Safety Declaration APPENDIX B APPROVED REAGENT LIST 69 APPENDIX C EXAMPLES 70 Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company Cryotome Cryomatrix and Cryochrome are trade marks of Thermo Shandon Limited Thermo Fisher Scientific is the tra...

Страница 4: ...ome are marked E only for Electronic Cryotomes or Std only for Standard Cryotomes 2 SAFETY THIS PARAGRAPH DETAILS IMPORTANT SAFETY INFORMATION PLEASE READTHIS SECTION CAREFULLY Thermo products are designed for convenient and reliable operation and to accepted standards of safety The use of the Cryotome does not entail any hazard if operated in accordance with the instructions given in this manual ...

Страница 5: ...nives and blades are used in this instrument Make sure you understand the correct methods for their fixing and use viii The Centre of Gravity position for the Cryotome is shown below 221 mm 508 mm 317 mm WINDOW BOOST ix The Cryotome weighs approximately 120 kilograms 265 lbs when empty get help to move it x If the instrument has been used with materials that are toxic or contaminated with pathogen...

Страница 6: ... that a Maintenance Contract is taken out with your supplier xv The instrument must be serviced annually by a Thermo trained engineer in accordance with the instructions contained in the Cryotome Service Manual 77210166 xvi Any problems and queries should be referred to your supplier xvii Refrigeration System The Cryotome uses R404A refrigerant THE REFRIGERATION SYSTEM MUST ONLY BE WORKED ON BY SU...

Страница 7: ...rols e Temperature Controls f Keyswitch g Handwheel h Instrument Lowering Knobs i Waste Bottle j Advance rewind controls E only Micron Selector Advance rewind controls Std only For optimum performance the microtome Specimen Head and Knife Holder are held within the refrigerated chamber The main body of the microtome is mounted outside the chamber to give more space for working in and for easier cl...

Страница 8: ...nel is divided into five sections 2 2 1 MICRON SELECTOR E only The Micron Selector section of the Control Panel displays the thickness in microns of the sections being taken The number in the display is changed by pressing the and buttons Press to increase the thickness by 1 if the number is in the range 0 19 or 5 if the number is in the range 20 55 Press to decrease the thickness by the same amou...

Страница 9: ... at the end of each cutting stroke the segments of the display light one by one until eventually all the segments are lit when no more travel is available An audible alarm operates when the end of travel is reached E only Press RESET to return the Specimen Head to its maximum available travel position The button LED will light and the display will flash during the reset and the segments will count...

Страница 10: ...ess and hold DEFROST then press Display will show Day DAY 0 9 see note 1 below Hours HOURS 0 23 Minutes MINUTES 1 59 Note 1 Use the following settings for the days DAY 0 prevents the programmed function DAY 1 7 defrosting starts at the time set on the day shown set Monday as Day 1 to avoid confusion DAY 8 defrosting starts at the set time every day DAY 9 STANDBY PSAVE power save 2 Standby PSAVE al...

Страница 11: ...2 3 2 To Set Defrost Times but press FUMIGATE instead of DEFROST To start PSAVE mode press IMMED FUMIGATE The instrument then starts the 5 hour Fumigate cycle before going into PSAVE 2 2 4 CHAMBER CONTROLS CHAMBER CONTROLS CRYOBAR BOOST WINDOW LOCK LAMP CRYOBAR TEMP WORKING TEMP IMMED DEFROST IMMED FUMIGATE CANCEL DEFROST FUMIGATE TEMPERATURE C 2 2 4 1 IMMED DEFROST Press IMMED DEFROST to defrost ...

Страница 12: ...ATA SHEET msds FOR FORMALIN Note 1 The window MUST be shut and locked before fumigation can start If IMMED FUMIGATE is pressed when the window is not shut Err 4 is displayed and the fumigate cycle is aborted 2 Fumigation can only be cancelled by CANCEL DEFROST FUMIGATE during stages iii and iv that follow Stage v and vi cannot be cancelled Press IMMED FUMIGATE to initiate the following sequence i ...

