Qiagen QIAgility, Руководство пользователя

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Appendix F
QIAgility User Manual 11/2011 F-3
hand pane). When activated, the “Mix on ejection?” option is
checked by default; when deactivated, this option is
unchecked by default.
By default, this function is activated.
Allow preserved well indices
When activated a tick is visible. This function affects the
options listed in the Target Wells section of the “Reaction
Configuration” dialog box shown when editing PCR setup
liquid handling operations (displayed using the “Add” button
of the “Reaction” right-hand pane). When activated, the
“Preserve source well indices” option is presented; when
deactivated, this option is not presented.
The “Preserve source well indices” function changes the
reaction well allocation strategy used by the software for the
corresponding liquid handling operation. Normally, this
function is disabled and the software allocates reaction wells
sequentially from the first available well in the target plate
not used by previous reaction setup operations in the run.
When activated, the software attempts to use the well index
in the reaction plate corresponding to the well index of the
sample in the source plate for the reaction made using that
sample.
The software applies special rules to allow reactions which
“Preserve source well indices” and those which do not to
coexist in the same run. In short, for each reaction plate the
software determines the highest index reaction well required
by a “Preserve source well indices” liquid handling operation
and treats the next well as the first available for all other
reaction construction operations which do not “Preserve
source well indices” in that plate.
By default, this function is activated.
Require extra volume in intermediate wells
When activated a tick is visible. By defining sample banks in
reaction plates, it is possible to construct new reactions from
others previously constructed. The wells in such sample