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General Description
QIAgility User Manual 11/2011 3-1
3 General Description
The QIAgility System performs fully automated PCR setup.
The QIAgility System comprises the QIAgility, a computer,
and QIAgility Software. These components have been
optimized to work together across a wide variety of
applications and provide unmatched accuracy, precision,
and speed for PCR setup. There are 2 possible configurations
— with HEPA filter/UV lamp, and without HEPA filter/UV
lamp.
3.1 QIAgility principle
The QIAgility uses a single-channel pipet to automate PCR
setup — up to 96 PCR reactions can be set up in
approximately 30 minutes. Level sensing, facilitated by
conductive tips, enables high-precision pipetting.
1. The QIAgility worktable is set up with a Master Mix
Block, a Reagent Block, tips and plates/tubes. The
instrument is then calibrated.
Note : Tubes are placed into plates and are therefore
treated as plates in the software.
2. Run files are created and modified using the easy-to-use
QIAgility software. The user can download Q Protocols
from www.qiagen/goto/QIAgility and modify them
according to their needs. Wizards are also available to
guide the user step by step through developing new run
files.
3. The progress of a run can be followed in real-time. The
software highlights the position of the pipetting head on
the screen as it moves.
4. The QIAgility supports virtually all plate formats,
including Rotor-Discs for the Rotor-Gene ® Q, therefore
PCR reactions can be analyzed immediately using any
PCR cycler.