Страница 13: ...ryobar for 10 minutes The compressor is also switched on for this period However if the compressor switched off within the last three minutes there is a delay of up to three minutes before the compressor re starts If CRYOBAR BOOST is pressed while its LED is flashing at the end of the boost period the boost is repeated for a further 10 minutes otherwise normal operation follows when a boost period...

Страница 14: ...ed to within 1 C of the set temperature If no temperature is set or after a reset the working temperature automatically defaults to 20 C or the last programmed temperature CRYOBAR TEMP displays the surface temperature of the Cryobar However if CRYOBAR BOOST is lit on the adjacent panel the display shows the approximate surface temperature of the peltier element in the Cryobar 2 2 6 KEYSWITCH CONTR...

Страница 15: ...oarse adjustment of the Specimen Head position and also to retract the Specimen Head to the Maximum Travel Available position Turn the Rewind Advance Control knob clockwise to rewind the head and anti clockwise to advance the head One rotation of the knob equates to a movement of 100µm at the Specimen Head 2 3 2 Cryotome E only The Advance Control Panel on the Cryotome E contains the following but...

Страница 16: ...mation in memory while the power is switched off Stored information is lost if it is allowed to discharge Power must be on to recharge the battery If LOBAT still shows after power is restored for more than 12 hours contact your Thermo dealer for service iii dEF shows when defrosting is in progress iv shows if the associated temperature probe is faulty v Fault Codes show in the Temperature display ...

Страница 17: ...Refrigerated Chamber at the time normal operation resumed Press CLOCK to clear iii P SAVE shows until the programmed time if the instrument is in Standby PSAVE mode following the selection of a DAY 9 Defrost or Fumigate function Press CANCEL DEFROST FUMIGATE to override iv Err 1 denotes an invalid push button entry or if too many push buttons are pressed simultaneously Press CLOCK to clear v Err 2...

Страница 18: ...s On Off Circuit Breaker This is the main power switch of the instrument Its use is fully described in section 3 5 b Power Inlet The mains power inlet It is fully described in section 3 4 c Window Boost Press the Window Boost switch to provide extra de misting power in conditions of high humidity Its use is described in section 5 4 ...

Страница 19: ...chase Order and that the power supply capability of your local socket outlet is compatible with the power demand of the Cryotome Move the crate near to where the instrument is to be sited Cut the retaining straps then lift the outer case vertically Read the label on the rear of the instrument and check that it conforms with your order Retrieve the accessories pack from on top of the pallet in fron...

Страница 20: ...otome turn the lowering knobs clockwise until the two front feet of the instrument are on the floor Push the instrument Alternatively two people can lift the instrument using the hand holds on either side of the instrument THE INSTRUMENT IS HEAVY ALWAYS MAKE SURE THE ROUTE IS CLEAR WHEN MOVINGTHE INSTRUMENTTO AVOID COLLISIONS 3 3 TO REMOVE THE TRANSIT FIXINGS TRANSIT FIXINGS ARE ATTACHED TO VARIOU...

Страница 21: ...the fan b and the compressor c Remove the two wood supports d coloured red from under the compressor mounting plate Make sure that the power lead from the compressor to the junction box e is securely fitted Firmly hold the blue plastic coil of the defrost valve on the transit plate at the back of the microtome then cut the ties that hold the valve f With the coil of the defrost valve still connect...

Страница 22: ...k that the detection flag at the end of the arm is in line with the slots in the two black plastic opto units of the printed circuit board j This signifies that the leadscrew and trunnion nut are located correctly Note 1 Turn the leadscrew to adjust the setting if necessary Undo and remove the transit plate that supported the defrost valve k Replace the rear panel and 8 screws Retain the transit p...

Страница 23: ...AUGHTS DO NOT SITE THE INSTRUMENT NEAR TO SOURCES OF DOWNDRAUGHTS SUCH AS WINDOWS OR HEATING VENTILATION DUCTS Turn the lowering knobs counter clockwise until the feet of the instrument are on the floor The retraction knobs can be unscrewed completely and removed if preferred Raise the front edge of the top cover remove the transit packing from the top of the sliding window then lower the cover ba...

