Qiagen QIAgility, Руководство пользователя

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Troubleshooting
9-2 QIAgility User Manual 11/2011
9.2 Operation
Comments and suggestions
Running out of master mix
a) Master mix remains
in the tube after
completion of the
run, but insufficient
volume of master
mix was delivered to
the last reactions
aliquoted
Ensure that the height calibration of the master
mix tube is set correctly. Use manual height
calibration to get a setting closer to the tube
base without touching.
b) Correct initial
volume was
supplied or made
but all master mix is
consumed before all
reactions are made
The pipettor may be aspirating larger volumes
than expected. Ensure that the volume
calibration data are correct and have been
configured properly.
Tip reuse causes greater liquid losses on the
insides of tips due to capillary and surface
tension effects. Disable tip reuse.
Master mix may be viscous and build up on the
outside of tips, causing larger losses. Increase
the master mix extra volume configuration. See
Section 5.6.3.
c) Source reagent
remains in the tube
after completion of
the run, but the
master mix has run
out before all
reactions aliquoted
Ensure that the height calibration of all reagent
wells used for construction of the master mix is
set correctly. Use manual height calibration to
get a setting closer to the tube base without
touching.
Uneven levels in reaction tubes
a) Air bubbles trapped
in sample tubes or
master mix tubes
The liquid-level sensor functions equally well on
air bubbles or the liquid surface. Centrifuge the
sample plate to remove air bubbles.