Roche MagNA Pure Compact Instrument, Руководство оператора

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How to Operate the MagNA Pure Compact Instrument 43
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2. Performing a Purification Run
Do not use the equipment in a manner not specified by the manufacturer (see pages 11-
12). Otherwise, the protection provided by the equipment might be impaired.
For kit specific details regarding the handling of Reagent Cartridges, please see the
corresponding pack insert of the kit.
Warnings and Precautions when handling the Reagent Cartridges:
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Wear protective disposable gloves, laboratory coats and eye protection when handling
samples and kit reagents, and follow usual safety precautions during handling. Avoid
contact of the reagents from the kit with skin, eyes, or mucous membranes. If contact
does occur, immediately wash with large amounts of water. Burns can occur if left
untreated. If reagents are spilled, dilute with water before wiping dry.
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Handle each Reagent Cartridge prior to use as follows:
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Always adapt the Reagent Cartridge to room temperature (30 min). If you use the
reagents at temperatures outside the recommended range, the kit may not work
well.
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Check the cartridge sealing foil for any possible damage and the cartridge for
correct filling. Do not use a cartridge that does not appear as specified.
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Always wear gloves when handling the MagNA Pure Compact reagent cartridge.
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Hold the cartridge at the barcode imprinted area and the opposite side only.
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Avoid touching the sealing foil covering the cartridge wells.
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Avoid touching the two single open wells and do not use them as handles.
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Mix the content of the cartridge wells by turning the whole cartridge upside down
several times.
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Avoid any foam formation.
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Let the fluid within the cartridge wells settle again completely. If fluid or magnetic
glass particles remain under the sealing foil, knock the cartridge bottom gently on a
flat lab bench surface. Note: Small drops may remain at the sealing foil (up to 30 µl).
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Wash hands thoroughly after handling samples and test reagents.
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Do not eat, drink, or smoke in the laboratory work area and do not pipet by mouth.
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Some buffers contain the hazardous compounds guanidine thiocyanate and guanidine
hydrochloride. Do not allow reagents containing guanidine thiocyanate to contact
sodium hypochlorite (bleach) solution or acids. These mixtures produce a highly toxic
gas.
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All mammalian (especially human) material and all resulting waste is potentially
infectious. Thoroughly clean and disinfect all work surfaces with disinfectants recom-
mended by the local authorities.
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Dispose of unused reagents and waste in accordance with country, federal, state, and
local regulations.
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Material Safety Data Sheets (MSDS) are available upon request from the local Roche
office.
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Use sterile, disposable, nuclease-free pipette tips to avoid microbial and nuclease con-
tamination.
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Do not use sharp or pointed objects near the reagent cartridge in order to prevent
damage of the sealing foil and loss of reagent.
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Do not use a kit after its expiration date.
Performing a Purification Run
MagNA Pure Compact Manual.book Seite 43 Dienstag, 28. Februar 2006 4:16 16