Страница 24: ...produces a corresponding movement of the Specimen Head Turn the handwheel until the handle is at the 12 o clock position then push the lever of the lock away from you Check that the lock operates and that the handwheel cannot rotate Check that the Specimen Head is at the top of its travel Move the lock lever towards you and check that the handwheel rotates smoothly and produces vertical oscillatio...

Страница 25: ...1 The symbol on the rating plate indicates that the instrument operates on an alternating current supply a c Make sure that the I O power switch at the rear of the instrument is switched off O side of the switch pushed inward a Instruments are supplied with power cords with moulded plugs suitable for many countries If another plug is required it is necessary for a technically competent person to r...

Страница 26: ...IETARY GROUND FAULT CIRCUIT INTERRUPTION DEVICE EARTH LEAK DETECTION MAY BE FITTED AT THE WALL SOCKET AS AN ADDITIONAL SAFEGUARD 3 5 TO SWITCH ON AND OFF 3 5 1 TO SWITCH ON Press the l ON side of the l O switch inward to switch the instrument on Check that the following occur during power on i ALL lights and indicators of the Control Panel light for three seconds ii E only the Specimen Travel Avai...

Страница 27: ... less than the current temperature of the Refrigerated Chamber 3 5 2 TO SWITCH OFF Press the O OFF side of the l O switch inward to switch the instrument off 3 6 TO PREPARE THE INSTRUMENT FOR USE Practice good biological safety procedures whenever the Cryotome is used Make sure that the Waste Bottle is filled with sufficient 10 Formalin solution to cover the outlet pipe Refer to the formalin Mater...

Страница 28: ... Aerosols can form when the specimen traverses the knife These can be hazardous if inhaled Setting up the Cryotome for routine operation involves the installation and adjustment of one or more of the following components of the microtome i Knife Holders ii Knife Guards iii Standard Solid Knife iv Cutting Angle v Disposable Blades vi Disposable Blade cutting angle vii Anti Roll Plate angle viii Ant...

Страница 29: ...Holder is constructed of two parts a Top and a Base There are three types of Top to suit the types of knife or blade The same Base supports all versions of Top To fit a Knife Holder on the carrier for disposable blades shown in the diagram or a standard solid knife first pull the left hand pivoting lever of the Knife Holder fully toward you Engage the sprung dowel at the bottom of the Knife Holder...

Страница 30: ...URY IF MISHANDLED ALWAYS WEAR MESH GLOVES WHEN HANDLING A KNIFE STORE ALL KNIVES IN THEIR TRANSPORT BOXES WHEN NOT REQUIRED FOR IMMEDIATE USE ALWAYS PLACE THE PLASTIC KNIFE GUARD OVER THE CENTRE OF PORTION OF THE KNIFE WHENEVER THE INSTRUMENT IS NOT BEING USED FOR CUTTING Note 1 The stainless steel knife guard is specifically designed to operate with 160 x 34 x 10mm knife profiles Loosen the two c...

Страница 31: ...nd pivoting lever of the Knife Holder towards you Set the angle of the holder between 4 and 5 depending on the blade facet angle then lock it at that angle by pushing the right hand lever away from you Adjust the angle of attitude of the anti roll plate by lining up the datum line with the third line of the head Use an Allen key to slacken the head as necessary tighten after adjustment Note 1 If t...

Страница 32: ...i roll plate support bar to set the approximate height for the anti roll plate Loosen and tighten the Allen screw as necessary Use the height adjust control b to set the precise height of the anti roll plate You should JUST be able to see the top of the anti roll plate above the edge of the knife blade Fine adjust so that the specimen JUST misses the anti roll plate when cutting Use the nylon adju...

Страница 33: ...amp lever to the left clockwise until it is vertically down Push the end of the blade to be installed part way out of the dispenser and put its left end under the plate of the blade clamp Slide the blade to the left until its right hand end drops into the recess of the blade transporter Use the transporter to move the blade into its first cutting position then push the blade clamp lever to the rig...

