Qiagen QIAgility, Руководство пользователя

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Instrument Setup and Calibration
7-6 QIAgility User Manual 11/2011
Check Pos. Moves the pipetting head to the defined X-
and Y-position to enable the user to
visually inspect whether this corresponds to
the center of the calibration well.
Get New Tip Collects a new (200 µl) tip.
Eject Tip
Discards the tip that is currently on the
pipetting head.
3. Select a plate from the “Plate Selection” list.
Note : Tip Rack Holders are listed as 96-well plates.
4. A schematic diagram of the selected plate is displayed
on the right. Position calibration is performed using a
‘calibration well’. This well is highlighted red.
5. Use the “Lower Tip” and “Raise Tip” buttons in the “Tip
Control” panel to move the pipetting head so that it is
just above the plate surface. Ensure that the tip is not in
contact with the plate.
Note : If there is no tip attached, the pipetting head can
only move to a limited Z-height (680). To obtain lower Z-
height positions the instrument must pick up a tip. To do
this, click on the “Get New Tip” button.
6. Adjust the “X-position” and “Y-position” in the “Plate
Origin” panel so that the pipetting head is directly above
the center of the calibration well. View the pipetting head
from the front and from the side to ensure correct
alignment.
7. Click on the “Save” button. The current position is now
saved as the calibrated value. If desired, click on the
“Check Pos” button to confirm that the correct position
has been saved.
8. To perform position calibrations on other plates, repeat
steps 3–7.
9. When position calibrations are complete, click on the
“Close” button.