Qiagen QIAgility, Руководство пользователя

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QIAgility Software
5-64 QIAgility User Manual 11/2011
2. Enter the list of concentrations, either using the “Custom
Concentration” dialog box, or by entering the values
directly into the “Standard Data” list.
3. Dilution ratios will be automatically calculated.
4. Deselect “Use pre-mixed standard series?”.
See Section 5.6.4 for more details about setting the
standards extra volume.
5.9.3 Reagents
The default location for reagents is on the right side of the
Reagent Block. Some plates have several segments and
therefore have more than one option for the location of
reagents.
Reagents can be added directly into reaction tubes, or can be
used to make up a master mix, which can then be added to
reaction tubes. To improve precision and accuracy, it is
recommended that reagents are added to a master mix,
rather than pipetted individually. When the per-reaction
volume of reagent is <1 µl (i.e., outside the QIAgility
pipetting range), the reagent must be added to a master mix.
Reagents are set up using the “Reagent” right-hand pane. To
view the “Reagent” right-hand pane, left-click on any plate or
well that has “Reagent” function.
“Reagent” right-hand pane