Страница 34: ...ve the glass plate carefully until the top edge is parallel to the knife Hold the glass plate firmly in position and tighten up the two screws Use the Height Adjust Control a to set the height of the Anti Roll Plate relative to the knife edge Once adjusted further movement should not be required Note 1 This is a very fine adjustment If it is set too low sections will curl above the Anti Roll Plate...

Страница 35: ...n mounted The frozen specimen on the Cryocassette is held in the Specimen Head by a Quick Release Cryocassette Clamp The Clamp lever b at the top of the Specimen Head operates a moveable jaw that holds the Cryocassette firmly in position To install a cryocassette in the Quick Release Clamp i Pull the Clamp lever b towards you ii Fit the cryocassette under the two fixed jaws as shown iii Release th...

Страница 36: ...place turn the Orientation Clamp towards you to unlock the clamp Use the knurled rotary control b at the top of the Orientation Head to set the vertical orientation of the specimen Clockwise rotation tilts the specimen upward counter clockwise rotation tilts the specimen downward Use the knurled rotary control a at the left hand side of the Orientation Head to set the horizontal orientation of the...

Страница 37: ...to unlock the movement of the head Move the adjustment shaft to set the vertical and horizontal orientation of the specimen When the specimen is in the correct position tighten the knob e to lock it into position 3 6 6 4 To Change the Orientation Heads Each Orientation Head is fitted in the same way To remove the Orientation Head loosen the allen screw at the side of the Orientation Head support f...

Страница 38: ...is not lit when the Control Panel is enabled and the key cannot be removed If WINDOW LOCK is lit press the WINDOW LOCK push button once to release the window and extinguish the indicator Note 1 Performance will be adversely affected if the ambient temperature exceeds 30 C 86 F 4 2 MOUNTING A SPECIMEN ALWAYS BE AWARE THAT ANY SAMPLE MAY POSE A BIOHAZARD TAKE SUITABLE PRECAUTIONS Lower the heat sink...

Страница 39: ...gested method Take a clean Cryocassette that has been held at room temperature Apply a layer of specimen embedding medium such as Cryomatrix or Cryochrome to the grooved side of the Cryocassette then lift the heat sink sufficiently to allow you to place the Cryocassette on the Peltier platform of the Cryobar Cryomatrix and Cryochrome are proprietary Thermo consumables When the mountant starts to f...

Страница 40: ...ST ii Enable Cryobar Boost for a further 10 minutes by pressing CRYOBAR BOOST while the Cryobar Boost LED is flashing Turn the handle of the handwheel to the 12 o clock position and apply the brake Transfer the Cryocassette complete with frozen specimen from the Cryobar to the Specimen Head and secure it in position with the Specimen Clamp see 3 6 5 4 3 TO SET THE KNIFE HOLDER FOR CUTTING SPECIMEN...

Страница 41: ...n towards the knife using the Rewind Advance knob on the Advance Control Panel 4 4 TO CUT SECTIONS KEEP THE WINDOW CLOSED WHEN CUTTING OR INJURIOUS AEROSOLS MAY BE INHALED At the Micron Selector panel use the and push buttons E only or the Micron Selector Knob Standard only to set the required section thickness MICRON SELECTOR E only Std only To cut sections turn the Handwheel smoothly The instrum...

Страница 42: ...mp or Head Decontaminate and clean the Cryocassette 4 6 HOUSEKEEPING Daily Decontaminate the specimen brush by putting it overnight in a solution of 10 Formalin Wash and dry the brush each morning before use After Fumigation Carefully remove the debris tray Knife Holder and any debris Wash in running hot water initially to remove the debris then immerse overnight in a solution of 10 Formalin The f...

Страница 43: ...bvious damage whenever it is cleaned If any damage is found contact your Thermo supplier Routine maintenance by the Operator does not require the removal of any panels or fixtures Precision maintenance and adjustment should only be performed by Thermo trained Service personnel and it is recommended that a Maintenance Contract is obtained from our Service Department IF HAZARDOUS MATERIAL IS SPILT O...

