Roche MagNA Pure Compact Instrument, Руководство оператора

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Maintenance and Trouble Shooting 95
C
C
Poor (RT-) PCR
performance /
poor sensitivity Too much nucleic acid in PCR Check nucleic acid concentration in the eluted sam-
ples and adjust amount of eluate per PCR. Optimum:
e.g. for DNA 10-100 ng/PCR.
Use less starting material. Refer to the respective pack
insert of the reagent kit.
Not enough nucleic acid in PCR Check nucleic acid concentration in the eluted sam-
ples and adjust amount of eluate per PCR. Optimum:
e.g. for DNA 10–100 ng/PCR.
Use more starting material, refer to the respective pack
insert of the reagent kit.
Poor purity of nucleic acid Too much sample material, refer to the respective pack
insert of the reagent kit.
Storage of samples was not optimal Use fresh or frozen samples; avoid using samples that
were stored at room temperature for extended periods
of time.
Improper storage of eluate Do not store eluates at room temperature;
do not perform overnight runs.
Incorrect anticoagulant was used Use EDTA or citrated blood or plasma. Do not use
heparinized blood or plasma.
(RT-) PCR reagents and protocols
are not optimal Check (RT-) PCR reagents and protocols with a posi-
tive DNA or RNA control (
e.g. , Human Genomic DNA,
Cat. No. 1 691 112) or use an internal control.
Problem Possible cause Recommendation
Error Codes and Trouble Shooting Guide
Trouble Shooting Guide
MagNA Pure Compact Manual.book Seite 95 Dienstag, 28. Februar 2006 4:16 16