Страница 44: ...ts components and accessories 5 2 FUMIGATION Carefully load the heating trough in the Refrigerated Chamber with 2 ml of concentrated formalin solution Close the window Press WINDOW LOCK and check that the push button indicator is lit To start the fumigation immediately or to program in a start time press IMMED FUMIGATE in the Chamber Controls panel Fumigation takes place at the same time every day...

Страница 45: ...fety the fumigation cycle cannot take place if the window is not closed and locked If the Cryotome is not refrozen after fumigation has taken place formaldehyde fumes will be present in the chamber Take care when opening the window 5 3 CLEANING Use a proprietary spray foam cleanser or a cloth damped in soapy water and a dry polishing cloth to periodically clean the outside of the cabinet DO NOT US...

Страница 46: ... refrigeration system Press IMMED DEFROST if the cooling coils are frosted up or if the performance of the refrigeration unit deteriorates Alternatively use the DAY 8 setting of the timer to defrost for 15 minutes at a regular time each day The temperature of the Refrigerated Chamber will not normally rise above 0 C Note 1 This cycle is used to remove the build up of small amounts of ice around th...

Страница 47: ...SUBMERGED If condensation appears on the window in conditions of high relative humidity set the Window Boost switch at the rear of the instrument to ON Note 1 The window boost facility provides extra de misting power in conditions of high humidity 2 The refrigeration system operates less efficiently if the window boost facility is left switched on unnecessarily 5 5 MAINTENANCE SWITCH OFF THE INSTR...

Страница 48: ... interrupts the electrical supply to the instrument if an internal fault occurs The trip replaces the function of a fuse When the electrical protection device trips the mains switch goes to its O position OFF and the instrument becomes inoperative If the protection circuit operates this will normally indicate a compressor problem Check that there is adequate space around the outside of the unit fo...

Страница 49: ...NSTRUMENT DO NOT ATTEMPT TO OVERRIDE THE PROTECTION TRIP Portable Appliance Testing PAT should only be carried out annually to avoid damaging the equipment Flash testing should only be carried out when absolutely necessary as damage to sensitive electronic devices may occur WARNING WARNING ...

Страница 50: ... causes and the remedial action to be taken If a fault indication continues or recurs remove the samples from the refrigerated chamber and store them in a suitable refrigerated container and contact your Thermo Service Representative TABLE 1 INSTRUMENT FUNCTION SYMPTOM CAUSE REMEDY The Cryotome does not respond when the mains power is switched on 1 The instrument is still carrying out initial test...

Страница 51: ... and clamped correctly 3 Re mount the specimen on the Cryocassette using adequate mountant 4 Increase knife angle Sections roll up 1 Incorrect gap between Anti Roll Plate and knife 2 Anti Roll Plate too low 3 Incorrect Anti Roll Plate angle 1 Adjust Anti Roll Plate gap 2 Raise the height of the Anti Roll Plate 3 Adjust the angle between the Anti Roll Plate and the knife Sections thaw when cut 1 Cu...

Страница 52: ...ing is secure Sections thick and thin 1 Blunt knife 2 Knife angle too shallow 3 Specimen not secure 4 Knife Holder is loose 1 Sharpen knife 2 Widen the knife angle 3 Re freeze and secure the specimen 4 Make sure the Knife Holder is secure on the base Sections stick to the Anti Roll Plate 1 Dirt or contaminant on the Anti Roll Plate 2 Cutting equipment not cold enough 1 Use the brush to clean the A...

Страница 53: ...elections correctly Err 2 1 Invalid pushbutton entry during Defrost Fumigate 2 Defrost is inhibited because the Cryobar and Chamber are too warm 1 Use the correct pushbutton or wait for the function to end 2 Adjust working temperature to a lower chamber temperature Err 3 Not Allocated None Err 4 1 Window lock error cannot lock or unlock 1 Make sure that the window is closed correctly 2 Contact the...

Страница 54: ...r Demand Characteristics NOMINAL a c INPUT MINIMUM VOLTAGE I max MAXIMUM VOLTAGE AT NO LOAD I max MAXIMUM CURRENT WHEN COMPRESSOR STARTS MAXIMUM SUPPLY IMPEDANCE V Hz V V A V A 230 50 60 198 264 20 1 0 115 50 60 99 121 44 0 5 Notes 1 I max Peak Current 2 Peak Current is for 22 secs max with stalled rotor 3 Voltage drop affects compressor start capability 4 Supply impedance affects compressor start...

Страница 55: ... 60 Hz German 0620 G 220 240 V 50 60 Hz French 0620 F 110 120 V 50 60 Hz 0620 110 Cryotome E 220 240 V 50 60 Hz English 0620E 220 240 V 50 60 Hz German 0620E G 220 240 V 50 60 Hz French 0620E F 110 120 V 50 60 Hz 0620E 110 7 1 7 Centre of Gravity Position see also page 5 221 mm 508 mm 317 mm WINDOW BOOST 7 1 8 Microtome Rotary rocking microtome mounted outside refrigerated chamber Total specimen a...

Страница 56: ...5 0603 8mm circular biopsy 5 0620 007 25 4mm 1 square 5 0620 001 Variety Pack 5 A77210155 MOHS Organiser Tray 1 77230046 See Catalogue for details of solid knives disposable blades additional consumables and accessories 7 3 SPARES INSTRUMENT SPARES QUANTITY PART NUMBER Anti Roll Plate Glass Disposable Knife Holder A77210086 Anti Roll Plate Perspex Solid Knife Holder 77230019 Brush P12940 Debris Tr...

Страница 57: ...condition for many years to come Warranty provisions necessarily vary to comply with differences in national and regional legislation and you can find details in your delivery documents or from your dealer or representative Please note that your warranty may be invalidated if the instrument is modified in any way accessories and reagents are used that are not approved by Thermo or the instrument i...

Страница 58: ...Shandon Limited Trading as Thermo Fisher Scientific Manufacturer s Address Tudor Road Manor Park Runcorn Cheshire WA7 1TA ENGLAND Product Description Electronic Cryostat Product Designation Cryotome E 0620E 0620E G 0620E F 0620E 110 including accessories supplied as standard and the following accessories Orientation Heads 0620 006 0620 008 Cryocassettes 603 605 77210080 0620 001 0620 007 0620 026 ...

Страница 59: ...mo Shandon Limited Trading as Thermo Fisher Scientific Manufacturer s Address Tudor Road Manor Park Runcorn Cheshire WA7 1TA ENGLAND Product Description Standard Cryostat Product Designation Cryotome 0620E 0620 G 0620 F 0620 110 including accessories supplied as standard and the following accessories Orientation Heads 0620 006 0620 008 Cryocassettes 603 605 77210080 0620 001 0620 007 0620 026 0620...

Страница 60: ...sary accompanied by a completed Decontamination Certificate Should the instrument or any part of it be received in an unclean condition or Thermo Electron Corporation consider it to be a hazard the instrument or part will be returned unrepaired at the expense of the customer It is important that the certificate is forwarded by post or fax and a copy attached to the exterior of the container Contai...

Страница 61: ...the Cryobar with a rubber band Lift the top cover and secure the window in the closed position using sticky tape Turn the instrument lowering knobs counter clockwise to lower the castors to the ground 9 Remove the back panel Refit the transit fixings as follows the red transit fixings were kept safe when the instrument was installed To avoid damage to the instrument the instrument must not be tran...

Страница 62: ...op spacer with the butterfly nut 12 Unscrew and remove the nut on top of the defrost valve Carefully remove the defrost valve from the copper tube and use cable ties to secure it to the transit plate on the rear of the microtome Place the nut washer and nameplate into a small bag and secure to the defrost valve 13 Place the wooden supports under the compressor mounting plate 14 Fit and tighten the...

Страница 63: ...and where necessary accompanied by a completed Decontamination Certificate Should the instrument or any part of it be received in an unclean condition or Thermo Electron Corporation consider it to be a hazard the instrument or part will be returned unrepaired at the expense of the customer It is important that the certificate is forwarded by post or fax and a copy attached to the exterior of the c...

Страница 64: ...e the window in the closed position using sticky tape Turn the instrument lowering knobs counter clockwise to lower the castors to the ground 10 Remove the back panel Refit the transit fixings as follows the red transit fixings were kept safe when the instrument was installed To avoid damage to the instrument the instrument must not be transported without the transit fixings fitted Contact Thermo ...

Страница 65: ...be and use cable ties to secure it to the transit plate on the rear of the microtome Place the nut washer and nameplate into a small bag and secure to the defrost valve 13 Place the wooden supports under the compressor mounting plate 14 Fit and tighten the two red handles to the screws on the base of the compressor mounting plate 15 Replace the back panel Make sure the centre bolt is also fitted ...

Страница 66: ...rior of the container Containers will not be opened until the company is in possession of the required certificate This form MUST be completed by the customer and NOT a Thermo or distributor employee If an instrument or part is to be returned to THERMO please note the following 1 If the instrument or any part of it has been exposed to or been in contact with potential pathogenic or radioactive mat...

Страница 67: ... can be used with the Cryotome cryostat If you want to use a reagent not included in this list contact your Thermo agent for advice ALWAYS REFER TO THE MATERIAL SAFETY DATA SHEET MSDS FOR THE REAGENTS USED B 2 REAGENT LIST B 2 1 Decontaminants Formalin 10 Sodium Hypochlorite 10 B 3 CLEANING AGENTS Dilute detergent Alcohol Water ...

Страница 68: ...THAT TISSUE SAMPLES MAY POSE A BIOHAZARD TAKE SUITABLE PRECAUTIONS TISSUE TYPE CHAMBER TEMPERATURE C UNFIXED TISSUE Brain 12 Liver 14 Lymph Node 14 Kidney 16 Spleen 16 Muscle 20 Thyroid 20 Skin 25 Breast 25 Breast with fat 30 or below Adipose tissue 30 or below FIXED TISSUE Fixed tissue 12 to 17 These temperatures are guidelines only Make sure that the tissue is sufficiently frozen before sectioni...

Страница 69: ...fe 46 Defrosting 46 Electrical Protection 48 Flash Testing 49 Portable Appliance Testing PAT 49 Fumigation 44 General 43 Maintenance 47 To Fit a Lamp Tube 48 To Remove the Lamp Tube 47 CONTENTS 3 CONTROLS 8 Advance Control Panel 15 Cryotome E 15 Standard Cryotome 15 Connections 18 Mains On Off Circuit Breaker 18 Power Inlet 18 Window Boost 18 Description 8 Main Control Panel 8 Chamber Controls 11 ...

Страница 70: ...des 34 To Set Up the Knife Holder and Anti Roll Plate for Standard Solid Knives 31 Warnings 27 To Remove the Transit Fixings 20 Microtome Transit Fixings 22 Refrigeration Transit Fixings 21 To Switch On and Off 26 To Switch Off 27 To Switch On 26 To Unpack 19 L LOBAT Message Code 16 M Mains On Off Circuit Breaker 18 Microtome Specification 55 O On Off Switch convention 54 OPERATION 38 Housekeeping...

Страница 71: ...me Transit Fixings 22 Refrigeration Transit Fixings 21 TROUBLE SHOOTING 50 General 50 Table 1 Instrument Function 50 Table 2 Problems when sectioning 51 52 Table 3 Error Messages 53 W WARRANTY STATEMENT 57 Waste Bottle 27 47 Weight 5 19 WELCOME 4 Introduction 4 Statement of intended use 4 Safety 4 Centre of Gravity Position 5 Electrical Earthing Requirements 5 Refrigeration information 6 Warnings ...

Страница 72: ...77210163GB Issue 3 72 ...

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